Details
| Original language | English |
|---|---|
| Title of host publication | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI |
| Editors | Attila Tarnok, Jessica P. Houston |
| Publisher | SPIE |
| ISBN (electronic) | 9781510658714 |
| Publication status | Published - 15 Mar 2023 |
| Event | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI 2023 - San Francisco, United States Duration: 30 Jan 2023 → 1 Feb 2023 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 12383 |
| ISSN (Print) | 1605-7422 |
Abstract
The SARS-CoV-2 virus is still a challenge because of its diversity and mutations. The binding interactions of the angiotensin converting enzyme 2 (ACE2) receptor and the spike protein are relevant for the SARS-CoV-2 virus to enter the cell. Consequently, it is important and helpful to analyze binding activities and the changes in the structure of the ACE2 receptor and the spike protein. Surface enhanced Raman spectroscopy is able to analyze small concentrations of the proteins without contact, non-invasively and label-free. In this work, we present a SERS based approach in the visible wavelength range to analyze and study the binding interactions of the ACE2 receptor and the spike protein. SERS measurements of the ACE2 receptor, the spike protein and the ACE2-spike complex were performed. Additionally, an inhibitor was used to prevent the spike protein from binding to ACE2 and to compare the results. The analysis of the measured SERS spectra reveals structural differences and changes due to binding activities. Thus, we show that the performed SERS based approach can help for rapid and non-invasive analysis of binding interactions of the ACE2-spike complex and also of protein binding in general.
Keywords
- ACE2, Protein analysis, SARS-CoV2 spike protein, Surface enhanced Raman spectroscopy
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Materials Science(all)
- Biomaterials
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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- Apa
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- BibTeX
- RIS
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI. ed. / Attila Tarnok; Jessica P. Houston. SPIE, 2023. 123830B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12383).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Surface enhanced Raman spectroscopy based analysis of SARS-CoV-2 spike protein binding to ACE2 receptor
AU - Wetzel, Christoph
AU - Jansen-Olliges, Linda
AU - Zeilinger, Carsten
AU - Surup, Frank
AU - Stadler, Marc
AU - Roth, Bernhard
N1 - Funding Information: The authors thank the German Federal Ministry of Education and Research (BMBF) for supporting the research (grant ref. 13N15259). We also acknowledge the support by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453) and by FOR 5170: CytoLabs - Systematische Untersuchung und Ausbeutung von Cytochalasanen.
PY - 2023/3/15
Y1 - 2023/3/15
N2 - The SARS-CoV-2 virus is still a challenge because of its diversity and mutations. The binding interactions of the angiotensin converting enzyme 2 (ACE2) receptor and the spike protein are relevant for the SARS-CoV-2 virus to enter the cell. Consequently, it is important and helpful to analyze binding activities and the changes in the structure of the ACE2 receptor and the spike protein. Surface enhanced Raman spectroscopy is able to analyze small concentrations of the proteins without contact, non-invasively and label-free. In this work, we present a SERS based approach in the visible wavelength range to analyze and study the binding interactions of the ACE2 receptor and the spike protein. SERS measurements of the ACE2 receptor, the spike protein and the ACE2-spike complex were performed. Additionally, an inhibitor was used to prevent the spike protein from binding to ACE2 and to compare the results. The analysis of the measured SERS spectra reveals structural differences and changes due to binding activities. Thus, we show that the performed SERS based approach can help for rapid and non-invasive analysis of binding interactions of the ACE2-spike complex and also of protein binding in general.
AB - The SARS-CoV-2 virus is still a challenge because of its diversity and mutations. The binding interactions of the angiotensin converting enzyme 2 (ACE2) receptor and the spike protein are relevant for the SARS-CoV-2 virus to enter the cell. Consequently, it is important and helpful to analyze binding activities and the changes in the structure of the ACE2 receptor and the spike protein. Surface enhanced Raman spectroscopy is able to analyze small concentrations of the proteins without contact, non-invasively and label-free. In this work, we present a SERS based approach in the visible wavelength range to analyze and study the binding interactions of the ACE2 receptor and the spike protein. SERS measurements of the ACE2 receptor, the spike protein and the ACE2-spike complex were performed. Additionally, an inhibitor was used to prevent the spike protein from binding to ACE2 and to compare the results. The analysis of the measured SERS spectra reveals structural differences and changes due to binding activities. Thus, we show that the performed SERS based approach can help for rapid and non-invasive analysis of binding interactions of the ACE2-spike complex and also of protein binding in general.
KW - ACE2
KW - Protein analysis
KW - SARS-CoV2 spike protein
KW - Surface enhanced Raman spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85159653105&partnerID=8YFLogxK
U2 - 10.1117/12.2648954
DO - 10.1117/12.2648954
M3 - Conference contribution
AN - SCOPUS:85159653105
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI
A2 - Tarnok, Attila
A2 - Houston, Jessica P.
PB - SPIE
T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI 2023
Y2 - 30 January 2023 through 1 February 2023
ER -