Details
| Original language | English |
|---|---|
| Title of host publication | Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2025 |
| Editors | Jessica C. Ramella-Roman, Hui Ma, Tatiana Novikova, Daniel S. Elson, I. Alex Vitkin |
| Publisher | SPIE |
| Number of pages | 13 |
| ISBN (electronic) | 9781510683921 |
| Publication status | Published - 19 Mar 2025 |
| Event | SPIE Photonics West BiOS 2025 - San Francisco, United States Duration: 25 Jan 2025 → 31 Jan 2025 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 13322 |
| ISSN (Print) | 1605-7422 |
Abstract
We introduce a pioneering study where an optical method, Mueller matrix polarimetry, was used for the detection and distinction of bacteria biofilms, which are the main cause of implant-associated infections. However, bacteria identification so far can only be done using time-consuming molecular biological methods. The physiological form and growth of the bacterial microstructures form the basis of their polarimetric response signal. We investigated several bacterial species (Streptococcus oralis, Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis, and Porphyromonas gingivalis) grown on titanium discs, a common implant material, in a reflection mode polarimetry setup. Therefore, unlike previous studies where bacterial colonies were grown on agar plates, we grew bacteria in their clinical morphology and analysed them directly on the surface of interest. The usage of titanium medium for bacterial growth gives insights on implant longevity and performance, surface texture modifications and infection prevention strategies. From Mueller matrix element analysis, we could qualitatively distinguish the different biofilms. For a more comprehensive characterization, we also analysed bacterial monolayers to understand the polarization signal dependence on the bacterial species, and reduce confounding factors. The statistical method applied is presented, as well as typical example results through box plots and frequency distribution histograms. From Lu-Chipman decomposition parameters such as retardation, polarizance, diattenuation, and depolarization, additional distinctions were calculated for each of the species. We present decomposition imaging for each species investigated. With the results obtained, our approach has a very high potential for fast and non-destructive investigation for implant infection detection.
Keywords
- biofilms, Mueller matrix, oral Bacteria, Polarimetry, state of polarization
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Materials Science(all)
- Biomaterials
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2025. ed. / Jessica C. Ramella-Roman; Hui Ma; Tatiana Novikova; Daniel S. Elson; I. Alex Vitkin. SPIE, 2025. 1332205 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 13322).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Enhanced oral bacterial discrimination by using Mueller matrix polarimetry
AU - Sharma, Gaurav
AU - Doll-Nikutta, Katharina
AU - Thoms, Hanna
AU - Torres, Maria Leilani
AU - Roth, Bernhard
N1 - Publisher Copyright: © 2025 SPIE
PY - 2025/3/19
Y1 - 2025/3/19
N2 - We introduce a pioneering study where an optical method, Mueller matrix polarimetry, was used for the detection and distinction of bacteria biofilms, which are the main cause of implant-associated infections. However, bacteria identification so far can only be done using time-consuming molecular biological methods. The physiological form and growth of the bacterial microstructures form the basis of their polarimetric response signal. We investigated several bacterial species (Streptococcus oralis, Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis, and Porphyromonas gingivalis) grown on titanium discs, a common implant material, in a reflection mode polarimetry setup. Therefore, unlike previous studies where bacterial colonies were grown on agar plates, we grew bacteria in their clinical morphology and analysed them directly on the surface of interest. The usage of titanium medium for bacterial growth gives insights on implant longevity and performance, surface texture modifications and infection prevention strategies. From Mueller matrix element analysis, we could qualitatively distinguish the different biofilms. For a more comprehensive characterization, we also analysed bacterial monolayers to understand the polarization signal dependence on the bacterial species, and reduce confounding factors. The statistical method applied is presented, as well as typical example results through box plots and frequency distribution histograms. From Lu-Chipman decomposition parameters such as retardation, polarizance, diattenuation, and depolarization, additional distinctions were calculated for each of the species. We present decomposition imaging for each species investigated. With the results obtained, our approach has a very high potential for fast and non-destructive investigation for implant infection detection.
AB - We introduce a pioneering study where an optical method, Mueller matrix polarimetry, was used for the detection and distinction of bacteria biofilms, which are the main cause of implant-associated infections. However, bacteria identification so far can only be done using time-consuming molecular biological methods. The physiological form and growth of the bacterial microstructures form the basis of their polarimetric response signal. We investigated several bacterial species (Streptococcus oralis, Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis, and Porphyromonas gingivalis) grown on titanium discs, a common implant material, in a reflection mode polarimetry setup. Therefore, unlike previous studies where bacterial colonies were grown on agar plates, we grew bacteria in their clinical morphology and analysed them directly on the surface of interest. The usage of titanium medium for bacterial growth gives insights on implant longevity and performance, surface texture modifications and infection prevention strategies. From Mueller matrix element analysis, we could qualitatively distinguish the different biofilms. For a more comprehensive characterization, we also analysed bacterial monolayers to understand the polarization signal dependence on the bacterial species, and reduce confounding factors. The statistical method applied is presented, as well as typical example results through box plots and frequency distribution histograms. From Lu-Chipman decomposition parameters such as retardation, polarizance, diattenuation, and depolarization, additional distinctions were calculated for each of the species. We present decomposition imaging for each species investigated. With the results obtained, our approach has a very high potential for fast and non-destructive investigation for implant infection detection.
KW - biofilms
KW - Mueller matrix
KW - oral Bacteria
KW - Polarimetry
KW - state of polarization
UR - http://www.scopus.com/inward/record.url?scp=105002342253&partnerID=8YFLogxK
U2 - 10.1117/12.3041861
DO - 10.1117/12.3041861
M3 - Conference contribution
AN - SCOPUS:105002342253
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2025
A2 - Ramella-Roman, Jessica C.
A2 - Ma, Hui
A2 - Novikova, Tatiana
A2 - Elson, Daniel S.
A2 - Vitkin, I. Alex
PB - SPIE
T2 - SPIE Photonics West BiOS 2025
Y2 - 25 January 2025 through 31 January 2025
ER -