Details
| Original language | English |
|---|---|
| Title of host publication | Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2025 |
| Editors | Jessica C. Ramella-Roman, Hui Ma, Tatiana Novikova, Daniel S. Elson, I. Alex Vitkin |
| Publisher | SPIE |
| ISBN (electronic) | 9781510683921 |
| Publication status | Published - 25 Jan 2025 |
| Event | Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2025 - San Francisco, United States Duration: 25 Jan 2025 → 31 Jan 2025 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 13322 |
| ISSN (Print) | 1605-7422 |
Abstract
Mueller Matrix Polarimetry (MMP) has evolved into a prominent area of research, with focus on identifying microstructural changes in bio tissues. This is done by investigating light properties, which is especially useful in early detection of brain cell degradation, among others. We investigated the application of MMP to mouse brain tissue containing amyloid-beta plaques. Alzheimer's disease is widely known for progressing with the production and appearance of the beta plaques. The investigated sample glass slides contained paraffin embedded brain tissue. The tissues were taken at various stages of ageing, i.e. 75, 100, 125, 150, 175, 200, and 225 days. Paraffin tissue blocks were used as an additional sample set for comparison. We set up the experimental Mueller matrix polarimetry to consist of three illuminating laser wavelengths (445 nm, 532 nm, 632 nm). We performed a comparative analysis based on the Mueller matrix elements for each age category and highlighted the importance of certain elements, e.g. m44, for further analysis. We also compared the trends of decomposition parameters and could correlate them with the ageing. Contrary to previous studies, we also report on retardation, diattenuation and polarizance changes. From the higher order statistics, we concluded that mean and standard deviation remained constant across the ages. Skewness values were positive and increased as the age progressed, whereas kurtosis decreased with age. The large available dataset opens the possibility of implementing machine learning methods to assist clinical diagnosis.
Keywords
- Alzeihmers, brain tissue, light polarization, polarimtery
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Materials Science(all)
- Biomaterials
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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- Apa
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- BibTeX
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Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2025. ed. / Jessica C. Ramella-Roman; Hui Ma; Tatiana Novikova; Daniel S. Elson; I. Alex Vitkin. SPIE, 2025. 133220C (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 13322).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Mueller matrix polarimetry for mouse brain tissue analysis
AU - Sharma, Gaurav
AU - Siby, Melbin
AU - Pahnke, Jens
AU - Roth, Bernhard
N1 - Publisher Copyright: © 2025 SPIE
PY - 2025/1/25
Y1 - 2025/1/25
N2 - Mueller Matrix Polarimetry (MMP) has evolved into a prominent area of research, with focus on identifying microstructural changes in bio tissues. This is done by investigating light properties, which is especially useful in early detection of brain cell degradation, among others. We investigated the application of MMP to mouse brain tissue containing amyloid-beta plaques. Alzheimer's disease is widely known for progressing with the production and appearance of the beta plaques. The investigated sample glass slides contained paraffin embedded brain tissue. The tissues were taken at various stages of ageing, i.e. 75, 100, 125, 150, 175, 200, and 225 days. Paraffin tissue blocks were used as an additional sample set for comparison. We set up the experimental Mueller matrix polarimetry to consist of three illuminating laser wavelengths (445 nm, 532 nm, 632 nm). We performed a comparative analysis based on the Mueller matrix elements for each age category and highlighted the importance of certain elements, e.g. m44, for further analysis. We also compared the trends of decomposition parameters and could correlate them with the ageing. Contrary to previous studies, we also report on retardation, diattenuation and polarizance changes. From the higher order statistics, we concluded that mean and standard deviation remained constant across the ages. Skewness values were positive and increased as the age progressed, whereas kurtosis decreased with age. The large available dataset opens the possibility of implementing machine learning methods to assist clinical diagnosis.
AB - Mueller Matrix Polarimetry (MMP) has evolved into a prominent area of research, with focus on identifying microstructural changes in bio tissues. This is done by investigating light properties, which is especially useful in early detection of brain cell degradation, among others. We investigated the application of MMP to mouse brain tissue containing amyloid-beta plaques. Alzheimer's disease is widely known for progressing with the production and appearance of the beta plaques. The investigated sample glass slides contained paraffin embedded brain tissue. The tissues were taken at various stages of ageing, i.e. 75, 100, 125, 150, 175, 200, and 225 days. Paraffin tissue blocks were used as an additional sample set for comparison. We set up the experimental Mueller matrix polarimetry to consist of three illuminating laser wavelengths (445 nm, 532 nm, 632 nm). We performed a comparative analysis based on the Mueller matrix elements for each age category and highlighted the importance of certain elements, e.g. m44, for further analysis. We also compared the trends of decomposition parameters and could correlate them with the ageing. Contrary to previous studies, we also report on retardation, diattenuation and polarizance changes. From the higher order statistics, we concluded that mean and standard deviation remained constant across the ages. Skewness values were positive and increased as the age progressed, whereas kurtosis decreased with age. The large available dataset opens the possibility of implementing machine learning methods to assist clinical diagnosis.
KW - Alzeihmers
KW - brain tissue
KW - light polarization
KW - polarimtery
UR - http://www.scopus.com/inward/record.url?scp=105002297088&partnerID=8YFLogxK
U2 - 10.1117/12.3042290
DO - 10.1117/12.3042290
M3 - Conference contribution
AN - SCOPUS:105002297088
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2025
A2 - Ramella-Roman, Jessica C.
A2 - Ma, Hui
A2 - Novikova, Tatiana
A2 - Elson, Daniel S.
A2 - Vitkin, I. Alex
PB - SPIE
T2 - Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2025
Y2 - 25 January 2025 through 31 January 2025
ER -