Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant

Research output: Chapter in book/report/conference proceedingContribution to book/anthologyResearchpeer review

Authors

Research Organisations

External Research Organisations

  • Karlsruhe Institute of Technology (KIT)
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Details

Original languageEnglish
Title of host publicationStem Cell Mobilization
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages97-107
Number of pages11
Edition1
ISBN (electronic)978-1-4939-9574-5
ISBN (print)978-1-4939-9573-8, 978-1-4939-9576-9
Publication statusPublished - 14 Jun 2019

Publication series

NameMethods in Molecular Biology
Volume2017
ISSN (Print)1064-3745
ISSN (electronic)1940-6029

Abstract

In leukemia, leukemic cells hijack the hematopoietic stem cell (HSC) microenvironment in the bone marrow—the so-called stem cell niche—by flooding the niche with clonal progeny of leukemic cells. They can exploit signaling pathways which are critical for HSC development to support their own survival, homing, and maintenance. These interactions of leukemic cells with the microenvironment have an impact on therapy progress and patient outcome. Therefore, signals for homing and anchorage of leukemic cells to the bone marrow have to be investigated by using tools that allow the migration of cells toward critical signals. Here, we describe an in vitro migration assay for leukemic cells toward a chemoattractant in a 3D environment exemplified by migration of the cell line OCI-AML3 to a CXC motif chemokine ligand 12 (CXCL12) gradient. For this purpose, a chemotaxis slide is filled with a hydrogel system mimicking the extracellular matrix in vivo. The cells are encapsulated into the hydrogel network during polymerization, and a CXCL12 gradient is introduced in the enclosed chambers to trigger migration. Cell migration in the 3D network of the hydrogel is monitored by time-lapse microscopy. We describe the experimental setup and the tools for cell tracking and data analysis.

Keywords

    Leukemic cells, Migration, 3D matrix, mu-Slides, CXCR4/CXCL12 axis, Chemokine gradient, μ-Slides

ASJC Scopus subject areas

Cite this

Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant. / Zippel, Sabrina; Raic, Annamarija; Lee-Thedieck, Cornelia.
Stem Cell Mobilization: Methods and Protocols. 1. ed. Humana Press, 2019. p. 97-107 (Methods in Molecular Biology; Vol. 2017).

Research output: Chapter in book/report/conference proceedingContribution to book/anthologyResearchpeer review

Zippel, S, Raic, A & Lee-Thedieck, C 2019, Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant. in Stem Cell Mobilization: Methods and Protocols. 1 edn, Methods in Molecular Biology, vol. 2017, Humana Press, pp. 97-107. https://doi.org/10.1007/978-1-4939-9574-5_8
Zippel, S., Raic, A., & Lee-Thedieck, C. (2019). Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant. In Stem Cell Mobilization: Methods and Protocols (1 ed., pp. 97-107). (Methods in Molecular Biology; Vol. 2017). Humana Press. https://doi.org/10.1007/978-1-4939-9574-5_8
Zippel S, Raic A, Lee-Thedieck C. Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant. In Stem Cell Mobilization: Methods and Protocols. 1 ed. Humana Press. 2019. p. 97-107. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-9574-5_8
Zippel, Sabrina ; Raic, Annamarija ; Lee-Thedieck, Cornelia. / Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant. Stem Cell Mobilization: Methods and Protocols. 1. ed. Humana Press, 2019. pp. 97-107 (Methods in Molecular Biology).
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