Details
| Original language | English |
|---|---|
| Article number | 025033 |
| Journal | BIOFABRICATION |
| Volume | 17 |
| Issue number | 2 |
| Publication status | Published - 27 Mar 2025 |
Abstract
Patients suffering from large bone defects are in urgent need of suitable bone replacements. Besides biocompatibility, such replacements need to mimic the 3D architecture of bone and match chemical, mechanical and biological properties, ideally promoting ossification. As natural bone mainly contains collagen type I and carbonate hydroxyapatite, a 3D-printable biomaterial consisting of methacrylated gelatin (GelMA) and nanohydroxyapatite (nHAp) would be beneficial to mimic the composition and shape of natural bone. So far, such nanocomposite hydrogels (NCH) suffered from unsatisfactory rheological properties making them unsuitable for extrusion-based 3D printing with high structural fidelity. In this study, we introduce a novel GelMA/nHAp NCH composition, incorporating the rheological modifier carbomer to improve rheological properties and addressing the challenge of calcium cations released from nHAp that hinder GelMA gelation. Leveraging its shear-thinning and self-healing properties, the NCH ink retains its shape and forms cohesive structures after deposition, which can be permanently stabilized by subsequent UV crosslinking. Consequently, the NCH enables the printing of 3D structures with high shape fidelity in all dimensions, including the z-direction, allowing the fabrication of highly macroporous constructs. Both the uncured and the UV crosslinked NCH behave like a viscoelastic solid, with G′> G″ at deformations up to 100-200 %. After UV crosslinking, the NCH can, depending on the GelMA concentration, reach storage moduli of approximately 10 to over 100 kPa and a mean Young’s Modulus of about 70 kPa. The printed scaffolds permit not only cell survival but also osteogenic differentiation, highlighting their potential for bone tissue engineering.
Keywords
- 3D printing, biomaterials, bone mimetics, nanocomposite hydrogels, osteogenic differentiation
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
Research Area (based on ÖFOS 2012)
- NATURAL SCIENCES
- Biology
- Biology
- Cell biology
- HUMAN MEDICINE, HEALTH SCIENCES
- Medical Biotechnology
- Medical Biotechnology
- Tissue engineering
- NATURAL SCIENCES
- Chemistry
- Chemistry
- Macromolecular chemistry
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In: BIOFABRICATION, Vol. 17, No. 2, 025033, 27.03.2025.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Enhanced gelatin methacryloyl nanohydroxyapatite hydrogel for high-fidelity 3D printing of bone tissue engineering scaffolds
AU - Naolou, Toufik
AU - Schadzek, Nadine
AU - Hornbostel, Jan Mathis
AU - Pepelanova, Iliyana
AU - Frommer, Miriam
AU - Lötz, Franziska
AU - Sauheitl, Leopold
AU - Dultz, Stefan
AU - Felde, Vincent J.M.N.L.
AU - Myklebost, Ola
AU - Lee-Thedieck, Cornelia
N1 - Publisher Copyright: © 2025 The Author(s). Published by IOP Publishing Ltd.
PY - 2025/3/27
Y1 - 2025/3/27
N2 - Patients suffering from large bone defects are in urgent need of suitable bone replacements. Besides biocompatibility, such replacements need to mimic the 3D architecture of bone and match chemical, mechanical and biological properties, ideally promoting ossification. As natural bone mainly contains collagen type I and carbonate hydroxyapatite, a 3D-printable biomaterial consisting of methacrylated gelatin (GelMA) and nanohydroxyapatite (nHAp) would be beneficial to mimic the composition and shape of natural bone. So far, such nanocomposite hydrogels (NCH) suffered from unsatisfactory rheological properties making them unsuitable for extrusion-based 3D printing with high structural fidelity. In this study, we introduce a novel GelMA/nHAp NCH composition, incorporating the rheological modifier carbomer to improve rheological properties and addressing the challenge of calcium cations released from nHAp that hinder GelMA gelation. Leveraging its shear-thinning and self-healing properties, the NCH ink retains its shape and forms cohesive structures after deposition, which can be permanently stabilized by subsequent UV crosslinking. Consequently, the NCH enables the printing of 3D structures with high shape fidelity in all dimensions, including the z-direction, allowing the fabrication of highly macroporous constructs. Both the uncured and the UV crosslinked NCH behave like a viscoelastic solid, with G′> G″ at deformations up to 100-200 %. After UV crosslinking, the NCH can, depending on the GelMA concentration, reach storage moduli of approximately 10 to over 100 kPa and a mean Young’s Modulus of about 70 kPa. The printed scaffolds permit not only cell survival but also osteogenic differentiation, highlighting their potential for bone tissue engineering.
AB - Patients suffering from large bone defects are in urgent need of suitable bone replacements. Besides biocompatibility, such replacements need to mimic the 3D architecture of bone and match chemical, mechanical and biological properties, ideally promoting ossification. As natural bone mainly contains collagen type I and carbonate hydroxyapatite, a 3D-printable biomaterial consisting of methacrylated gelatin (GelMA) and nanohydroxyapatite (nHAp) would be beneficial to mimic the composition and shape of natural bone. So far, such nanocomposite hydrogels (NCH) suffered from unsatisfactory rheological properties making them unsuitable for extrusion-based 3D printing with high structural fidelity. In this study, we introduce a novel GelMA/nHAp NCH composition, incorporating the rheological modifier carbomer to improve rheological properties and addressing the challenge of calcium cations released from nHAp that hinder GelMA gelation. Leveraging its shear-thinning and self-healing properties, the NCH ink retains its shape and forms cohesive structures after deposition, which can be permanently stabilized by subsequent UV crosslinking. Consequently, the NCH enables the printing of 3D structures with high shape fidelity in all dimensions, including the z-direction, allowing the fabrication of highly macroporous constructs. Both the uncured and the UV crosslinked NCH behave like a viscoelastic solid, with G′> G″ at deformations up to 100-200 %. After UV crosslinking, the NCH can, depending on the GelMA concentration, reach storage moduli of approximately 10 to over 100 kPa and a mean Young’s Modulus of about 70 kPa. The printed scaffolds permit not only cell survival but also osteogenic differentiation, highlighting their potential for bone tissue engineering.
KW - 3D printing
KW - biomaterials
KW - bone mimetics
KW - nanocomposite hydrogels
KW - osteogenic differentiation
UR - http://www.scopus.com/inward/record.url?scp=105002298172&partnerID=8YFLogxK
U2 - 10.1088/1758-5090/adbb90
DO - 10.1088/1758-5090/adbb90
M3 - Article
C2 - 40020249
AN - SCOPUS:105002298172
VL - 17
JO - BIOFABRICATION
JF - BIOFABRICATION
SN - 1758-5082
IS - 2
M1 - 025033
ER -