Iron Nanoparticle Composite Hydrogels for Studying Effects of Iron Ion Release on Red Blood Cell In Vitro Production

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Katharina Brändle
  • Timna C. Bergmann
  • Annamarija Raic
  • Yaya Li
  • Nina Million
  • Christoph Rehbock
  • Stephan Barcikowski
  • Cornelia Lee-Thedieck

Research Organisations

External Research Organisations

  • University of Duisburg-Essen
  • Karlsruhe Institute of Technology (KIT)
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Details

Original languageEnglish
Pages (from-to)4766-4778
Number of pages13
JournalACS Applied Bio Materials
Volume3
Issue number8
Early online date1 Jul 2020
Publication statusPublished - 17 Aug 2020

Abstract

Growing numbers of complex surgical interventions increase the need for blood transfusions, which cannot be fulfilled by the number of donors. Therefore, the interest in producing erythrocytes from their precursors-the hematopoietic stem and progenitor cells (HSPCs)-in laboratories is rising. To enable this, in vitro systems are needed, which allow analysis of the effects of essential factors such as iron on erythroid development. For this purpose, iron ion-releasing systems based on poly(ethylene glycol) (PEG)-iron nanocomposites are developed to assess if gradual iron release improves iron bioavailability during in vitro erythroid differentiation. The nanocomposites are synthesized using surfactant-free pulsed laser ablation of iron directly in the PEG solution. The iron concentrations released from the material are sufficient to influence in vitro erythropoiesis. In this way, the production of erythroid cells cultured on flat PEG-iron nanocomposite hydrogel pads can be enhanced. In contrast, erythroid differentiation is not enhanced in the biomimetic macroporous 3D composite scaffolds, possibly because of local iron overload within the pores of the system. In conclusion, the developed iron nanoparticle-PEG composite hydrogel allows constant iron ion release and thus paves the way (i) to understand the role of iron during erythropoiesis and (ii) toward the development of biomaterials with a controlled iron release for directing erythropoiesis in culture.

Keywords

    erythropoiesis, hematopoietic stem cell, hydrogel, iron nanoparticle, iron release system, red blood cell production, regenerative medicine, stem cells

ASJC Scopus subject areas

Cite this

Iron Nanoparticle Composite Hydrogels for Studying Effects of Iron Ion Release on Red Blood Cell In Vitro Production. / Brändle, Katharina; Bergmann, Timna C.; Raic, Annamarija et al.
In: ACS Applied Bio Materials, Vol. 3, No. 8, 17.08.2020, p. 4766-4778.

Research output: Contribution to journalArticleResearchpeer review

Brändle, K, Bergmann, TC, Raic, A, Li, Y, Million, N, Rehbock, C, Barcikowski, S & Lee-Thedieck, C 2020, 'Iron Nanoparticle Composite Hydrogels for Studying Effects of Iron Ion Release on Red Blood Cell In Vitro Production', ACS Applied Bio Materials, vol. 3, no. 8, pp. 4766-4778. https://doi.org/10.5445/IR/1000125240, https://doi.org/10.1021/acsabm.0c00297
Brändle, K., Bergmann, T. C., Raic, A., Li, Y., Million, N., Rehbock, C., Barcikowski, S., & Lee-Thedieck, C. (2020). Iron Nanoparticle Composite Hydrogels for Studying Effects of Iron Ion Release on Red Blood Cell In Vitro Production. ACS Applied Bio Materials, 3(8), 4766-4778. https://doi.org/10.5445/IR/1000125240, https://doi.org/10.1021/acsabm.0c00297
Brändle K, Bergmann TC, Raic A, Li Y, Million N, Rehbock C et al. Iron Nanoparticle Composite Hydrogels for Studying Effects of Iron Ion Release on Red Blood Cell In Vitro Production. ACS Applied Bio Materials. 2020 Aug 17;3(8):4766-4778. Epub 2020 Jul 1. doi: 10.5445/IR/1000125240, 10.1021/acsabm.0c00297
Brändle, Katharina ; Bergmann, Timna C. ; Raic, Annamarija et al. / Iron Nanoparticle Composite Hydrogels for Studying Effects of Iron Ion Release on Red Blood Cell In Vitro Production. In: ACS Applied Bio Materials. 2020 ; Vol. 3, No. 8. pp. 4766-4778.
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title = "Iron Nanoparticle Composite Hydrogels for Studying Effects of Iron Ion Release on Red Blood Cell In Vitro Production",
abstract = "Growing numbers of complex surgical interventions increase the need for blood transfusions, which cannot be fulfilled by the number of donors. Therefore, the interest in producing erythrocytes from their precursors-the hematopoietic stem and progenitor cells (HSPCs)-in laboratories is rising. To enable this, in vitro systems are needed, which allow analysis of the effects of essential factors such as iron on erythroid development. For this purpose, iron ion-releasing systems based on poly(ethylene glycol) (PEG)-iron nanocomposites are developed to assess if gradual iron release improves iron bioavailability during in vitro erythroid differentiation. The nanocomposites are synthesized using surfactant-free pulsed laser ablation of iron directly in the PEG solution. The iron concentrations released from the material are sufficient to influence in vitro erythropoiesis. In this way, the production of erythroid cells cultured on flat PEG-iron nanocomposite hydrogel pads can be enhanced. In contrast, erythroid differentiation is not enhanced in the biomimetic macroporous 3D composite scaffolds, possibly because of local iron overload within the pores of the system. In conclusion, the developed iron nanoparticle-PEG composite hydrogel allows constant iron ion release and thus paves the way (i) to understand the role of iron during erythropoiesis and (ii) toward the development of biomaterials with a controlled iron release for directing erythropoiesis in culture.",
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note = "Funding information: We thank Marita Heinle (Karlsruhe Institute of Technology) for ICP-OES measurements and Chandralekha Chatterjee (Leibniz University Hannover) for proof reading the manuscript. The project was supported by the BMBF NanoMatFutur Program (FKZ 13N12968 and 13XP5076A). This project has received funding from the European Research Council (ERC) under the European Union{\textquoteright}s Horizon 2020 research and innovation programme (grant agreement no. 757490). C.L.T. acknowledges support from the framework of the SMART BIOTECS alliance between the Technische Universit{\"a}t Braunschweig and the Leibniz Universit{\"a}t Hannover. This initiative is supported by the Ministry of Science and Culture (MWK) of Lower Saxony, Germany. Further thanks goes to the “Deutsche Forschungsgemeinschaft (DFG) for funding within the priority program SPP 1327. Y.L. acknowledges the Chinese Research Council for financial support.",
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AU - Brändle, Katharina

AU - Bergmann, Timna C.

AU - Raic, Annamarija

AU - Li, Yaya

AU - Million, Nina

AU - Rehbock, Christoph

AU - Barcikowski, Stephan

AU - Lee-Thedieck, Cornelia

N1 - Funding information: We thank Marita Heinle (Karlsruhe Institute of Technology) for ICP-OES measurements and Chandralekha Chatterjee (Leibniz University Hannover) for proof reading the manuscript. The project was supported by the BMBF NanoMatFutur Program (FKZ 13N12968 and 13XP5076A). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 757490). C.L.T. acknowledges support from the framework of the SMART BIOTECS alliance between the Technische Universität Braunschweig and the Leibniz Universität Hannover. This initiative is supported by the Ministry of Science and Culture (MWK) of Lower Saxony, Germany. Further thanks goes to the “Deutsche Forschungsgemeinschaft (DFG) for funding within the priority program SPP 1327. Y.L. acknowledges the Chinese Research Council for financial support.

PY - 2020/8/17

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