TY - JOUR

T1 - Iron Nanoparticle Composite Hydrogels for Studying Effects of Iron Ion Release on Red Blood Cell In Vitro Production

AU - Brändle, Katharina

AU - Bergmann, Timna C.

AU - Raic, Annamarija

AU - Li, Yaya

AU - Million, Nina

AU - Rehbock, Christoph

AU - Barcikowski, Stephan

AU - Lee-Thedieck, Cornelia

N1 - Funding information: We thank Marita Heinle (Karlsruhe Institute of Technology) for ICP-OES measurements and Chandralekha Chatterjee (Leibniz University Hannover) for proof reading the manuscript. The project was supported by the BMBF NanoMatFutur Program (FKZ 13N12968 and 13XP5076A). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 757490). C.L.T. acknowledges support from the framework of the SMART BIOTECS alliance between the Technische Universität Braunschweig and the Leibniz Universität Hannover. This initiative is supported by the Ministry of Science and Culture (MWK) of Lower Saxony, Germany. Further thanks goes to the “Deutsche Forschungsgemeinschaft (DFG) for funding within the priority program SPP 1327. Y.L. acknowledges the Chinese Research Council for financial support.

PY - 2020/8/17

Y1 - 2020/8/17

N2 - Growing numbers of complex surgical interventions increase the need for blood transfusions, which cannot be fulfilled by the number of donors. Therefore, the interest in producing erythrocytes from their precursors-the hematopoietic stem and progenitor cells (HSPCs)-in laboratories is rising. To enable this, in vitro systems are needed, which allow analysis of the effects of essential factors such as iron on erythroid development. For this purpose, iron ion-releasing systems based on poly(ethylene glycol) (PEG)-iron nanocomposites are developed to assess if gradual iron release improves iron bioavailability during in vitro erythroid differentiation. The nanocomposites are synthesized using surfactant-free pulsed laser ablation of iron directly in the PEG solution. The iron concentrations released from the material are sufficient to influence in vitro erythropoiesis. In this way, the production of erythroid cells cultured on flat PEG-iron nanocomposite hydrogel pads can be enhanced. In contrast, erythroid differentiation is not enhanced in the biomimetic macroporous 3D composite scaffolds, possibly because of local iron overload within the pores of the system. In conclusion, the developed iron nanoparticle-PEG composite hydrogel allows constant iron ion release and thus paves the way (i) to understand the role of iron during erythropoiesis and (ii) toward the development of biomaterials with a controlled iron release for directing erythropoiesis in culture.

AB - Growing numbers of complex surgical interventions increase the need for blood transfusions, which cannot be fulfilled by the number of donors. Therefore, the interest in producing erythrocytes from their precursors-the hematopoietic stem and progenitor cells (HSPCs)-in laboratories is rising. To enable this, in vitro systems are needed, which allow analysis of the effects of essential factors such as iron on erythroid development. For this purpose, iron ion-releasing systems based on poly(ethylene glycol) (PEG)-iron nanocomposites are developed to assess if gradual iron release improves iron bioavailability during in vitro erythroid differentiation. The nanocomposites are synthesized using surfactant-free pulsed laser ablation of iron directly in the PEG solution. The iron concentrations released from the material are sufficient to influence in vitro erythropoiesis. In this way, the production of erythroid cells cultured on flat PEG-iron nanocomposite hydrogel pads can be enhanced. In contrast, erythroid differentiation is not enhanced in the biomimetic macroporous 3D composite scaffolds, possibly because of local iron overload within the pores of the system. In conclusion, the developed iron nanoparticle-PEG composite hydrogel allows constant iron ion release and thus paves the way (i) to understand the role of iron during erythropoiesis and (ii) toward the development of biomaterials with a controlled iron release for directing erythropoiesis in culture.

KW - erythropoiesis

KW - hematopoietic stem cell

KW - hydrogel

KW - iron nanoparticle

KW - iron release system

KW - red blood cell production

KW - regenerative medicine

KW - stem cells

UR - http://www.scopus.com/inward/record.url?scp=85091041545&partnerID=8YFLogxK

U2 - 10.5445/IR/1000125240

DO - 10.5445/IR/1000125240

M3 - Article

VL - 3

SP - 4766

EP - 4778

JO - ACS Applied Bio Materials

JF - ACS Applied Bio Materials

IS - 8

ER -