Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 275-281 |
| Seitenumfang | 7 |
| Fachzeitschrift | Biosensors and Bioelectronics |
| Jahrgang | 9 |
| Ausgabenummer | 4-5 |
| Publikationsstatus | Veröffentlicht - 1994 |
| Extern publiziert | Ja |
Abstract
A field-effect transistor (FET) biosensor for lactose is presented. The bioFET device employs a bi-enzyme system consisting of β-galactosidase and glucose dehydrogenase co-immobilized on the surface of the FET gate. The sensor response was measured in a differential mode, wherein the source voltage in the bioFET is compared with that in a pH-sensitive FET sensor placed next to it. The sensor produced a rapid and reproducible response to lactose. The response was affected by several factors such as stirring, NAD+ concentration, pH and buffer capacity. The optimum conditions for the performance of the bioFET were investigated.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biophysik
- Ingenieurwesen (insg.)
- Biomedizintechnik
- Chemie (insg.)
- Elektrochemie
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in: Biosensors and Bioelectronics, Jahrgang 9, Nr. 4-5, 1994, S. 275-281.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - A bio-FET sensor for lactose based on co-immobilized β-galactosidase/glucose dehydrogenase
AU - Sevilla, Fortunato
AU - Kullick, Thomas
AU - Scheper, Thomas
PY - 1994
Y1 - 1994
N2 - A field-effect transistor (FET) biosensor for lactose is presented. The bioFET device employs a bi-enzyme system consisting of β-galactosidase and glucose dehydrogenase co-immobilized on the surface of the FET gate. The sensor response was measured in a differential mode, wherein the source voltage in the bioFET is compared with that in a pH-sensitive FET sensor placed next to it. The sensor produced a rapid and reproducible response to lactose. The response was affected by several factors such as stirring, NAD+ concentration, pH and buffer capacity. The optimum conditions for the performance of the bioFET were investigated.
AB - A field-effect transistor (FET) biosensor for lactose is presented. The bioFET device employs a bi-enzyme system consisting of β-galactosidase and glucose dehydrogenase co-immobilized on the surface of the FET gate. The sensor response was measured in a differential mode, wherein the source voltage in the bioFET is compared with that in a pH-sensitive FET sensor placed next to it. The sensor produced a rapid and reproducible response to lactose. The response was affected by several factors such as stirring, NAD+ concentration, pH and buffer capacity. The optimum conditions for the performance of the bioFET were investigated.
KW - bioFET sensor
KW - co-immobilized β-galactosidase/glucose dehydrogenase
KW - enzyme FET sensor
KW - lactose sensor
UR - http://www.scopus.com/inward/record.url?scp=0027936425&partnerID=8YFLogxK
U2 - 10.1016/0956-5663(94)80024-3
DO - 10.1016/0956-5663(94)80024-3
M3 - Article
AN - SCOPUS:0027936425
VL - 9
SP - 275
EP - 281
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 4-5
ER -