Details
| Original language | English |
|---|---|
| Pages (from-to) | 275-281 |
| Number of pages | 7 |
| Journal | Biosensors and Bioelectronics |
| Volume | 9 |
| Issue number | 4-5 |
| Publication status | Published - 1994 |
| Externally published | Yes |
Abstract
A field-effect transistor (FET) biosensor for lactose is presented. The bioFET device employs a bi-enzyme system consisting of β-galactosidase and glucose dehydrogenase co-immobilized on the surface of the FET gate. The sensor response was measured in a differential mode, wherein the source voltage in the bioFET is compared with that in a pH-sensitive FET sensor placed next to it. The sensor produced a rapid and reproducible response to lactose. The response was affected by several factors such as stirring, NAD+ concentration, pH and buffer capacity. The optimum conditions for the performance of the bioFET were investigated.
Keywords
- bioFET sensor, co-immobilized β-galactosidase/glucose dehydrogenase, enzyme FET sensor, lactose sensor
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Engineering(all)
- Biomedical Engineering
- Chemistry(all)
- Electrochemistry
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In: Biosensors and Bioelectronics, Vol. 9, No. 4-5, 1994, p. 275-281.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A bio-FET sensor for lactose based on co-immobilized β-galactosidase/glucose dehydrogenase
AU - Sevilla, Fortunato
AU - Kullick, Thomas
AU - Scheper, Thomas
PY - 1994
Y1 - 1994
N2 - A field-effect transistor (FET) biosensor for lactose is presented. The bioFET device employs a bi-enzyme system consisting of β-galactosidase and glucose dehydrogenase co-immobilized on the surface of the FET gate. The sensor response was measured in a differential mode, wherein the source voltage in the bioFET is compared with that in a pH-sensitive FET sensor placed next to it. The sensor produced a rapid and reproducible response to lactose. The response was affected by several factors such as stirring, NAD+ concentration, pH and buffer capacity. The optimum conditions for the performance of the bioFET were investigated.
AB - A field-effect transistor (FET) biosensor for lactose is presented. The bioFET device employs a bi-enzyme system consisting of β-galactosidase and glucose dehydrogenase co-immobilized on the surface of the FET gate. The sensor response was measured in a differential mode, wherein the source voltage in the bioFET is compared with that in a pH-sensitive FET sensor placed next to it. The sensor produced a rapid and reproducible response to lactose. The response was affected by several factors such as stirring, NAD+ concentration, pH and buffer capacity. The optimum conditions for the performance of the bioFET were investigated.
KW - bioFET sensor
KW - co-immobilized β-galactosidase/glucose dehydrogenase
KW - enzyme FET sensor
KW - lactose sensor
UR - http://www.scopus.com/inward/record.url?scp=0027936425&partnerID=8YFLogxK
U2 - 10.1016/0956-5663(94)80024-3
DO - 10.1016/0956-5663(94)80024-3
M3 - Article
AN - SCOPUS:0027936425
VL - 9
SP - 275
EP - 281
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 4-5
ER -