Details
| Original language | English |
|---|---|
| Pages (from-to) | 223-229 |
| Number of pages | 7 |
| Journal | Analytica chimica acta |
| Volume | 255 |
| Issue number | 2 |
| Publication status | Published - 24 Dec 1991 |
Abstract
A fibre-optic biosensor based on the fluorimetric detection of NAD(P)H consumed or generated during enzymatic reactions is described. The design combines a miniaturized fluorimeter with a low-volume membrane reactor and utilizes flow-injection analysis principles. Two types of membrane reactor were developed and tested. In the first version, enzymes and a macromolecular nicotinamide adenine dinucleotide coenzyme derivative of polythyleneglycol, PEG-N6-(2-aminoethyl)-NAD(P)(H), are retained in the direct vicinity of the sensor tip. In the second version, a solid-supported liquid membrane was used to retain native coenzyme, while substrates and products were transported via selective carriers through an organic membrane barrier. The automated determination of phenylpyruvate, pyruvate, lactate, formate, ethanol and mannitol concentrations in model media was possible in the range of 50-1500 μmol l-1. The usefulness of the biosensor concept reported for real process monitoring in biotechnology or for medical purposes was demonstrated on different examples including the determination of ethanol for Saccharomyces cerevisiae cultivation.
Keywords
- Biosensors, Enzyme reactor, Ethanol, Fibre-optic sensor, Fluorimetry, Formate, Glucose, Lactate, Mannitol, Phenylpyruvate, Pyruvate
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Environmental Science(all)
- Environmental Chemistry
- Chemistry(all)
- Spectroscopy
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In: Analytica chimica acta, Vol. 255, No. 2, 24.12.1991, p. 223-229.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Two fibre-optic sensors with confined enzymes and coenzymes: development and application
AU - Schelp, C.
AU - Scheper, Th
AU - Bückmann, F.
AU - Reardon, K. F.
PY - 1991/12/24
Y1 - 1991/12/24
N2 - A fibre-optic biosensor based on the fluorimetric detection of NAD(P)H consumed or generated during enzymatic reactions is described. The design combines a miniaturized fluorimeter with a low-volume membrane reactor and utilizes flow-injection analysis principles. Two types of membrane reactor were developed and tested. In the first version, enzymes and a macromolecular nicotinamide adenine dinucleotide coenzyme derivative of polythyleneglycol, PEG-N6-(2-aminoethyl)-NAD(P)(H), are retained in the direct vicinity of the sensor tip. In the second version, a solid-supported liquid membrane was used to retain native coenzyme, while substrates and products were transported via selective carriers through an organic membrane barrier. The automated determination of phenylpyruvate, pyruvate, lactate, formate, ethanol and mannitol concentrations in model media was possible in the range of 50-1500 μmol l-1. The usefulness of the biosensor concept reported for real process monitoring in biotechnology or for medical purposes was demonstrated on different examples including the determination of ethanol for Saccharomyces cerevisiae cultivation.
AB - A fibre-optic biosensor based on the fluorimetric detection of NAD(P)H consumed or generated during enzymatic reactions is described. The design combines a miniaturized fluorimeter with a low-volume membrane reactor and utilizes flow-injection analysis principles. Two types of membrane reactor were developed and tested. In the first version, enzymes and a macromolecular nicotinamide adenine dinucleotide coenzyme derivative of polythyleneglycol, PEG-N6-(2-aminoethyl)-NAD(P)(H), are retained in the direct vicinity of the sensor tip. In the second version, a solid-supported liquid membrane was used to retain native coenzyme, while substrates and products were transported via selective carriers through an organic membrane barrier. The automated determination of phenylpyruvate, pyruvate, lactate, formate, ethanol and mannitol concentrations in model media was possible in the range of 50-1500 μmol l-1. The usefulness of the biosensor concept reported for real process monitoring in biotechnology or for medical purposes was demonstrated on different examples including the determination of ethanol for Saccharomyces cerevisiae cultivation.
KW - Biosensors
KW - Enzyme reactor
KW - Ethanol
KW - Fibre-optic sensor
KW - Fluorimetry
KW - Formate
KW - Glucose
KW - Lactate
KW - Mannitol
KW - Phenylpyruvate
KW - Pyruvate
UR - http://www.scopus.com/inward/record.url?scp=0026326636&partnerID=8YFLogxK
U2 - 10.1016/0003-2670(91)80050-4
DO - 10.1016/0003-2670(91)80050-4
M3 - Article
AN - SCOPUS:0026326636
VL - 255
SP - 223
EP - 229
JO - Analytica chimica acta
JF - Analytica chimica acta
SN - 0003-2670
IS - 2
ER -