Two fibre-optic sensors with confined enzymes and coenzymes: development and application

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  • Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)
  • Colorado State University
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OriginalspracheEnglisch
Seiten (von - bis)223-229
Seitenumfang7
FachzeitschriftAnalytica chimica acta
Jahrgang255
Ausgabenummer2
PublikationsstatusVeröffentlicht - 24 Dez. 1991

Abstract

A fibre-optic biosensor based on the fluorimetric detection of NAD(P)H consumed or generated during enzymatic reactions is described. The design combines a miniaturized fluorimeter with a low-volume membrane reactor and utilizes flow-injection analysis principles. Two types of membrane reactor were developed and tested. In the first version, enzymes and a macromolecular nicotinamide adenine dinucleotide coenzyme derivative of polythyleneglycol, PEG-N6-(2-aminoethyl)-NAD(P)(H), are retained in the direct vicinity of the sensor tip. In the second version, a solid-supported liquid membrane was used to retain native coenzyme, while substrates and products were transported via selective carriers through an organic membrane barrier. The automated determination of phenylpyruvate, pyruvate, lactate, formate, ethanol and mannitol concentrations in model media was possible in the range of 50-1500 μmol l-1. The usefulness of the biosensor concept reported for real process monitoring in biotechnology or for medical purposes was demonstrated on different examples including the determination of ethanol for Saccharomyces cerevisiae cultivation.

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Two fibre-optic sensors with confined enzymes and coenzymes: development and application. / Schelp, C.; Scheper, Th; Bückmann, F. et al.
in: Analytica chimica acta, Jahrgang 255, Nr. 2, 24.12.1991, S. 223-229.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Schelp C, Scheper T, Bückmann F, Reardon KF. Two fibre-optic sensors with confined enzymes and coenzymes: development and application. Analytica chimica acta. 1991 Dez 24;255(2):223-229. doi: 10.1016/0003-2670(91)80050-4
Schelp, C. ; Scheper, Th ; Bückmann, F. et al. / Two fibre-optic sensors with confined enzymes and coenzymes: development and application. in: Analytica chimica acta. 1991 ; Jahrgang 255, Nr. 2. S. 223-229.
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AU - Reardon, K. F.

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KW - Formate

KW - Glucose

KW - Lactate

KW - Mannitol

KW - Phenylpyruvate

KW - Pyruvate

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