TAL effector DNA-binding principles and specificity

Research output: Chapter in book/report/conference proceedingContribution to book/anthologyResearchpeer review

Authors

  • Annekatrin Richter
  • Jana Streubel
  • Jens Boch

External Research Organisations

  • Martin Luther University Halle-Wittenberg
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Details

Original languageEnglish
Title of host publicationTALENs
Subtitle of host publicationMethods and Protocols
Pages9-25
Number of pages17
Publication statusPublished - 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1338
ISSN (Print)1064-3745

Abstract

Transcription activator-like effectors (TALEs) are proteins with a unique DNA-binding domain that confers both a predictable and programmable specificity. The DNA-binding domain consists typically of 34-amino acid near-identical repeats. The repeats form a right-handed superhelical structure that wraps around the DNA double helix and exposes the variable amino acids at position 13 of each repeat to the sense strand DNA bases. Each repeat binds one base in a highly specific, non-overlapping, and comma-free fashion. Although TALE specificities are encoded in a simple way, sophisticated rules can be taken into account to build highly efficient DNA-binding modules for biotechnological use

Keywords

    CRISPR, Genome editing, Genome engineering, TALE, TALEN, Xanthomonas

ASJC Scopus subject areas

Cite this

TAL effector DNA-binding principles and specificity. / Richter, Annekatrin; Streubel, Jana; Boch, Jens.
TALENs: Methods and Protocols. 2016. p. 9-25 (Methods in Molecular Biology; Vol. 1338).

Research output: Chapter in book/report/conference proceedingContribution to book/anthologyResearchpeer review

Richter, A, Streubel, J & Boch, J 2016, TAL effector DNA-binding principles and specificity. in TALENs: Methods and Protocols. Methods in Molecular Biology, vol. 1338, pp. 9-25. https://doi.org/10.1007/978-1-4939-2932-0_2
Richter, A., Streubel, J., & Boch, J. (2016). TAL effector DNA-binding principles and specificity. In TALENs: Methods and Protocols (pp. 9-25). (Methods in Molecular Biology; Vol. 1338). https://doi.org/10.1007/978-1-4939-2932-0_2
Richter A, Streubel J, Boch J. TAL effector DNA-binding principles and specificity. In TALENs: Methods and Protocols. 2016. p. 9-25. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-2932-0_2
Richter, Annekatrin ; Streubel, Jana ; Boch, Jens. / TAL effector DNA-binding principles and specificity. TALENs: Methods and Protocols. 2016. pp. 9-25 (Methods in Molecular Biology).
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