TAL effector DNA-binding principles and specificity

Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Beitrag in Buch/Sammelwerk › Forschung › Peer-Review

Autoren

  • Annekatrin Richter
  • Jana Streubel
  • Jens Boch

Externe Organisationen

  • Martin-Luther-Universität Halle-Wittenberg
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Titel des SammelwerksTALENs
UntertitelMethods and Protocols
Seiten9-25
Seitenumfang17
PublikationsstatusVeröffentlicht - 2016
Extern publiziertJa

Publikationsreihe

NameMethods in Molecular Biology
Band1338
ISSN (Print)1064-3745

Abstract

Transcription activator-like effectors (TALEs) are proteins with a unique DNA-binding domain that confers both a predictable and programmable specificity. The DNA-binding domain consists typically of 34-amino acid near-identical repeats. The repeats form a right-handed superhelical structure that wraps around the DNA double helix and exposes the variable amino acids at position 13 of each repeat to the sense strand DNA bases. Each repeat binds one base in a highly specific, non-overlapping, and comma-free fashion. Although TALE specificities are encoded in a simple way, sophisticated rules can be taken into account to build highly efficient DNA-binding modules for biotechnological use

ASJC Scopus Sachgebiete

  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Molekularbiologie
  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Genetik

Zitieren

TAL effector DNA-binding principles and specificity. / Richter, Annekatrin; Streubel, Jana; Boch, Jens.
TALENs: Methods and Protocols. 2016. S. 9-25 (Methods in Molecular Biology; Band 1338).

Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Beitrag in Buch/Sammelwerk › Forschung › Peer-Review

Richter, A, Streubel, J & Boch, J 2016, TAL effector DNA-binding principles and specificity. in TALENs: Methods and Protocols. Methods in Molecular Biology, Bd. 1338, S. 9-25. https://doi.org/10.1007/978-1-4939-2932-0_2
Richter, A., Streubel, J., & Boch, J. (2016). TAL effector DNA-binding principles and specificity. In TALENs: Methods and Protocols (S. 9-25). (Methods in Molecular Biology; Band 1338). https://doi.org/10.1007/978-1-4939-2932-0_2
Richter A, Streubel J, Boch J. TAL effector DNA-binding principles and specificity. in TALENs: Methods and Protocols. 2016. S. 9-25. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-2932-0_2
Richter, Annekatrin ; Streubel, Jana ; Boch, Jens. / TAL effector DNA-binding principles and specificity. TALENs: Methods and Protocols. 2016. S. 9-25 (Methods in Molecular Biology).
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