TY - JOUR

T1 - Potential of electrospun cationic BSA fibers to guide osteogenic MSC differentiation via surface charge and fibrous topography

AU - Raic, Annamarija

AU - Friedrich, Frank

AU - Kratzer, Domenic

AU - Bieback, Karen

AU - Lahann, Joerg

AU - Lee-Thedieck, Cornelia

N1 - Funding Information: We thank Katharina Brändle (Karlsruhe Institute of Technology, Germany; Leibniz University Hannover, Germany) for RT-qPCR analysis and Chandralekha Chatterjee (Leibniz University Hannover, Germany) for proofreading the manuscript. Furthermore, we thank Dr. Dr. Michael Hirtz (Karlsruhe Institute of Technology, Institute for Nanotechnology) for his outstanding assistance on the AFM measurements and we thank Dr. Julia Hümmer (Karlsruhe Institute of Technology, Germany; Leibniz University Hannover, Germany) for repeating the fiber degradation experiment. The project was supported by contract research ‘Bioinspirierte Materialsynthese’ of the Baden-Württemberg Foundation (BioMatS-14) and by the BMBF NanoMatFutur Program (FKZ 13N12968 and 13XP5076A). C. L.-T. acknowledges support from the framework of the SMART BIOTECS alliance between the Technische Universität Braunschweig and the Leibniz Universität Hannover. This initiative is supported by the Ministry of Science and Culture (MWK) of Lower Saxony, Germany. C. L.-T. has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 757490). The publication of this article was funded by the Open Access Fund of the Leibniz Universität Hannover.

PY - 2019/12/27

Y1 - 2019/12/27

N2 - Large or complex bone fractures often need clinical treatments for sufficient bone repair. New treatment strategies have pursued the idea of using mesenchymal stromal cells (MSCs) in combination with osteoinductive materials to guide differentiation of MSCs into bone cells ensuring complete bone regeneration. To overcome the challenge of developing such materials, fundamental studies are needed to analyze and understand the MSC behavior on modified surfaces of applicable materials for bone healing. For this purpose, we developed a fibrous scaffold resembling the bone/bone marrow extracellular matrix (ECM) based on protein without addition of synthetic polymers. With this biomimetic in vitro model we identified the fibrous structure as well as the charge of the material to be responsible for its effects on MSC differentiation. Positive charge was introduced via cationization that additionally supported the stability of the scaffold in cell culture, and acted as nucleation point for mineralization during osteogenesis. Furthermore, we revealed enhanced focal adhesion formation and osteogenic differentiation of MSCs cultured on positively charged protein fibers. This pure protein-based and chemically modifiable, fibrous ECM model allows the investigation of MSC behavior on biomimetic materials to unfold new vistas how to direct cells’ differentiation for the development of new bone regenerating strategies.

AB - Large or complex bone fractures often need clinical treatments for sufficient bone repair. New treatment strategies have pursued the idea of using mesenchymal stromal cells (MSCs) in combination with osteoinductive materials to guide differentiation of MSCs into bone cells ensuring complete bone regeneration. To overcome the challenge of developing such materials, fundamental studies are needed to analyze and understand the MSC behavior on modified surfaces of applicable materials for bone healing. For this purpose, we developed a fibrous scaffold resembling the bone/bone marrow extracellular matrix (ECM) based on protein without addition of synthetic polymers. With this biomimetic in vitro model we identified the fibrous structure as well as the charge of the material to be responsible for its effects on MSC differentiation. Positive charge was introduced via cationization that additionally supported the stability of the scaffold in cell culture, and acted as nucleation point for mineralization during osteogenesis. Furthermore, we revealed enhanced focal adhesion formation and osteogenic differentiation of MSCs cultured on positively charged protein fibers. This pure protein-based and chemically modifiable, fibrous ECM model allows the investigation of MSC behavior on biomimetic materials to unfold new vistas how to direct cells’ differentiation for the development of new bone regenerating strategies.

UR - http://www.scopus.com/inward/record.url?scp=85077147840&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-56508-6

DO - 10.1038/s41598-019-56508-6

M3 - Article

VL - 9

JO - Scientific reports

JF - Scientific reports

SN - 2045-2322

IS - 1

M1 - 20003

ER -