Details
| Original language | English |
|---|---|
| Title of host publication | Frontiers in Ultrafast Optics |
| Subtitle of host publication | Biomedical, Scientific, and Industrial Applications XIV |
| Publisher | SPIE |
| ISBN (print) | 9780819498854 |
| Publication status | Published - 7 Mar 2014 |
| Externally published | Yes |
| Event | Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV - San Francisco, CA, United States Duration: 2 Feb 2014 → 5 Feb 2014 |
Publication series
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Volume | 8972 |
| ISSN (Print) | 0277-786X |
| ISSN (electronic) | 1996-756X |
Abstract
The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output.
Keywords
- Bessel beams, Optoinjection, Photoporation, Plant cells, Pulse dispersion compensation, Ultrafast optics
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Condensed Matter Physics
- Computer Science(all)
- Computer Science Applications
- Mathematics(all)
- Applied Mathematics
- Engineering(all)
- Electrical and Electronic Engineering
Cite this
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- BibTeX
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Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV. SPIE, 2014. 89720C (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8972).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Femtosecond optical injection of intact plant cells using a reconfigurable platform
AU - Mitchell, Claire A.
AU - Kalies, Stefan
AU - Cizmar, Tomas
AU - Bellini, Nicola
AU - Kubasik-Thayil, Anisha
AU - Heisterkamp, Alexander
AU - Torrance, Lesley
AU - Roberts, Alison G.
AU - Gunn-Moore, Frank J.
AU - Dholakia, Kishan
PY - 2014/3/7
Y1 - 2014/3/7
N2 - The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output.
AB - The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output.
KW - Bessel beams
KW - Optoinjection
KW - Photoporation
KW - Plant cells
KW - Pulse dispersion compensation
KW - Ultrafast optics
UR - http://www.scopus.com/inward/record.url?scp=84900477481&partnerID=8YFLogxK
U2 - 10.1117/12.2037784
DO - 10.1117/12.2037784
M3 - Conference contribution
AN - SCOPUS:84900477481
SN - 9780819498854
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Frontiers in Ultrafast Optics
PB - SPIE
T2 - Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV
Y2 - 2 February 2014 through 5 February 2014
ER -