Loading [MathJax]/extensions/tex2jax.js

Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin

Research output: Contribution to journal › Article › Research › peer review

Authors

  • A. I. Shpichka
  • A. V. Koroleva
  • A. Deiwick
  • P. S. Timashev
  • B. N. Chichkov

External Research Organisations

  • Penza State University
  • Laser Zentrum Hannover e.V. (LZH)
  • Institute of Photon Technologies of Federal Scientific Research Centre “Crystallography and Photonics” of the Russian Academy of Sciences
  • Sechenov First Moscow State Medical University
  • Federal Scientific Clinical Center of Specialized Types of Medical Aid and Medical Technologies of Federal Medical Biological Agency
Plum Print visual indicator of research metrics
  • Citations
    • Citation Indexes: 14
  • Captures
    • Readers: 21
see details

Details

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalCell and Tissue Biology
Volume11
Issue number1
Publication statusPublished - 16 Feb 2017
Externally publishedYes

Abstract

The engineering of blood vessels that could ensure efficient transport of various nutrients and metabolites is a challenge in tissue engineering. The creation of cell-seeded bioconstructs using modified natural polymers, in particular, PEGylated fibrin is under investigation, which will help overcome this problem. Therefore, the purpose of this study was to determine the optimal ratio of the hydrogel components of modified fibrin to provide favorable conditions for the vascular development of endothelial and mesenchymal stem cell coculture. We have shown that PEGylated fibrin gels are capable of maintaining three-dimensional growth of HUVEC and hASC cells. Hydrogel with a filamentous microporous structure obtained from PEGylated 5: 1 fibrinogen and thrombin at a concentration of 0.2 U per 1 mg ensured optimal conditions for spreading, growth, and development of cocultured cells as well as the expression of proteins involved in angiogenesis.

Keywords

    hydrogel, PEGylated fibrin, three-dimensional cell coculturing, tissue engineering, vasculogenesis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin. / Shpichka, A. I.; Koroleva, A. V.; Deiwick, A. et al.
In: Cell and Tissue Biology, Vol. 11, No. 1, 16.02.2017, p. 81-87.

Research output: Contribution to journal › Article › Research › peer review

Shpichka, AI, Koroleva, AV, Deiwick, A, Timashev, PS, Semenova, EF, Moiseeva, IY, Konoplyannikov, MA & Chichkov, BN 2017, 'Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin', Cell and Tissue Biology, vol. 11, no. 1, pp. 81-87. https://doi.org/10.1134/s1990519x17010126
Shpichka, A. I., Koroleva, A. V., Deiwick, A., Timashev, P. S., Semenova, E. F., Moiseeva, I. Y., Konoplyannikov, M. A., & Chichkov, B. N. (2017). Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin. Cell and Tissue Biology, 11(1), 81-87. https://doi.org/10.1134/s1990519x17010126
Shpichka AI, Koroleva AV, Deiwick A, Timashev PS, Semenova EF, Moiseeva IY et al. Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin. Cell and Tissue Biology. 2017 Feb 16;11(1):81-87. doi: 10.1134/s1990519x17010126
Shpichka, A. I. ; Koroleva, A. V. ; Deiwick, A. et al. / Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin. In: Cell and Tissue Biology. 2017 ; Vol. 11, No. 1. pp. 81-87.
Download
@article{9e0dccf3defa4c35a302b4acb51cfd63,
title = "Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin",
abstract = "The engineering of blood vessels that could ensure efficient transport of various nutrients and metabolites is a challenge in tissue engineering. The creation of cell-seeded bioconstructs using modified natural polymers, in particular, PEGylated fibrin is under investigation, which will help overcome this problem. Therefore, the purpose of this study was to determine the optimal ratio of the hydrogel components of modified fibrin to provide favorable conditions for the vascular development of endothelial and mesenchymal stem cell coculture. We have shown that PEGylated fibrin gels are capable of maintaining three-dimensional growth of HUVEC and hASC cells. Hydrogel with a filamentous microporous structure obtained from PEGylated 5: 1 fibrinogen and thrombin at a concentration of 0.2 U per 1 mg ensured optimal conditions for spreading, growth, and development of cocultured cells as well as the expression of proteins involved in angiogenesis.",
keywords = "hydrogel, PEGylated fibrin, three-dimensional cell coculturing, tissue engineering, vasculogenesis",
author = "Shpichka, {A. I.} and Koroleva, {A. V.} and A. Deiwick and Timashev, {P. S.} and Semenova, {E. F.} and Moiseeva, {I. Ya} and Konoplyannikov, {M. A.} and Chichkov, {B. N.}",
year = "2017",
month = feb,
day = "16",
doi = "10.1134/s1990519x17010126",
language = "English",
volume = "11",
pages = "81--87",
number = "1",

}

Download

TY - JOUR

T1 - Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin

AU - Shpichka, A. I.

AU - Koroleva, A. V.

AU - Deiwick, A.

AU - Timashev, P. S.

AU - Semenova, E. F.

AU - Moiseeva, I. Ya

AU - Konoplyannikov, M. A.

AU - Chichkov, B. N.

PY - 2017/2/16

Y1 - 2017/2/16

N2 - The engineering of blood vessels that could ensure efficient transport of various nutrients and metabolites is a challenge in tissue engineering. The creation of cell-seeded bioconstructs using modified natural polymers, in particular, PEGylated fibrin is under investigation, which will help overcome this problem. Therefore, the purpose of this study was to determine the optimal ratio of the hydrogel components of modified fibrin to provide favorable conditions for the vascular development of endothelial and mesenchymal stem cell coculture. We have shown that PEGylated fibrin gels are capable of maintaining three-dimensional growth of HUVEC and hASC cells. Hydrogel with a filamentous microporous structure obtained from PEGylated 5: 1 fibrinogen and thrombin at a concentration of 0.2 U per 1 mg ensured optimal conditions for spreading, growth, and development of cocultured cells as well as the expression of proteins involved in angiogenesis.

AB - The engineering of blood vessels that could ensure efficient transport of various nutrients and metabolites is a challenge in tissue engineering. The creation of cell-seeded bioconstructs using modified natural polymers, in particular, PEGylated fibrin is under investigation, which will help overcome this problem. Therefore, the purpose of this study was to determine the optimal ratio of the hydrogel components of modified fibrin to provide favorable conditions for the vascular development of endothelial and mesenchymal stem cell coculture. We have shown that PEGylated fibrin gels are capable of maintaining three-dimensional growth of HUVEC and hASC cells. Hydrogel with a filamentous microporous structure obtained from PEGylated 5: 1 fibrinogen and thrombin at a concentration of 0.2 U per 1 mg ensured optimal conditions for spreading, growth, and development of cocultured cells as well as the expression of proteins involved in angiogenesis.

KW - hydrogel

KW - PEGylated fibrin

KW - three-dimensional cell coculturing

KW - tissue engineering

KW - vasculogenesis

UR - http://www.scopus.com/inward/record.url?scp=85013001211&partnerID=8YFLogxK

U2 - 10.1134/s1990519x17010126

DO - 10.1134/s1990519x17010126

M3 - Article

AN - SCOPUS:85013001211

VL - 11

SP - 81

EP - 87

JO - Cell and Tissue Biology

JF - Cell and Tissue Biology

SN - 1990-519X

IS - 1

ER -