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Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autorschaft

  • A. I. Shpichka
  • A. V. Koroleva
  • A. Deiwick
  • P. S. Timashev
  • B. N. Chichkov

Externe Organisationen

  • Penza State University
  • Laser Zentrum Hannover e.V. (LZH)
  • Institute of Photon Technologies of Federal Scientific Research Centre “Crystallography and Photonics” of the Russian Academy of Sciences
  • Sechenov First Moscow State Medical University
  • Federal Scientific Clinical Center of Specialized Types of Medical Aid and Medical Technologies of Federal Medical Biological Agency

Details

OriginalspracheEnglisch
Seiten (von - bis)81-87
Seitenumfang7
FachzeitschriftCell and Tissue Biology
Jahrgang11
Ausgabenummer1
PublikationsstatusVeröffentlicht - 16 Feb. 2017
Extern publiziertJa

Abstract

The engineering of blood vessels that could ensure efficient transport of various nutrients and metabolites is a challenge in tissue engineering. The creation of cell-seeded bioconstructs using modified natural polymers, in particular, PEGylated fibrin is under investigation, which will help overcome this problem. Therefore, the purpose of this study was to determine the optimal ratio of the hydrogel components of modified fibrin to provide favorable conditions for the vascular development of endothelial and mesenchymal stem cell coculture. We have shown that PEGylated fibrin gels are capable of maintaining three-dimensional growth of HUVEC and hASC cells. Hydrogel with a filamentous microporous structure obtained from PEGylated 5: 1 fibrinogen and thrombin at a concentration of 0.2 U per 1 mg ensured optimal conditions for spreading, growth, and development of cocultured cells as well as the expression of proteins involved in angiogenesis.

ASJC Scopus Sachgebiete

  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Zellbiologie

Zitieren

Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin. / Shpichka, A. I.; Koroleva, A. V.; Deiwick, A. et al.
in: Cell and Tissue Biology, Jahrgang 11, Nr. 1, 16.02.2017, S. 81-87.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Shpichka, AI, Koroleva, AV, Deiwick, A, Timashev, PS, Semenova, EF, Moiseeva, IY, Konoplyannikov, MA & Chichkov, BN 2017, 'Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin', Cell and Tissue Biology, Jg. 11, Nr. 1, S. 81-87. https://doi.org/10.1134/s1990519x17010126
Shpichka, A. I., Koroleva, A. V., Deiwick, A., Timashev, P. S., Semenova, E. F., Moiseeva, I. Y., Konoplyannikov, M. A., & Chichkov, B. N. (2017). Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin. Cell and Tissue Biology, 11(1), 81-87. https://doi.org/10.1134/s1990519x17010126
Shpichka AI, Koroleva AV, Deiwick A, Timashev PS, Semenova EF, Moiseeva IY et al. Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin. Cell and Tissue Biology. 2017 Feb 16;11(1):81-87. doi: 10.1134/s1990519x17010126
Shpichka, A. I. ; Koroleva, A. V. ; Deiwick, A. et al. / Evaluation of the Vasculogenic Potential of Hydrogels Based on Modified Fibrin. in: Cell and Tissue Biology. 2017 ; Jahrgang 11, Nr. 1. S. 81-87.
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AU - Shpichka, A. I.

AU - Koroleva, A. V.

AU - Deiwick, A.

AU - Timashev, P. S.

AU - Semenova, E. F.

AU - Moiseeva, I. Ya

AU - Konoplyannikov, M. A.

AU - Chichkov, B. N.

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