Details
| Original language | English |
|---|---|
| Article number | 1350 |
| Journal | Frontiers in Plant Science |
| Volume | 10 |
| Publication status | Published - 30 Oct 2019 |
Abstract
The identification of novel herbicides is of crucial importance to modern agriculture. We developed an efficient in vivo assay based on oxygen evolution measurements using suspensions of chlorenchyma cells isolated from the single-cell C4 plant Bienertia sinuspersici to identify and characterize inhibitors of C4 photosynthesis. This novel approach fills the gap between conventional in vitro assays for inhibitors targeting C4 key enzymes and in vivo experiments on whole plants. The assay addresses inhibition of the target enzymes in a plant context thereby taking care of any reduced target inhibition due to metabolization or inadequate uptake of small molecule inhibitors across plant cell walls and membranes. Known small molecule inhibitors targeting C4 photosynthesis were used to validate the approach. To this end, we tested pyruvate phosphate dikinase inhibitor bisindolylmaleimide IV and phosphoenolpyruvate carboxylase inhibitor okanin. Both inhibitors show inhibition of plant photosynthesis at half-maximal inhibitory concentrations in the sub-mM range and confirm their potential to act as a new class of C4 selective inhibitors.
Keywords
- Bienertia sinuspersici, C photosynthesis, Inhibitor, cell suspension, phosphoenolpyruvate carboxylase, pyruvate phosphate dikinase, screening
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Frontiers in Plant Science, Vol. 10, 1350, 30.10.2019.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Efficient In Vivo Screening Method for the Identification of C4 Photosynthesis Inhibitors Based on Cell Suspensions of the Single-Cell C4 Plant Bienertia sinuspersici
AU - Minges, Alexander
AU - Janßen, Dominik
AU - Offermann, Sascha
AU - Groth, Georg
N1 - Funding Information: Computational support and infrastructure was provided by the Centre for Information and Media Technology (ZIM) at the University of Düsseldorf (Germany). Funding Information: Funded in part by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany´s Excellence Strategy — EXC 2048/1 — Project ID: 390686111. Work in the S.O. lab was funded by the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. 0109532016)”. Rural Development Administration, Republic of Korea.
PY - 2019/10/30
Y1 - 2019/10/30
N2 - The identification of novel herbicides is of crucial importance to modern agriculture. We developed an efficient in vivo assay based on oxygen evolution measurements using suspensions of chlorenchyma cells isolated from the single-cell C4 plant Bienertia sinuspersici to identify and characterize inhibitors of C4 photosynthesis. This novel approach fills the gap between conventional in vitro assays for inhibitors targeting C4 key enzymes and in vivo experiments on whole plants. The assay addresses inhibition of the target enzymes in a plant context thereby taking care of any reduced target inhibition due to metabolization or inadequate uptake of small molecule inhibitors across plant cell walls and membranes. Known small molecule inhibitors targeting C4 photosynthesis were used to validate the approach. To this end, we tested pyruvate phosphate dikinase inhibitor bisindolylmaleimide IV and phosphoenolpyruvate carboxylase inhibitor okanin. Both inhibitors show inhibition of plant photosynthesis at half-maximal inhibitory concentrations in the sub-mM range and confirm their potential to act as a new class of C4 selective inhibitors.
AB - The identification of novel herbicides is of crucial importance to modern agriculture. We developed an efficient in vivo assay based on oxygen evolution measurements using suspensions of chlorenchyma cells isolated from the single-cell C4 plant Bienertia sinuspersici to identify and characterize inhibitors of C4 photosynthesis. This novel approach fills the gap between conventional in vitro assays for inhibitors targeting C4 key enzymes and in vivo experiments on whole plants. The assay addresses inhibition of the target enzymes in a plant context thereby taking care of any reduced target inhibition due to metabolization or inadequate uptake of small molecule inhibitors across plant cell walls and membranes. Known small molecule inhibitors targeting C4 photosynthesis were used to validate the approach. To this end, we tested pyruvate phosphate dikinase inhibitor bisindolylmaleimide IV and phosphoenolpyruvate carboxylase inhibitor okanin. Both inhibitors show inhibition of plant photosynthesis at half-maximal inhibitory concentrations in the sub-mM range and confirm their potential to act as a new class of C4 selective inhibitors.
KW - Bienertia sinuspersici
KW - C photosynthesis
KW - Inhibitor
KW - cell suspension
KW - phosphoenolpyruvate carboxylase
KW - pyruvate phosphate dikinase
KW - screening
UR - http://www.scopus.com/inward/record.url?scp=85075236652&partnerID=8YFLogxK
U2 - 10.3389/fpls.2019.01350
DO - 10.3389/fpls.2019.01350
M3 - Article
C2 - 31736996
VL - 10
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 1350
ER -