Coupling methane oxidation and N2 fixation under methanogenic conditions in contrasting environments

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Authors

  • Yongliang Mo
  • Jiwei Li
  • Xiaotong Peng
  • Adrian Ho
  • Zhongjun Jia

Research Organisations

External Research Organisations

  • Chinese Academy of Sciences (CAS)
  • China West Normal University
  • Institute of Deep-Sea Science and Engineering
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Details

Original languageEnglish
Article number103693
Number of pages4
JournalEuropean journal of soil biology
Volume123
Early online date23 Nov 2024
Publication statusPublished - Dec 2024

Abstract

Microbial methane oxidation under widespread suboxic environment is crucial for understanding methane emission. However, the role of aerobic methanotrophs in mediating methane oxidation and nitrogen fixation is less understood in oxygen-limiting environments. In this study, we identified diazotrophic methanotrophs under oxygen-limited conditions (initial O2 of 6–8 μM) in two contrasting habitats (paddy soil and marine sediment) using DNA-based stable isotope probing combined with amplicon sequencing. Consistently, we documented significant 13CH4 oxidation and 15N2 fixation after 740 days of suboxic isotope labeling. Sequencing analysis revealed the predominance of Methylobacter–affiliated aerobic methanotrophs in the 13C-labeled DNA fractions. These Methylobacter-like OTUs accounted for 97.86 % in paddy soil and 99.49 % in marine sediment of the total pmoA gene sequences; while relative abundances for the nifH gene sequences were 91.59 % in paddy soil and 99.49 % in marine sediment. Taken together, our analyses revealed that Methylobacter is responsible for methane oxidation and nitrogen fixation under oxygen limitation in both habitats, demonstrating convergent emergence of this aerobic methanotroph under oxygen deficiency.

Keywords

    Marine sediment, Methane oxidation, Nitrogen fixation, Paddy soil, Suboxic conditions

ASJC Scopus subject areas

Sustainable Development Goals

Cite this

Coupling methane oxidation and N2 fixation under methanogenic conditions in contrasting environments. / Mo, Yongliang; Li, Jiwei; Peng, Xiaotong et al.
In: European journal of soil biology, Vol. 123, 103693, 12.2024.

Research output: Contribution to journalArticleResearchpeer review

Mo Y, Li J, Peng X, Ho A, Jia Z. Coupling methane oxidation and N2 fixation under methanogenic conditions in contrasting environments. European journal of soil biology. 2024 Dec;123:103693. Epub 2024 Nov 23. doi: 10.1016/j.ejsobi.2024.103693
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AU - Mo, Yongliang

AU - Li, Jiwei

AU - Peng, Xiaotong

AU - Ho, Adrian

AU - Jia, Zhongjun

N1 - Publisher Copyright: © 2024 Elsevier Masson SAS

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N2 - Microbial methane oxidation under widespread suboxic environment is crucial for understanding methane emission. However, the role of aerobic methanotrophs in mediating methane oxidation and nitrogen fixation is less understood in oxygen-limiting environments. In this study, we identified diazotrophic methanotrophs under oxygen-limited conditions (initial O2 of 6–8 μM) in two contrasting habitats (paddy soil and marine sediment) using DNA-based stable isotope probing combined with amplicon sequencing. Consistently, we documented significant 13CH4 oxidation and 15N2 fixation after 740 days of suboxic isotope labeling. Sequencing analysis revealed the predominance of Methylobacter–affiliated aerobic methanotrophs in the 13C-labeled DNA fractions. These Methylobacter-like OTUs accounted for 97.86 % in paddy soil and 99.49 % in marine sediment of the total pmoA gene sequences; while relative abundances for the nifH gene sequences were 91.59 % in paddy soil and 99.49 % in marine sediment. Taken together, our analyses revealed that Methylobacter is responsible for methane oxidation and nitrogen fixation under oxygen limitation in both habitats, demonstrating convergent emergence of this aerobic methanotroph under oxygen deficiency.

AB - Microbial methane oxidation under widespread suboxic environment is crucial for understanding methane emission. However, the role of aerobic methanotrophs in mediating methane oxidation and nitrogen fixation is less understood in oxygen-limiting environments. In this study, we identified diazotrophic methanotrophs under oxygen-limited conditions (initial O2 of 6–8 μM) in two contrasting habitats (paddy soil and marine sediment) using DNA-based stable isotope probing combined with amplicon sequencing. Consistently, we documented significant 13CH4 oxidation and 15N2 fixation after 740 days of suboxic isotope labeling. Sequencing analysis revealed the predominance of Methylobacter–affiliated aerobic methanotrophs in the 13C-labeled DNA fractions. These Methylobacter-like OTUs accounted for 97.86 % in paddy soil and 99.49 % in marine sediment of the total pmoA gene sequences; while relative abundances for the nifH gene sequences were 91.59 % in paddy soil and 99.49 % in marine sediment. Taken together, our analyses revealed that Methylobacter is responsible for methane oxidation and nitrogen fixation under oxygen limitation in both habitats, demonstrating convergent emergence of this aerobic methanotroph under oxygen deficiency.

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