Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 103693 |
| Seitenumfang | 4 |
| Fachzeitschrift | European journal of soil biology |
| Jahrgang | 123 |
| Frühes Online-Datum | 23 Nov. 2024 |
| Publikationsstatus | Veröffentlicht - Dez. 2024 |
Abstract
Microbial methane oxidation under widespread suboxic environment is crucial for understanding methane emission. However, the role of aerobic methanotrophs in mediating methane oxidation and nitrogen fixation is less understood in oxygen-limiting environments. In this study, we identified diazotrophic methanotrophs under oxygen-limited conditions (initial O2 of 6–8 μM) in two contrasting habitats (paddy soil and marine sediment) using DNA-based stable isotope probing combined with amplicon sequencing. Consistently, we documented significant 13CH4 oxidation and 15N2 fixation after 740 days of suboxic isotope labeling. Sequencing analysis revealed the predominance of Methylobacter–affiliated aerobic methanotrophs in the 13C-labeled DNA fractions. These Methylobacter-like OTUs accounted for 97.86 % in paddy soil and 99.49 % in marine sediment of the total pmoA gene sequences; while relative abundances for the nifH gene sequences were 91.59 % in paddy soil and 99.49 % in marine sediment. Taken together, our analyses revealed that Methylobacter is responsible for methane oxidation and nitrogen fixation under oxygen limitation in both habitats, demonstrating convergent emergence of this aerobic methanotroph under oxygen deficiency.
ASJC Scopus Sachgebiete
- Immunologie und Mikrobiologie (insg.)
- Mikrobiologie
- Agrar- und Biowissenschaften (insg.)
- Bodenkunde
- Agrar- und Biowissenschaften (insg.)
- Insektenkunde
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in: European journal of soil biology, Jahrgang 123, 103693, 12.2024.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Coupling methane oxidation and N2 fixation under methanogenic conditions in contrasting environments
AU - Mo, Yongliang
AU - Li, Jiwei
AU - Peng, Xiaotong
AU - Ho, Adrian
AU - Jia, Zhongjun
N1 - Publisher Copyright: © 2024 Elsevier Masson SAS
PY - 2024/12
Y1 - 2024/12
N2 - Microbial methane oxidation under widespread suboxic environment is crucial for understanding methane emission. However, the role of aerobic methanotrophs in mediating methane oxidation and nitrogen fixation is less understood in oxygen-limiting environments. In this study, we identified diazotrophic methanotrophs under oxygen-limited conditions (initial O2 of 6–8 μM) in two contrasting habitats (paddy soil and marine sediment) using DNA-based stable isotope probing combined with amplicon sequencing. Consistently, we documented significant 13CH4 oxidation and 15N2 fixation after 740 days of suboxic isotope labeling. Sequencing analysis revealed the predominance of Methylobacter–affiliated aerobic methanotrophs in the 13C-labeled DNA fractions. These Methylobacter-like OTUs accounted for 97.86 % in paddy soil and 99.49 % in marine sediment of the total pmoA gene sequences; while relative abundances for the nifH gene sequences were 91.59 % in paddy soil and 99.49 % in marine sediment. Taken together, our analyses revealed that Methylobacter is responsible for methane oxidation and nitrogen fixation under oxygen limitation in both habitats, demonstrating convergent emergence of this aerobic methanotroph under oxygen deficiency.
AB - Microbial methane oxidation under widespread suboxic environment is crucial for understanding methane emission. However, the role of aerobic methanotrophs in mediating methane oxidation and nitrogen fixation is less understood in oxygen-limiting environments. In this study, we identified diazotrophic methanotrophs under oxygen-limited conditions (initial O2 of 6–8 μM) in two contrasting habitats (paddy soil and marine sediment) using DNA-based stable isotope probing combined with amplicon sequencing. Consistently, we documented significant 13CH4 oxidation and 15N2 fixation after 740 days of suboxic isotope labeling. Sequencing analysis revealed the predominance of Methylobacter–affiliated aerobic methanotrophs in the 13C-labeled DNA fractions. These Methylobacter-like OTUs accounted for 97.86 % in paddy soil and 99.49 % in marine sediment of the total pmoA gene sequences; while relative abundances for the nifH gene sequences were 91.59 % in paddy soil and 99.49 % in marine sediment. Taken together, our analyses revealed that Methylobacter is responsible for methane oxidation and nitrogen fixation under oxygen limitation in both habitats, demonstrating convergent emergence of this aerobic methanotroph under oxygen deficiency.
KW - Marine sediment
KW - Methane oxidation
KW - Nitrogen fixation
KW - Paddy soil
KW - Suboxic conditions
UR - http://www.scopus.com/inward/record.url?scp=85209588145&partnerID=8YFLogxK
U2 - 10.1016/j.ejsobi.2024.103693
DO - 10.1016/j.ejsobi.2024.103693
M3 - Article
AN - SCOPUS:85209588145
VL - 123
JO - European journal of soil biology
JF - European journal of soil biology
SN - 1164-5563
M1 - 103693
ER -