Details
| Original language | English |
|---|---|
| Article number | 055001 |
| Journal | Journal of biomedical optics |
| Volume | 21 |
| Issue number | 5 |
| Publication status | Published - 9 May 2016 |
Abstract
The use of small particles has expanded the capability of ultrashort pulsed laser optoinjection technology toward simultaneous treatment of multiple cells. The microfluidic platform is one of the attractive systems that has obtained synergy with laser-based technology for cell manipulation, including optoinjection. We have demonstrated the delivery of molecules into suspended-flowing cells in a microfluidic channel by using biodegradable polymer microspheres and a near-infrared femtosecond laser pulse. The use of polylactic-co-glycolic acid microspheres realized not only a higher optoinjection ratio compared to that with polylactic acid microspheres but also avoids optical damage to the microfluidic chip, which is attributable to its higher optical intensity enhancement at the localized spot under a microsphere. Interestingly, optoinjection ratios to nucleus showed a difference for adhered cells and suspended cells. The use of biodegradable polymer microspheres provides high throughput optoinjection; i.e., multiple cells can be treated in a short time, which is promising for various applications in cell analysis, drug delivery, and ex vivo gene transfection to bone marrow cells and stem cells without concerns about residual microspheres.
Keywords
- Biodegradable polymer, Femtosecond laser, Laser cell perforation, Microfluidics, Optoinjection
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Engineering(all)
- Biomedical Engineering
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Journal of biomedical optics, Vol. 21, No. 5, 055001, 09.05.2016.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Biodegradable microsphere-mediated cell perforation in microfluidic channel using femtosecond laser
AU - Ishii, Atsuhiro
AU - Ariyasu, Kazumasa
AU - Mitsuhashi, Tatsuki
AU - Heinemann, Dag
AU - Heisterkamp, Alexander
AU - Terakawa, Mitsuhiro
PY - 2016/5/9
Y1 - 2016/5/9
N2 - The use of small particles has expanded the capability of ultrashort pulsed laser optoinjection technology toward simultaneous treatment of multiple cells. The microfluidic platform is one of the attractive systems that has obtained synergy with laser-based technology for cell manipulation, including optoinjection. We have demonstrated the delivery of molecules into suspended-flowing cells in a microfluidic channel by using biodegradable polymer microspheres and a near-infrared femtosecond laser pulse. The use of polylactic-co-glycolic acid microspheres realized not only a higher optoinjection ratio compared to that with polylactic acid microspheres but also avoids optical damage to the microfluidic chip, which is attributable to its higher optical intensity enhancement at the localized spot under a microsphere. Interestingly, optoinjection ratios to nucleus showed a difference for adhered cells and suspended cells. The use of biodegradable polymer microspheres provides high throughput optoinjection; i.e., multiple cells can be treated in a short time, which is promising for various applications in cell analysis, drug delivery, and ex vivo gene transfection to bone marrow cells and stem cells without concerns about residual microspheres.
AB - The use of small particles has expanded the capability of ultrashort pulsed laser optoinjection technology toward simultaneous treatment of multiple cells. The microfluidic platform is one of the attractive systems that has obtained synergy with laser-based technology for cell manipulation, including optoinjection. We have demonstrated the delivery of molecules into suspended-flowing cells in a microfluidic channel by using biodegradable polymer microspheres and a near-infrared femtosecond laser pulse. The use of polylactic-co-glycolic acid microspheres realized not only a higher optoinjection ratio compared to that with polylactic acid microspheres but also avoids optical damage to the microfluidic chip, which is attributable to its higher optical intensity enhancement at the localized spot under a microsphere. Interestingly, optoinjection ratios to nucleus showed a difference for adhered cells and suspended cells. The use of biodegradable polymer microspheres provides high throughput optoinjection; i.e., multiple cells can be treated in a short time, which is promising for various applications in cell analysis, drug delivery, and ex vivo gene transfection to bone marrow cells and stem cells without concerns about residual microspheres.
KW - Biodegradable polymer
KW - Femtosecond laser
KW - Laser cell perforation
KW - Microfluidics
KW - Optoinjection
UR - http://www.scopus.com/inward/record.url?scp=84977504057&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.21.5.055001
DO - 10.1117/1.JBO.21.5.055001
M3 - Article
C2 - 27156714
AN - SCOPUS:84977504057
VL - 21
JO - Journal of biomedical optics
JF - Journal of biomedical optics
SN - 1083-3668
IS - 5
M1 - 055001
ER -