Details
| Original language | English |
|---|---|
| Article number | 1930 |
| Journal | Plants |
| Volume | 12 |
| Issue number | 10 |
| Publication status | Published - 9 May 2023 |
Abstract
Barley (Hordeum vulgare) is one of the most widely cultivated crops for feedstock and beer production, whereas lupins (Lupinus spp.) are grown as fodder and their seeds are a source of protein. Both species produce the allelopathic alkaloids gramine and hordenine. These plant-specialized metabolites may be of economic interest for crop protection, depending on their tissue distribution. However, in high concentrations they pose a health risk to humans and animals that feed on them. This study was carried out to develop and validate a new method for monitoring these alkaloids and their related metabolites using fluorescence detection. Separation was performed on an HSS T3 column using slightly acidified water-acetonitrile eluents. Calibration plots expressed linearity over the range 0.09–100 pmol/µL for gramine. The accuracy and precision ranged from 97.8 to 123.4%, <7% RSD. The method was successfully applied in a study of the natural range of abundance of gramine, hordenine and their related metabolites, AMI, tryptophan and tyramine, in 22 barley accessions and 10 lupin species. This method provides accurate and highly sensitive chromatographic separation and detection of tryptophan- and tyrosine-derived allelochemicals and is an accessible alternative to LC-MS techniques for routine screening.
Keywords
- allelopathy, barley, chromatography, fluorescence detection, gramine, hordenine, lupin, UPLC
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Environmental Science(all)
- Ecology
- Agricultural and Biological Sciences(all)
- Plant Science
Sustainable Development Goals
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In: Plants, Vol. 12, No. 10, 1930, 09.05.2023.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - A New Fluorescence Detection Method for Tryptophan- and Tyrosine-Derived Allelopathic Compounds in Barley and Lupin
AU - Leite Dias, Sara
AU - Garibay-Hernández, Adriana
AU - Brendel, Fabian Leon
AU - Gabriel Chavez, Benjamin
AU - Brückner, Elena
AU - Mock, Hans Peter
AU - Franke, Jakob
AU - D’Auria, John Charles
N1 - Funding Information: We would like to thank the International Max-Planck Research School for support to S.L.D. and the Leibniz Research Alliance “Bioactive Compounds and Biotechnology” for a seed money grant to J.C.D.
PY - 2023/5/9
Y1 - 2023/5/9
N2 - Barley (Hordeum vulgare) is one of the most widely cultivated crops for feedstock and beer production, whereas lupins (Lupinus spp.) are grown as fodder and their seeds are a source of protein. Both species produce the allelopathic alkaloids gramine and hordenine. These plant-specialized metabolites may be of economic interest for crop protection, depending on their tissue distribution. However, in high concentrations they pose a health risk to humans and animals that feed on them. This study was carried out to develop and validate a new method for monitoring these alkaloids and their related metabolites using fluorescence detection. Separation was performed on an HSS T3 column using slightly acidified water-acetonitrile eluents. Calibration plots expressed linearity over the range 0.09–100 pmol/µL for gramine. The accuracy and precision ranged from 97.8 to 123.4%, <7% RSD. The method was successfully applied in a study of the natural range of abundance of gramine, hordenine and their related metabolites, AMI, tryptophan and tyramine, in 22 barley accessions and 10 lupin species. This method provides accurate and highly sensitive chromatographic separation and detection of tryptophan- and tyrosine-derived allelochemicals and is an accessible alternative to LC-MS techniques for routine screening.
AB - Barley (Hordeum vulgare) is one of the most widely cultivated crops for feedstock and beer production, whereas lupins (Lupinus spp.) are grown as fodder and their seeds are a source of protein. Both species produce the allelopathic alkaloids gramine and hordenine. These plant-specialized metabolites may be of economic interest for crop protection, depending on their tissue distribution. However, in high concentrations they pose a health risk to humans and animals that feed on them. This study was carried out to develop and validate a new method for monitoring these alkaloids and their related metabolites using fluorescence detection. Separation was performed on an HSS T3 column using slightly acidified water-acetonitrile eluents. Calibration plots expressed linearity over the range 0.09–100 pmol/µL for gramine. The accuracy and precision ranged from 97.8 to 123.4%, <7% RSD. The method was successfully applied in a study of the natural range of abundance of gramine, hordenine and their related metabolites, AMI, tryptophan and tyramine, in 22 barley accessions and 10 lupin species. This method provides accurate and highly sensitive chromatographic separation and detection of tryptophan- and tyrosine-derived allelochemicals and is an accessible alternative to LC-MS techniques for routine screening.
KW - allelopathy
KW - barley
KW - chromatography
KW - fluorescence detection
KW - gramine
KW - hordenine
KW - lupin
KW - UPLC
UR - http://www.scopus.com/inward/record.url?scp=85160555740&partnerID=8YFLogxK
U2 - 10.3390/plants12101930
DO - 10.3390/plants12101930
M3 - Article
AN - SCOPUS:85160555740
VL - 12
JO - Plants
JF - Plants
SN - 2223-7747
IS - 10
M1 - 1930
ER -