Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1229-1236 |
| Seitenumfang | 8 |
| Fachzeitschrift | Biosensors and Bioelectronics |
| Jahrgang | 11 |
| Ausgabenummer | 12 |
| Publikationsstatus | Veröffentlicht - 1996 |
Abstract
New reloadable biosensors were developed by affinity binding of glucoenzymes peroxidase and glucose oxidase on pF-sensitive field-effect transistors (Si/SiO2/Si3N4/LaF3 layers). The basic measuring principle of these ion sensitive field-effect transistors is the current change depending on the concentration of F- ions. The immobilized glucoenzymes can be removed from the enzymatically inactive Concanavalin A basic membrane and fresh enzymes can be bound again. The principle of this reloadable biosensor is described in detail. Additionally, the biosensor was successfully integrated in a flow-injection analysis system for measuring glucose during a cultivation process.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biophysik
- Ingenieurwesen (insg.)
- Biomedizintechnik
- Chemie (insg.)
- Elektrochemie
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in: Biosensors and Bioelectronics, Jahrgang 11, Nr. 12, 1996, S. 1229-1236.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Reversible coupling of glucoenzymes on fluoride-sensitive FET biosensors based on lectin-glucoprotein binding
AU - Köneke, R.
AU - Menzel, C.
AU - Ulber, R.
AU - Schügerl, K.
AU - Scheper, T.
AU - Saleemuddin, M.
PY - 1996
Y1 - 1996
N2 - New reloadable biosensors were developed by affinity binding of glucoenzymes peroxidase and glucose oxidase on pF-sensitive field-effect transistors (Si/SiO2/Si3N4/LaF3 layers). The basic measuring principle of these ion sensitive field-effect transistors is the current change depending on the concentration of F- ions. The immobilized glucoenzymes can be removed from the enzymatically inactive Concanavalin A basic membrane and fresh enzymes can be bound again. The principle of this reloadable biosensor is described in detail. Additionally, the biosensor was successfully integrated in a flow-injection analysis system for measuring glucose during a cultivation process.
AB - New reloadable biosensors were developed by affinity binding of glucoenzymes peroxidase and glucose oxidase on pF-sensitive field-effect transistors (Si/SiO2/Si3N4/LaF3 layers). The basic measuring principle of these ion sensitive field-effect transistors is the current change depending on the concentration of F- ions. The immobilized glucoenzymes can be removed from the enzymatically inactive Concanavalin A basic membrane and fresh enzymes can be bound again. The principle of this reloadable biosensor is described in detail. Additionally, the biosensor was successfully integrated in a flow-injection analysis system for measuring glucose during a cultivation process.
KW - Biosensor
KW - Concanavalin A
KW - Cultivation
KW - Field-effect transistor
KW - Flow-injection analysis
KW - Glucoenzyme
KW - Glucose
KW - Glucose oxidase
KW - Horseradish peroxidase
KW - Immobilization
KW - Lectin
UR - http://www.scopus.com/inward/record.url?scp=0029826815&partnerID=8YFLogxK
U2 - 10.1016/0956-5663(96)88088-2
DO - 10.1016/0956-5663(96)88088-2
M3 - Article
AN - SCOPUS:0029826815
VL - 11
SP - 1229
EP - 1236
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 12
ER -