Regarding the Influence of Additives and Additional Plasma-Induced Chemical Ionization on Adduct Formation in ESI/IMS/MS

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Christian Thoben
  • Nora T. Hartner
  • Moritz Hitzemann
  • Christian Robert Raddatz
  • Manuel Eckermann
  • Detlev Belder
  • Stefan Zimmermann

Externe Organisationen

  • Universität Leipzig
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)857-868
Seitenumfang12
FachzeitschriftJournal of the American Society for Mass Spectrometry
Jahrgang34
Ausgabenummer5
Frühes Online-Datum13 Apr. 2023
PublikationsstatusVeröffentlicht - 3 Mai 2023

Abstract

Ion mobility spectrometers (IMS) separate ions based on their ion mobility, which depends mainly on collision cross-section, mass, and charge of the ions. However, the performance is often hampered in electrospray ionization (ESI) by the appearance of multiple ion mobility peaks in the spectrum for the same analyte due to clustering and additional sodium adducts. In this work, we investigate the influence of solvents and buffer additives on the detected ion mobility peaks using ESI. Additionally, we investigate the effects of an additional chemical ionization (CI) induced by plasma ionization on the ions formed by electrospray. For this purpose, we coupled our high-resolution IMS with a resolving power of Rp = 100 to a time-of-flight mass spectrometer. Depending on the analyte and the chosen additives, the ionization process can be influenced during the electrospray process. For the herbicide isoproturon, the addition of 5 mM sodium acetate results in the formation of the sodium adduct [M + Na]+, which is reflected in the ion mobility K0 of 1.22 cm2/(V·s). In contrast, the addition of 5 mM ammonium acetate yields the protonated species [M + H]+ and a correspondingly higher K0 of 1.29 cm2/(V·s). In some cases, as with the herbicide pyrimethanil, the addition of sodium acetate can completely suppress ionizations. By carefully choosing the solvent additive for ESI-IMS or additional CI, the formation of different ion mobility peaks can be observed. This can facilitate the assignment of ions to ion mobility peaks using IMS as a compact, stand-alone instrument, e.g., for on-site analysis.

ASJC Scopus Sachgebiete

Zitieren

Regarding the Influence of Additives and Additional Plasma-Induced Chemical Ionization on Adduct Formation in ESI/IMS/MS. / Thoben, Christian; Hartner, Nora T.; Hitzemann, Moritz et al.
in: Journal of the American Society for Mass Spectrometry, Jahrgang 34, Nr. 5, 03.05.2023, S. 857-868.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Thoben C, Hartner NT, Hitzemann M, Raddatz CR, Eckermann M, Belder D et al. Regarding the Influence of Additives and Additional Plasma-Induced Chemical Ionization on Adduct Formation in ESI/IMS/MS. Journal of the American Society for Mass Spectrometry. 2023 Mai 3;34(5):857-868. Epub 2023 Apr 13. doi: 10.1021/jasms.2c00348
Download
@article{622b9c6933b741f6bf8c5d917f365f72,
title = "Regarding the Influence of Additives and Additional Plasma-Induced Chemical Ionization on Adduct Formation in ESI/IMS/MS",
abstract = "Ion mobility spectrometers (IMS) separate ions based on their ion mobility, which depends mainly on collision cross-section, mass, and charge of the ions. However, the performance is often hampered in electrospray ionization (ESI) by the appearance of multiple ion mobility peaks in the spectrum for the same analyte due to clustering and additional sodium adducts. In this work, we investigate the influence of solvents and buffer additives on the detected ion mobility peaks using ESI. Additionally, we investigate the effects of an additional chemical ionization (CI) induced by plasma ionization on the ions formed by electrospray. For this purpose, we coupled our high-resolution IMS with a resolving power of Rp = 100 to a time-of-flight mass spectrometer. Depending on the analyte and the chosen additives, the ionization process can be influenced during the electrospray process. For the herbicide isoproturon, the addition of 5 mM sodium acetate results in the formation of the sodium adduct [M + Na]+, which is reflected in the ion mobility K0 of 1.22 cm2/(V·s). In contrast, the addition of 5 mM ammonium acetate yields the protonated species [M + H]+ and a correspondingly higher K0 of 1.29 cm2/(V·s). In some cases, as with the herbicide pyrimethanil, the addition of sodium acetate can completely suppress ionizations. By carefully choosing the solvent additive for ESI-IMS or additional CI, the formation of different ion mobility peaks can be observed. This can facilitate the assignment of ions to ion mobility peaks using IMS as a compact, stand-alone instrument, e.g., for on-site analysis.",
keywords = "additives, DBD, dielectric-barrier discharge, electrospray ionization, IMS, IMS/MS, ion mobility spectrometry, mobile phase, pesticides, plasma source",
author = "Christian Thoben and Hartner, {Nora T.} and Moritz Hitzemann and Raddatz, {Christian Robert} and Manuel Eckermann and Detlev Belder and Stefan Zimmermann",
note = "Funding Information: Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – 396430937.",
year = "2023",
month = may,
day = "3",
doi = "10.1021/jasms.2c00348",
language = "English",
volume = "34",
pages = "857--868",
journal = "Journal of the American Society for Mass Spectrometry",
issn = "1044-0305",
publisher = "Springer New York",
number = "5",

}

Download

TY - JOUR

T1 - Regarding the Influence of Additives and Additional Plasma-Induced Chemical Ionization on Adduct Formation in ESI/IMS/MS

AU - Thoben, Christian

AU - Hartner, Nora T.

AU - Hitzemann, Moritz

AU - Raddatz, Christian Robert

AU - Eckermann, Manuel

AU - Belder, Detlev

AU - Zimmermann, Stefan

N1 - Funding Information: Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – 396430937.

PY - 2023/5/3

Y1 - 2023/5/3

N2 - Ion mobility spectrometers (IMS) separate ions based on their ion mobility, which depends mainly on collision cross-section, mass, and charge of the ions. However, the performance is often hampered in electrospray ionization (ESI) by the appearance of multiple ion mobility peaks in the spectrum for the same analyte due to clustering and additional sodium adducts. In this work, we investigate the influence of solvents and buffer additives on the detected ion mobility peaks using ESI. Additionally, we investigate the effects of an additional chemical ionization (CI) induced by plasma ionization on the ions formed by electrospray. For this purpose, we coupled our high-resolution IMS with a resolving power of Rp = 100 to a time-of-flight mass spectrometer. Depending on the analyte and the chosen additives, the ionization process can be influenced during the electrospray process. For the herbicide isoproturon, the addition of 5 mM sodium acetate results in the formation of the sodium adduct [M + Na]+, which is reflected in the ion mobility K0 of 1.22 cm2/(V·s). In contrast, the addition of 5 mM ammonium acetate yields the protonated species [M + H]+ and a correspondingly higher K0 of 1.29 cm2/(V·s). In some cases, as with the herbicide pyrimethanil, the addition of sodium acetate can completely suppress ionizations. By carefully choosing the solvent additive for ESI-IMS or additional CI, the formation of different ion mobility peaks can be observed. This can facilitate the assignment of ions to ion mobility peaks using IMS as a compact, stand-alone instrument, e.g., for on-site analysis.

AB - Ion mobility spectrometers (IMS) separate ions based on their ion mobility, which depends mainly on collision cross-section, mass, and charge of the ions. However, the performance is often hampered in electrospray ionization (ESI) by the appearance of multiple ion mobility peaks in the spectrum for the same analyte due to clustering and additional sodium adducts. In this work, we investigate the influence of solvents and buffer additives on the detected ion mobility peaks using ESI. Additionally, we investigate the effects of an additional chemical ionization (CI) induced by plasma ionization on the ions formed by electrospray. For this purpose, we coupled our high-resolution IMS with a resolving power of Rp = 100 to a time-of-flight mass spectrometer. Depending on the analyte and the chosen additives, the ionization process can be influenced during the electrospray process. For the herbicide isoproturon, the addition of 5 mM sodium acetate results in the formation of the sodium adduct [M + Na]+, which is reflected in the ion mobility K0 of 1.22 cm2/(V·s). In contrast, the addition of 5 mM ammonium acetate yields the protonated species [M + H]+ and a correspondingly higher K0 of 1.29 cm2/(V·s). In some cases, as with the herbicide pyrimethanil, the addition of sodium acetate can completely suppress ionizations. By carefully choosing the solvent additive for ESI-IMS or additional CI, the formation of different ion mobility peaks can be observed. This can facilitate the assignment of ions to ion mobility peaks using IMS as a compact, stand-alone instrument, e.g., for on-site analysis.

KW - additives

KW - DBD

KW - dielectric-barrier discharge

KW - electrospray ionization

KW - IMS

KW - IMS/MS

KW - ion mobility spectrometry

KW - mobile phase

KW - pesticides

KW - plasma source

UR - http://www.scopus.com/inward/record.url?scp=85154067539&partnerID=8YFLogxK

U2 - 10.1021/jasms.2c00348

DO - 10.1021/jasms.2c00348

M3 - Article

C2 - 37052511

AN - SCOPUS:85154067539

VL - 34

SP - 857

EP - 868

JO - Journal of the American Society for Mass Spectrometry

JF - Journal of the American Society for Mass Spectrometry

SN - 1044-0305

IS - 5

ER -

Von denselben Autoren