Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 043110 |
| Seitenumfang | 9 |
| Fachzeitschrift | Review of Scientific Instruments |
| Jahrgang | 90 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - 10 Apr. 2019 |
Abstract
Raman spectroscopy has proven to be an effective tool for molecular analysis in different applications. In clinical diagnostics, its application has enabled nondestructive investigation of biological tissues and liquids. The human perilymph, for example, is an inner ear liquid, essential for the hearing sensation. The composition of this liquid is correlated with pathophysiological parameters and was analyzed by extraction and mass spectrometry so far. In this work, we present a fiber optic probe setup for the Raman spectroscopic sampling of inner ear proteins in solution. Multivariate data analysis is applied for the discrimination of individual proteins (heat shock proteins) linked to a specific type of hearing impairment. This proof-of-principle is a first step toward a system for sensitive and continuous in vivo perilymph investigation in the future.
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- Physik und Astronomie (insg.)
- Instrumentierung
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in: Review of Scientific Instruments, Jahrgang 90, Nr. 4, 043110, 10.04.2019.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Multivariate discrimination of heat shock proteins using a fiber optic Raman setup for in situ analysis of human perilymph
AU - Höhl, Martin
AU - Zeilinger, Carsten
AU - Roth, Bernhard
AU - Meinhardt-Wollweber, Merve
AU - Morgner, Uwe
N1 - Funding information: The authors would like to thank Professor Waltraud Kessler for her support in multivariate data analysis. This work was supported by the DFG Cluster of Excellence, No. EXC 1077/1 “Hearing4all.”
PY - 2019/4/10
Y1 - 2019/4/10
N2 - Raman spectroscopy has proven to be an effective tool for molecular analysis in different applications. In clinical diagnostics, its application has enabled nondestructive investigation of biological tissues and liquids. The human perilymph, for example, is an inner ear liquid, essential for the hearing sensation. The composition of this liquid is correlated with pathophysiological parameters and was analyzed by extraction and mass spectrometry so far. In this work, we present a fiber optic probe setup for the Raman spectroscopic sampling of inner ear proteins in solution. Multivariate data analysis is applied for the discrimination of individual proteins (heat shock proteins) linked to a specific type of hearing impairment. This proof-of-principle is a first step toward a system for sensitive and continuous in vivo perilymph investigation in the future.
AB - Raman spectroscopy has proven to be an effective tool for molecular analysis in different applications. In clinical diagnostics, its application has enabled nondestructive investigation of biological tissues and liquids. The human perilymph, for example, is an inner ear liquid, essential for the hearing sensation. The composition of this liquid is correlated with pathophysiological parameters and was analyzed by extraction and mass spectrometry so far. In this work, we present a fiber optic probe setup for the Raman spectroscopic sampling of inner ear proteins in solution. Multivariate data analysis is applied for the discrimination of individual proteins (heat shock proteins) linked to a specific type of hearing impairment. This proof-of-principle is a first step toward a system for sensitive and continuous in vivo perilymph investigation in the future.
UR - http://www.scopus.com/inward/record.url?scp=85064194839&partnerID=8YFLogxK
U2 - 10.1063/1.5030301
DO - 10.1063/1.5030301
M3 - Article
C2 - 31043005
AN - SCOPUS:85064194839
VL - 90
JO - Review of Scientific Instruments
JF - Review of Scientific Instruments
SN - 0034-6748
IS - 4
M1 - 043110
ER -