Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 582-589 |
| Seitenumfang | 8 |
| Fachzeitschrift | Sensors and Actuators, B: Chemical |
| Jahrgang | 113 |
| Ausgabenummer | 2 |
| Publikationsstatus | Veröffentlicht - 11 Aug. 2005 |
Abstract
A highly sensitive combination of a membrane dipstick assay and a flatbed scanner for determination of botulinum toxin type D is described. The immunochromatographic assay is based on a sandwich format using two primary antibodies of distinct specificities and one secondary antibody. One of the primary antibodies was conjugated with colloidal gold (detector reagent), the secondary antibody (capture reagent) was immobilized within a defined detection zone (test line) on a diagnostic cellulose nitrate membrane. In combination with an effective sample pre-treatment, the gold conjugated antibody and the second distinct antibody formed a mobile sandwich complex with the toxin. Within the test line the mobile sandwich complex was immobilized and therefore concentrated by the secondary antibody resulting in a distinct red test line. The intensity of colour of the red test line (signal intensity), which correlated directly with the concentration of BoNT/D in the standard or spiked horse faecal sample, was assessed visually and by computer image analysis using a three-determination analysis. Thus, toxin concentrations down to 50 pg/mL were determined within 3.5 h. The mean relative error of the arithmetic mean of the signal intensity amounted to approximately 7%.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Elektronische, optische und magnetische Materialien
- Physik und Astronomie (insg.)
- Instrumentierung
- Physik und Astronomie (insg.)
- Physik der kondensierten Materie
- Werkstoffwissenschaften (insg.)
- Oberflächen, Beschichtungen und Folien
- Werkstoffwissenschaften (insg.)
- Metalle und Legierungen
- Ingenieurwesen (insg.)
- Elektrotechnik und Elektronik
- Werkstoffwissenschaften (insg.)
- Werkstoffchemie
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in: Sensors and Actuators, B: Chemical, Jahrgang 113, Nr. 2, 11.08.2005, S. 582-589.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Immunochromatographic assay for determination of botulinum neurotoxin type D
AU - Klewitz, Timo
AU - Gessler, Frank
AU - Beer, Hans
AU - Pflanz, Karl
AU - Scheper, Thomas
PY - 2005/8/11
Y1 - 2005/8/11
N2 - A highly sensitive combination of a membrane dipstick assay and a flatbed scanner for determination of botulinum toxin type D is described. The immunochromatographic assay is based on a sandwich format using two primary antibodies of distinct specificities and one secondary antibody. One of the primary antibodies was conjugated with colloidal gold (detector reagent), the secondary antibody (capture reagent) was immobilized within a defined detection zone (test line) on a diagnostic cellulose nitrate membrane. In combination with an effective sample pre-treatment, the gold conjugated antibody and the second distinct antibody formed a mobile sandwich complex with the toxin. Within the test line the mobile sandwich complex was immobilized and therefore concentrated by the secondary antibody resulting in a distinct red test line. The intensity of colour of the red test line (signal intensity), which correlated directly with the concentration of BoNT/D in the standard or spiked horse faecal sample, was assessed visually and by computer image analysis using a three-determination analysis. Thus, toxin concentrations down to 50 pg/mL were determined within 3.5 h. The mean relative error of the arithmetic mean of the signal intensity amounted to approximately 7%.
AB - A highly sensitive combination of a membrane dipstick assay and a flatbed scanner for determination of botulinum toxin type D is described. The immunochromatographic assay is based on a sandwich format using two primary antibodies of distinct specificities and one secondary antibody. One of the primary antibodies was conjugated with colloidal gold (detector reagent), the secondary antibody (capture reagent) was immobilized within a defined detection zone (test line) on a diagnostic cellulose nitrate membrane. In combination with an effective sample pre-treatment, the gold conjugated antibody and the second distinct antibody formed a mobile sandwich complex with the toxin. Within the test line the mobile sandwich complex was immobilized and therefore concentrated by the secondary antibody resulting in a distinct red test line. The intensity of colour of the red test line (signal intensity), which correlated directly with the concentration of BoNT/D in the standard or spiked horse faecal sample, was assessed visually and by computer image analysis using a three-determination analysis. Thus, toxin concentrations down to 50 pg/mL were determined within 3.5 h. The mean relative error of the arithmetic mean of the signal intensity amounted to approximately 7%.
KW - Botulinum neurotoxin type D
KW - Colloidal gold
KW - Flatbed scanner
KW - Immunoassay
KW - Immunochromatography
KW - Lateral flow test
UR - http://www.scopus.com/inward/record.url?scp=31844449472&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2005.07.007
DO - 10.1016/j.snb.2005.07.007
M3 - Article
AN - SCOPUS:31844449472
VL - 113
SP - 582
EP - 589
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 2
ER -