Genotypic variability for protoplast regeneration in Saintpaulia ionantha (H. Wendl.)

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Autoren

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  • Julius Kühn-Institut (JKI) Bundesforschungsinstitut für Kulturpflanzen
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Details

OriginalspracheEnglisch
Seiten (von - bis)704-707
Seitenumfang4
FachzeitschriftPlant cell reports
Jahrgang14
Ausgabenummer11
PublikationsstatusVeröffentlicht - Aug. 1995
Extern publiziertJa

Abstract

The behaviour of eleven Saintpaulia ionantha (H. Wendl.) genotypes in protoplast culture was compared. Isolation of protoplasts from young shootlets regenerated in vitro on leaf explants, yielded 0.7 to 1.8 × 106 protoplasts per gram fresh weight. In all cultivars and breeding lines tested, cell divisions were observed. The mean division frequencies varied between 1.0 and 5.0% after 14 days, and between 6.4 and 13.8% after 24 days of culture. In ten genotypes callussing and shoot regeneration were achieved. The difference between the genotypes in shoot regeneration rate, between 2 and 68%, was more pronounced. The comparison of four cytokinins indicated hat thidiazuron was most effective for shoot regeneration, but often resulted in poorer shoot quality than benzylaminopurine.

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Genotypic variability for protoplast regeneration in Saintpaulia ionantha (H. Wendl.). / Winkelmann, Traud; Grunewaldt, Jürgen.
in: Plant cell reports, Jahrgang 14, Nr. 11, 08.1995, S. 704-707.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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AU - Grunewaldt, Jürgen

PY - 1995/8

Y1 - 1995/8

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