Details
| Original language | English |
|---|---|
| Pages (from-to) | 704-707 |
| Number of pages | 4 |
| Journal | Plant cell reports |
| Volume | 14 |
| Issue number | 11 |
| Publication status | Published - Aug 1995 |
| Externally published | Yes |
Abstract
The behaviour of eleven Saintpaulia ionantha (H. Wendl.) genotypes in protoplast culture was compared. Isolation of protoplasts from young shootlets regenerated in vitro on leaf explants, yielded 0.7 to 1.8 × 106 protoplasts per gram fresh weight. In all cultivars and breeding lines tested, cell divisions were observed. The mean division frequencies varied between 1.0 and 5.0% after 14 days, and between 6.4 and 13.8% after 24 days of culture. In ten genotypes callussing and shoot regeneration were achieved. The difference between the genotypes in shoot regeneration rate, between 2 and 68%, was more pronounced. The comparison of four cytokinins indicated hat thidiazuron was most effective for shoot regeneration, but often resulted in poorer shoot quality than benzylaminopurine.
Keywords
- Genotypic differences, Protoplast regeneration, Saintpaulia ionantha
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant cell reports, Vol. 14, No. 11, 08.1995, p. 704-707.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Genotypic variability for protoplast regeneration in Saintpaulia ionantha (H. Wendl.)
AU - Winkelmann, Traud
AU - Grunewaldt, Jürgen
PY - 1995/8
Y1 - 1995/8
N2 - The behaviour of eleven Saintpaulia ionantha (H. Wendl.) genotypes in protoplast culture was compared. Isolation of protoplasts from young shootlets regenerated in vitro on leaf explants, yielded 0.7 to 1.8 × 106 protoplasts per gram fresh weight. In all cultivars and breeding lines tested, cell divisions were observed. The mean division frequencies varied between 1.0 and 5.0% after 14 days, and between 6.4 and 13.8% after 24 days of culture. In ten genotypes callussing and shoot regeneration were achieved. The difference between the genotypes in shoot regeneration rate, between 2 and 68%, was more pronounced. The comparison of four cytokinins indicated hat thidiazuron was most effective for shoot regeneration, but often resulted in poorer shoot quality than benzylaminopurine.
AB - The behaviour of eleven Saintpaulia ionantha (H. Wendl.) genotypes in protoplast culture was compared. Isolation of protoplasts from young shootlets regenerated in vitro on leaf explants, yielded 0.7 to 1.8 × 106 protoplasts per gram fresh weight. In all cultivars and breeding lines tested, cell divisions were observed. The mean division frequencies varied between 1.0 and 5.0% after 14 days, and between 6.4 and 13.8% after 24 days of culture. In ten genotypes callussing and shoot regeneration were achieved. The difference between the genotypes in shoot regeneration rate, between 2 and 68%, was more pronounced. The comparison of four cytokinins indicated hat thidiazuron was most effective for shoot regeneration, but often resulted in poorer shoot quality than benzylaminopurine.
KW - Genotypic differences
KW - Protoplast regeneration
KW - Saintpaulia ionantha
UR - http://www.scopus.com/inward/record.url?scp=0028797390&partnerID=8YFLogxK
U2 - 10.1007/BF00232651
DO - 10.1007/BF00232651
M3 - Article
AN - SCOPUS:0028797390
VL - 14
SP - 704
EP - 707
JO - Plant cell reports
JF - Plant cell reports
SN - 0721-7714
IS - 11
ER -