Determination of the DNA/RNA-Associated Subproteome from Chloroplasts and Other Plastid Types

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Maha Chieb
  • Monique Liebers
  • Fabien Chevalier
  • Silva Lerbs-Mache
  • Robert Blanvillain
  • Thomas Pfannschmidt

Externe Organisationen

  • Laboratoire Physiologie Cellulaire & Végétale
  • Centre national de la recherche scientifique (CNRS)
  • Institut national de recherche pour l’agriculture, l’alimentation et l’environnement (INRAE)
  • Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
  • Université Grenoble Alpes (UGA)
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)253-271
Seitenumfang19
FachzeitschriftMethods in molecular biology (Clifton, N.J.)
Jahrgang1829
PublikationsstatusVeröffentlicht - 2018
Extern publiziertJa

Abstract

Plastids of plant and algae cells are of endosymbiotic origin. They possess their own genome and a sophisticated protein machinery to express it. Studies over the recent years uncovered that the regulation of plastid gene expression is highly complex involving a multiplicity of regulatory protein factors that are mostly imported from the cytosol. Proper expression of the chloroplast genome in coordination with nuclear genome was found to be absolutely essential for efficient growth and development of plants especially during early steps of photomorphogenesis, but also at later stages of the plant life cycle. Protein factors being responsible for such essential steps, therefore, are highly interesting for fundamental science as well as for industrial applications targeting crop improvement and yield increase. Nevertheless, many proteins involved in regulation of plastid gene expression are still unidentified and/or uncharacterized. This asks for appropriate methods to analyze this special subproteome. Here, we describe suitable methods that proved to be successful in the analysis of the plastid subproteome of DNA/RNA-binding proteins.

ASJC Scopus Sachgebiete

  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Genetik
  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Molekularbiologie

Zitieren

Determination of the DNA/RNA-Associated Subproteome from Chloroplasts and Other Plastid Types. / Chieb, Maha; Liebers, Monique; Chevalier, Fabien et al.
in: Methods in molecular biology (Clifton, N.J.), Jahrgang 1829, 2018, S. 253-271.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Chieb, M, Liebers, M, Chevalier, F, Lerbs-Mache, S, Blanvillain, R & Pfannschmidt, T 2018, 'Determination of the DNA/RNA-Associated Subproteome from Chloroplasts and Other Plastid Types', Methods in molecular biology (Clifton, N.J.), Jg. 1829, S. 253-271. https://doi.org/10.1007/978-1-4939-8654-5_17
Chieb, M., Liebers, M., Chevalier, F., Lerbs-Mache, S., Blanvillain, R., & Pfannschmidt, T. (2018). Determination of the DNA/RNA-Associated Subproteome from Chloroplasts and Other Plastid Types. Methods in molecular biology (Clifton, N.J.), 1829, 253-271. https://doi.org/10.1007/978-1-4939-8654-5_17
Chieb M, Liebers M, Chevalier F, Lerbs-Mache S, Blanvillain R, Pfannschmidt T. Determination of the DNA/RNA-Associated Subproteome from Chloroplasts and Other Plastid Types. Methods in molecular biology (Clifton, N.J.). 2018;1829:253-271. doi: 10.1007/978-1-4939-8654-5_17
Chieb, Maha ; Liebers, Monique ; Chevalier, Fabien et al. / Determination of the DNA/RNA-Associated Subproteome from Chloroplasts and Other Plastid Types. in: Methods in molecular biology (Clifton, N.J.). 2018 ; Jahrgang 1829. S. 253-271.
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AU - Liebers, Monique

AU - Chevalier, Fabien

AU - Lerbs-Mache, Silva

AU - Blanvillain, Robert

AU - Pfannschmidt, Thomas

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2018.

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Y1 - 2018

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