Details
| Original language | English |
|---|---|
| Pages (from-to) | 253-271 |
| Number of pages | 19 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 1829 |
| Publication status | Published - 2018 |
| Externally published | Yes |
Abstract
Plastids of plant and algae cells are of endosymbiotic origin. They possess their own genome and a sophisticated protein machinery to express it. Studies over the recent years uncovered that the regulation of plastid gene expression is highly complex involving a multiplicity of regulatory protein factors that are mostly imported from the cytosol. Proper expression of the chloroplast genome in coordination with nuclear genome was found to be absolutely essential for efficient growth and development of plants especially during early steps of photomorphogenesis, but also at later stages of the plant life cycle. Protein factors being responsible for such essential steps, therefore, are highly interesting for fundamental science as well as for industrial applications targeting crop improvement and yield increase. Nevertheless, many proteins involved in regulation of plastid gene expression are still unidentified and/or uncharacterized. This asks for appropriate methods to analyze this special subproteome. Here, we describe suitable methods that proved to be successful in the analysis of the plastid subproteome of DNA/RNA-binding proteins.
Keywords
- Chloroplasts/metabolism, DNA-Binding Proteins/metabolism, Electrophoresis, Gel, Two-Dimensional, Mass Spectrometry, Plant Proteins/analysis, Plastids/metabolism, Proteome, Proteomics/methods, RNA-Binding Proteins/metabolism, 2D-SDS-PAGE with isoelectric focussing, Plastid gene expression, 2D-BN-PAGE, DNA-/RNA-binding proteins
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
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In: Methods in molecular biology (Clifton, N.J.), Vol. 1829, 2018, p. 253-271.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Determination of the DNA/RNA-Associated Subproteome from Chloroplasts and Other Plastid Types
AU - Chieb, Maha
AU - Liebers, Monique
AU - Chevalier, Fabien
AU - Lerbs-Mache, Silva
AU - Blanvillain, Robert
AU - Pfannschmidt, Thomas
N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2018.
PY - 2018
Y1 - 2018
N2 - Plastids of plant and algae cells are of endosymbiotic origin. They possess their own genome and a sophisticated protein machinery to express it. Studies over the recent years uncovered that the regulation of plastid gene expression is highly complex involving a multiplicity of regulatory protein factors that are mostly imported from the cytosol. Proper expression of the chloroplast genome in coordination with nuclear genome was found to be absolutely essential for efficient growth and development of plants especially during early steps of photomorphogenesis, but also at later stages of the plant life cycle. Protein factors being responsible for such essential steps, therefore, are highly interesting for fundamental science as well as for industrial applications targeting crop improvement and yield increase. Nevertheless, many proteins involved in regulation of plastid gene expression are still unidentified and/or uncharacterized. This asks for appropriate methods to analyze this special subproteome. Here, we describe suitable methods that proved to be successful in the analysis of the plastid subproteome of DNA/RNA-binding proteins.
AB - Plastids of plant and algae cells are of endosymbiotic origin. They possess their own genome and a sophisticated protein machinery to express it. Studies over the recent years uncovered that the regulation of plastid gene expression is highly complex involving a multiplicity of regulatory protein factors that are mostly imported from the cytosol. Proper expression of the chloroplast genome in coordination with nuclear genome was found to be absolutely essential for efficient growth and development of plants especially during early steps of photomorphogenesis, but also at later stages of the plant life cycle. Protein factors being responsible for such essential steps, therefore, are highly interesting for fundamental science as well as for industrial applications targeting crop improvement and yield increase. Nevertheless, many proteins involved in regulation of plastid gene expression are still unidentified and/or uncharacterized. This asks for appropriate methods to analyze this special subproteome. Here, we describe suitable methods that proved to be successful in the analysis of the plastid subproteome of DNA/RNA-binding proteins.
KW - Chloroplasts/metabolism
KW - DNA-Binding Proteins/metabolism
KW - Electrophoresis, Gel, Two-Dimensional
KW - Mass Spectrometry
KW - Plant Proteins/analysis
KW - Plastids/metabolism
KW - Proteome
KW - Proteomics/methods
KW - RNA-Binding Proteins/metabolism
KW - 2D-SDS-PAGE with isoelectric focussing
KW - Plastid gene expression
KW - 2D-BN-PAGE
KW - DNA-/RNA-binding proteins
UR - http://www.scopus.com/inward/record.url?scp=85050011945&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-8654-5_17
DO - 10.1007/978-1-4939-8654-5_17
M3 - Article
C2 - 29987727
VL - 1829
SP - 253
EP - 271
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -