Details
| Original language | English |
|---|---|
| Article number | 1190962 |
| Journal | Frontiers in microbiology |
| Volume | 14 |
| Publication status | Published - 18 Jul 2023 |
Abstract
Uranium (U) contamination of the environment causes high risk to health, demanding for effective and sustainable remediation. Bioremediation via microbial reduction of soluble U(VI) is generating high fractions (>50%) of insoluble non-crystalline U(IV) which, however, might be remobilized by sulfur-oxidizing bacteria. In this study, the efficacy of Acidithiobacillus (At.) ferrooxidans and Thiobacillus (T.) denitrificans to mobilize non-crystalline U(IV) and associated U isotope fractionation were investigated. At. ferrooxidans mobilized between 74 and 91% U after 1 week, and U mobilization was observed for both, living and inactive cells. Contrary to previous observations, no mobilization by T. denitrificans could be observed. Uranium mobilization by At. ferrooxidans did not cause U isotope fractionation suggesting that U isotope ratio determination is unsuitable as a direct proxy for bacterial U remobilization. The similar mobilization capability of active and inactive At. ferrooxidans cells suggests that the mobilization is based on the reaction with the cell biomass. This study raises doubts about the long-term sustainability of in-situ bioremediation measures at U-contaminated sites, especially with regard to non-crystalline U(IV) being the main component of U bioremediation.
Keywords
- Acidithiobacillus ferrooxidans, isotope fractionation, laboratory batch experiments, remobilization, Thiobacillus denitrificans, uranium
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Medicine(all)
- Microbiology (medical)
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In: Frontiers in microbiology, Vol. 14, 1190962, 18.07.2023.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - U mobilization and associated U isotope fractionation by sulfur-oxidizing bacteria
AU - Rosendahl, C. D.
AU - Roebbert, Y.
AU - Schippers, A.
AU - Weyer, S.
N1 - Funding Information: This work was funded by the DFG/SNSF grants (WE 2850-16/1 and 200021E-164209: Fate of tetravalent uranium under reducing conditions).
PY - 2023/7/18
Y1 - 2023/7/18
N2 - Uranium (U) contamination of the environment causes high risk to health, demanding for effective and sustainable remediation. Bioremediation via microbial reduction of soluble U(VI) is generating high fractions (>50%) of insoluble non-crystalline U(IV) which, however, might be remobilized by sulfur-oxidizing bacteria. In this study, the efficacy of Acidithiobacillus (At.) ferrooxidans and Thiobacillus (T.) denitrificans to mobilize non-crystalline U(IV) and associated U isotope fractionation were investigated. At. ferrooxidans mobilized between 74 and 91% U after 1 week, and U mobilization was observed for both, living and inactive cells. Contrary to previous observations, no mobilization by T. denitrificans could be observed. Uranium mobilization by At. ferrooxidans did not cause U isotope fractionation suggesting that U isotope ratio determination is unsuitable as a direct proxy for bacterial U remobilization. The similar mobilization capability of active and inactive At. ferrooxidans cells suggests that the mobilization is based on the reaction with the cell biomass. This study raises doubts about the long-term sustainability of in-situ bioremediation measures at U-contaminated sites, especially with regard to non-crystalline U(IV) being the main component of U bioremediation.
AB - Uranium (U) contamination of the environment causes high risk to health, demanding for effective and sustainable remediation. Bioremediation via microbial reduction of soluble U(VI) is generating high fractions (>50%) of insoluble non-crystalline U(IV) which, however, might be remobilized by sulfur-oxidizing bacteria. In this study, the efficacy of Acidithiobacillus (At.) ferrooxidans and Thiobacillus (T.) denitrificans to mobilize non-crystalline U(IV) and associated U isotope fractionation were investigated. At. ferrooxidans mobilized between 74 and 91% U after 1 week, and U mobilization was observed for both, living and inactive cells. Contrary to previous observations, no mobilization by T. denitrificans could be observed. Uranium mobilization by At. ferrooxidans did not cause U isotope fractionation suggesting that U isotope ratio determination is unsuitable as a direct proxy for bacterial U remobilization. The similar mobilization capability of active and inactive At. ferrooxidans cells suggests that the mobilization is based on the reaction with the cell biomass. This study raises doubts about the long-term sustainability of in-situ bioremediation measures at U-contaminated sites, especially with regard to non-crystalline U(IV) being the main component of U bioremediation.
KW - Acidithiobacillus ferrooxidans
KW - isotope fractionation
KW - laboratory batch experiments
KW - remobilization
KW - Thiobacillus denitrificans
KW - uranium
UR - http://www.scopus.com/inward/record.url?scp=85166437877&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2023.1190962
DO - 10.3389/fmicb.2023.1190962
M3 - Article
AN - SCOPUS:85166437877
VL - 14
JO - Frontiers in microbiology
JF - Frontiers in microbiology
SN - 1664-302X
M1 - 1190962
ER -