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Two FIA-based biosensor systems studied for bioprocess monitoring

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Authors

  • Th Scheper
  • W. Brandes
  • H. Maschke
  • F. Plötz

External Research Organisations

  • University of Münster
  • Genentech Incorporated
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    • Citation Indexes: 29
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Details

Original languageEnglish
Pages (from-to)345-356
Number of pages12
JournalJournal of biotechnology
Volume31
Issue number3
Publication statusPublished - Dec 1993
Externally publishedYes

Abstract

In this paper, two different FIA-based biosensor systems are described for application to different biotechnologically relevant purposes. In the first system, single fiber optodes were used to determine the pH, urea and penicillin V concentrations. A two-channel system was developed for the simultaneous monitoring of different variables to increase the analysis accuracy. This system was used for monitoring the penicillin V concentration during a cultivation of Penicillium chrysogenum. The second system described is a calorimetric immunoassay based on the use of an enzyme thermistor. A sandwich assay with protein A immobilized on a solid support for the determination of various IgGs was established. A fusion protein of protein A and β-galactosidase obtained from a recombinant E. coli strain was used in the labelling and detection reaction. This system is designed for future application in bioprocess monitoring.

Keywords

    Biosensor, Calorimetry, FIA, Flow injection analysis, Immunoanalysis, Optode

ASJC Scopus subject areas

Cite this

Two FIA-based biosensor systems studied for bioprocess monitoring. / Scheper, Th; Brandes, W.; Maschke, H. et al.
In: Journal of biotechnology, Vol. 31, No. 3, 12.1993, p. 345-356.

Research output: Contribution to journalArticleResearchpeer review

Scheper T, Brandes W, Maschke H, Plötz F, Müller C. Two FIA-based biosensor systems studied for bioprocess monitoring. Journal of biotechnology. 1993 Dec;31(3):345-356. doi: 10.1016/0168-1656(93)90079-3
Scheper, Th ; Brandes, W. ; Maschke, H. et al. / Two FIA-based biosensor systems studied for bioprocess monitoring. In: Journal of biotechnology. 1993 ; Vol. 31, No. 3. pp. 345-356.
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AU - Scheper, Th

AU - Brandes, W.

AU - Maschke, H.

AU - Plötz, F.

AU - Müller, C.

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KW - Flow injection analysis

KW - Immunoanalysis

KW - Optode

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