Details
| Original language | English |
|---|---|
| Pages (from-to) | 83-89 |
| Number of pages | 7 |
| Journal | Physiological and Molecular Plant Pathology |
| Volume | 75 |
| Issue number | 3 |
| Publication status | Published - Jan 2011 |
Abstract
Silicon induced resistance (SiIR) in tomato against bacterial wilt caused by Ralstonia solanacearum. The mechanism of SiIR is ambiguous. Therefore we tracked the expression of twelve defense marker genes over time using qRT-PCR. Besides up-regulated expression of the jasmonic acid/ethylene marker genes JERF3, TSRF1and ACCO during SiIR, the expression of the oxidative stress markers FD-I and POD and the basal defense marker AGP-1g were also up-regulated. The expression of defense marker genes was generally induced upon challenging the silicon-treated plants with R. solanacearum and reached its highest levels at 72 h post inoculation. A global transcriptome profiling was performed to uncover further changes at this time point using the TOM2 microarray. Significant regulation of additional 16 genes was revealed after silicon treatment in plants challenged with R. solanacearum. Twelve genes, involved in defense, signal transduction, response to stresses, transcription, ubiquitinylation and metabolism, were up-regulated. A JAZ1-similar gene showed the highest level of up-regulation indicating prior induction and fine tuning of jasmonic acid signaling. Thus, we conclude that silicon primed plants, thereby alleviating biotic stress imposed by the pathogen. The primed state could be mediated via ethylene, jasmonic acid and/or reactive oxygen species signaling pathways. We also suggest that the protective role of silicon can not only be explained with its mechanical properties.
Keywords
- Ethylene, Gene expression, Induced resistance, Jasmonic acid, Priming, Ralstonia solanacearum, Silicon, Solanum lycopersicum
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Physiological and Molecular Plant Pathology, Vol. 75, No. 3, 01.2011, p. 83-89.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Transcriptome of silicon-induced resistance against Ralstonia solanacearum in the silicon non-accumulator tomato implicates priming effect
AU - Ghareeb, Hassan
AU - Bozsó, Zoltán
AU - Ott, Peter G.
AU - Repenning, Cornelia
AU - Stahl, Frank
AU - Wydra, Kerstin
N1 - Funding Information: This research was supported by an award to K. Wydra for ‘Projects in agricultural sciences to be specifically awarded’ of the ‘Stifterverband für die Deutsche Wissenschaft’, and for Z. Bozsó and P.G. Ott by grants of the Hungarian National Science Foundation , AT-049318 and K68386 . Copyright: Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/1
Y1 - 2011/1
N2 - Silicon induced resistance (SiIR) in tomato against bacterial wilt caused by Ralstonia solanacearum. The mechanism of SiIR is ambiguous. Therefore we tracked the expression of twelve defense marker genes over time using qRT-PCR. Besides up-regulated expression of the jasmonic acid/ethylene marker genes JERF3, TSRF1and ACCO during SiIR, the expression of the oxidative stress markers FD-I and POD and the basal defense marker AGP-1g were also up-regulated. The expression of defense marker genes was generally induced upon challenging the silicon-treated plants with R. solanacearum and reached its highest levels at 72 h post inoculation. A global transcriptome profiling was performed to uncover further changes at this time point using the TOM2 microarray. Significant regulation of additional 16 genes was revealed after silicon treatment in plants challenged with R. solanacearum. Twelve genes, involved in defense, signal transduction, response to stresses, transcription, ubiquitinylation and metabolism, were up-regulated. A JAZ1-similar gene showed the highest level of up-regulation indicating prior induction and fine tuning of jasmonic acid signaling. Thus, we conclude that silicon primed plants, thereby alleviating biotic stress imposed by the pathogen. The primed state could be mediated via ethylene, jasmonic acid and/or reactive oxygen species signaling pathways. We also suggest that the protective role of silicon can not only be explained with its mechanical properties.
AB - Silicon induced resistance (SiIR) in tomato against bacterial wilt caused by Ralstonia solanacearum. The mechanism of SiIR is ambiguous. Therefore we tracked the expression of twelve defense marker genes over time using qRT-PCR. Besides up-regulated expression of the jasmonic acid/ethylene marker genes JERF3, TSRF1and ACCO during SiIR, the expression of the oxidative stress markers FD-I and POD and the basal defense marker AGP-1g were also up-regulated. The expression of defense marker genes was generally induced upon challenging the silicon-treated plants with R. solanacearum and reached its highest levels at 72 h post inoculation. A global transcriptome profiling was performed to uncover further changes at this time point using the TOM2 microarray. Significant regulation of additional 16 genes was revealed after silicon treatment in plants challenged with R. solanacearum. Twelve genes, involved in defense, signal transduction, response to stresses, transcription, ubiquitinylation and metabolism, were up-regulated. A JAZ1-similar gene showed the highest level of up-regulation indicating prior induction and fine tuning of jasmonic acid signaling. Thus, we conclude that silicon primed plants, thereby alleviating biotic stress imposed by the pathogen. The primed state could be mediated via ethylene, jasmonic acid and/or reactive oxygen species signaling pathways. We also suggest that the protective role of silicon can not only be explained with its mechanical properties.
KW - Ethylene
KW - Gene expression
KW - Induced resistance
KW - Jasmonic acid
KW - Priming
KW - Ralstonia solanacearum
KW - Silicon
KW - Solanum lycopersicum
UR - http://www.scopus.com/inward/record.url?scp=79151473064&partnerID=8YFLogxK
U2 - 10.1016/j.pmpp.2010.11.004
DO - 10.1016/j.pmpp.2010.11.004
M3 - Article
AN - SCOPUS:79151473064
VL - 75
SP - 83
EP - 89
JO - Physiological and Molecular Plant Pathology
JF - Physiological and Molecular Plant Pathology
SN - 0885-5765
IS - 3
ER -