Details
| Original language | English |
|---|---|
| Pages (from-to) | 89-95 |
| Number of pages | 7 |
| Journal | Plant science |
| Volume | 124 |
| Issue number | 1 |
| Publication status | Published - 18 Apr 1997 |
| Externally published | Yes |
Abstract
A full-length transcript sequence encoding the broad bean (Vicia faba L.) asparagine synthetase (AS) VfAS1 was isolated from a nodule cDNA library. Sequence homologies indicated that VfAS1 belonged to the glutamine-dependent type of AS enzymes. The corresponding gene was highly expressed in root nodules and at a three-fold lower level in uninfected roots. Additionally, traces of VfAS1 transcripts were detected in epicotyl and stem tissues. Tissue-print hybridizations revealed that the VfAS1 gene was strongly expressed in the nitrogen-fixing zone III of root nodules, VfAS1 transcripts were absent from the meristem, the prefixing zone II as well as from peripheral nodule tissues.
Keywords
- Nodule nitrogen metabolism, Nodule-specific cDNA library, Tissue-print hybridization
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant science, Vol. 124, No. 1, 18.04.1997, p. 89-95.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The asparagine synthetase gene VfAS1 is strongly expressed in the nitrogen-fixing zone of broad bean (Vicia faba L.) root nodules
AU - Küster, Helge
AU - Albus, Ulrike
AU - Frühling, Martin
AU - Tchetkova, Svetlana A.
AU - Tikhonovitch, Igor A.
AU - Pühler, Alfred
AU - Perlick, Andreas M.
N1 - Funding information: S.A. Tchetkova and I.A. Tikhonovitch acknowledge grants from the ‘Bundesministerium fur Ernahrung, Landwirtschaft und Forsten’ (BML).
PY - 1997/4/18
Y1 - 1997/4/18
N2 - A full-length transcript sequence encoding the broad bean (Vicia faba L.) asparagine synthetase (AS) VfAS1 was isolated from a nodule cDNA library. Sequence homologies indicated that VfAS1 belonged to the glutamine-dependent type of AS enzymes. The corresponding gene was highly expressed in root nodules and at a three-fold lower level in uninfected roots. Additionally, traces of VfAS1 transcripts were detected in epicotyl and stem tissues. Tissue-print hybridizations revealed that the VfAS1 gene was strongly expressed in the nitrogen-fixing zone III of root nodules, VfAS1 transcripts were absent from the meristem, the prefixing zone II as well as from peripheral nodule tissues.
AB - A full-length transcript sequence encoding the broad bean (Vicia faba L.) asparagine synthetase (AS) VfAS1 was isolated from a nodule cDNA library. Sequence homologies indicated that VfAS1 belonged to the glutamine-dependent type of AS enzymes. The corresponding gene was highly expressed in root nodules and at a three-fold lower level in uninfected roots. Additionally, traces of VfAS1 transcripts were detected in epicotyl and stem tissues. Tissue-print hybridizations revealed that the VfAS1 gene was strongly expressed in the nitrogen-fixing zone III of root nodules, VfAS1 transcripts were absent from the meristem, the prefixing zone II as well as from peripheral nodule tissues.
KW - Nodule nitrogen metabolism
KW - Nodule-specific cDNA library
KW - Tissue-print hybridization
UR - http://www.scopus.com/inward/record.url?scp=0343416950&partnerID=8YFLogxK
U2 - 10.1016/S0168-9452(97)04607-4
DO - 10.1016/S0168-9452(97)04607-4
M3 - Article
AN - SCOPUS:0343416950
VL - 124
SP - 89
EP - 95
JO - Plant science
JF - Plant science
SN - 0168-9452
IS - 1
ER -