Details
| Original language | English |
|---|---|
| Pages (from-to) | 47-53 |
| Number of pages | 7 |
| Journal | Current Opinion in Microbiology |
| Volume | 14 |
| Issue number | 1 |
| Publication status | Published - Feb 2011 |
| Externally published | Yes |
Abstract
TAL (transcription activator- like) effectors constitute a novel class of DNA-binding proteins with predictable specificity. They are employed by Gram-negative plant-pathogenic bacteria of the genus Xanthomonas which translocate a cocktail of different effector proteins via a type III secretion system (T3SS) into plant cells where they serve as virulence determinants. Inside the plant cell, TALs localize to the nucleus, bind to target promoters, and induce expression of plant genes. DNA-binding specificity of TALs is determined by a central domain of tandem repeats. Each repeat confers recognition of one base pair (bp) in the DNA. Rearrangement of repeat modules allows design of proteins with desired DNA-binding specificities. Here, we summarize how TAL specificity is encoded, first structural data and first data on site-specific TAL nucleases.
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Medicine(all)
- Microbiology (medical)
- Medicine(all)
- Infectious Diseases
Sustainable Development Goals
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In: Current Opinion in Microbiology, Vol. 14, No. 1, 02.2011, p. 47-53.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - TAL effectors are remote controls for gene activation
AU - Scholze, Heidi
AU - Boch, Jens
N1 - Funding Information: We thank D. Büttner, R. Koebnik, and U. Bonas for discussions and helpful suggestions on the manuscript. Research in our laboratory is supported by a grant from the Deutsche Forschungsgemeinschaft (SPP 1212) to J.B. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/2
Y1 - 2011/2
N2 - TAL (transcription activator- like) effectors constitute a novel class of DNA-binding proteins with predictable specificity. They are employed by Gram-negative plant-pathogenic bacteria of the genus Xanthomonas which translocate a cocktail of different effector proteins via a type III secretion system (T3SS) into plant cells where they serve as virulence determinants. Inside the plant cell, TALs localize to the nucleus, bind to target promoters, and induce expression of plant genes. DNA-binding specificity of TALs is determined by a central domain of tandem repeats. Each repeat confers recognition of one base pair (bp) in the DNA. Rearrangement of repeat modules allows design of proteins with desired DNA-binding specificities. Here, we summarize how TAL specificity is encoded, first structural data and first data on site-specific TAL nucleases.
AB - TAL (transcription activator- like) effectors constitute a novel class of DNA-binding proteins with predictable specificity. They are employed by Gram-negative plant-pathogenic bacteria of the genus Xanthomonas which translocate a cocktail of different effector proteins via a type III secretion system (T3SS) into plant cells where they serve as virulence determinants. Inside the plant cell, TALs localize to the nucleus, bind to target promoters, and induce expression of plant genes. DNA-binding specificity of TALs is determined by a central domain of tandem repeats. Each repeat confers recognition of one base pair (bp) in the DNA. Rearrangement of repeat modules allows design of proteins with desired DNA-binding specificities. Here, we summarize how TAL specificity is encoded, first structural data and first data on site-specific TAL nucleases.
UR - http://www.scopus.com/inward/record.url?scp=79551687772&partnerID=8YFLogxK
U2 - 10.1016/j.mib.2010.12.001
DO - 10.1016/j.mib.2010.12.001
M3 - Review article
C2 - 21215685
AN - SCOPUS:79551687772
VL - 14
SP - 47
EP - 53
JO - Current Opinion in Microbiology
JF - Current Opinion in Microbiology
SN - 1369-5274
IS - 1
ER -