Synonymous rpsH variants: the common denominator in Escherichia coli adapting to ionizing radiation

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Original languageEnglish
Article numberlqae110
JournalNAR Genomics and Bioinformatics
Volume6
Issue number3
Early online date24 Aug 2024
Publication statusPublished - Sept 2024

Abstract

Ionizing radiation (IR) in high doses is generally lethal to most organisms. Investigating mechanisms of radiation resistance is crucial for gaining insights into the underlying cellular responses and understanding the damaging effects of IR. In this study, we conducted a comprehensive analysis of sequencing data from an evolutionary experiment aimed at understanding the genetic adaptations to ionizing radiation in Escherichia coli. By including previously neglected synonymous mutations, we identified the rpsH c.294T > G variant, which emerged in all 17 examined isolates across four subpopulations. The identified variant is a synonymous mutation affecting the 30S ribosomal protein S8, and consistently exhibited high detection and low allele frequencies in all subpopulations. This variant, along with two additional rpsH variants, potentially influences translational control of the ribosomal spc operon. The early emergence and stability of these variants suggest their role in adapting to environmental stress, possibly contributing to radiation resistance. Our findings shed light on the dynamics of ribosomal variants during the evolutionary process and their potential role in stress adaptation, providing valuable implications for understanding clinical radiation sensitivity and improving radiotherapy.

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Synonymous rpsH variants: the common denominator in Escherichia coli adapting to ionizing radiation. / Stemwedel, Katharina; Haase, Nadin; Christ, Simon et al.
In: NAR Genomics and Bioinformatics, Vol. 6, No. 3, lqae110, 09.2024.

Research output: Contribution to journalArticleResearchpeer review

Stemwedel K, Haase N, Christ S, Bogdanova NV, Rudorf S. Synonymous rpsH variants: the common denominator in Escherichia coli adapting to ionizing radiation. NAR Genomics and Bioinformatics. 2024 Sept;6(3):lqae110. Epub 2024 Aug 24. doi: 10.1093/nargab/lqae110
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Download

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T2 - the common denominator in Escherichia coli adapting to ionizing radiation

AU - Stemwedel, Katharina

AU - Haase, Nadin

AU - Christ, Simon

AU - Bogdanova, Natalia V

AU - Rudorf, Sophia

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