Somatic versus zygotic embryogenesis: Learning from seeds

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Original languageEnglish
Number of pages22
ISBN (electronic)978-1-4939-3061-6
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1359
ISSN (Print)1064-3745

Abstract

Plant embryogenesis is a fascinating developmental program that is very successfully established in nature in seeds. In case of in vitro somatic embryogenesis this process is subjected to several limitations such as asynchronous differentiation and further development of somatic embryos, malformations and disturbed polarity, precocious germination, lack of maturity, early loss of embryogenic potential, and strong genotypic differences in the regeneration efficiency. Several studies have shown the similarity of somatic and zygotic embryos in terms of morphological, histological, biochemical, and physiological aspects. However, pronounced differences have also been reported and refer to much higher stress levels, less accumulation of storage compounds and a missing distinction of differentiation and germination by a quiescent phase in somatic embryos. Here, an overview on recent literature describing both embryogenesis pathways, comparing somatic and zygotic embryos and analyzing the role of the endosperm is presented. By taking zygotic embryos as the reference and learning from the situation in seeds, somatic embryogenesis can be improved and optimized in order to make use of the enormous potential this regeneration pathway offers for plant propagation and breeding.

Keywords

    Biochemistry, Comparative approach, Maturation, Morphology, Proteome, Storage reserves, Stress response, Transcriptome

ASJC Scopus subject areas

Cite this

Somatic versus zygotic embryogenesis: Learning from seeds. / Winkelmann, T.
2016. 22 p. (Methods in Molecular Biology; Vol. 1359).

Research output: Book/ReportMonographResearch

Winkelmann T. Somatic versus zygotic embryogenesis: Learning from seeds. 2016. 22 p. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-3061-6_2
Winkelmann, T. / Somatic versus zygotic embryogenesis: Learning from seeds. 2016. 22 p. (Methods in Molecular Biology).
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