Details
| Original language | English |
|---|---|
| Title of host publication | RNA Spectroscopy |
| Subtitle of host publication | Methods and Protocols |
| Place of Publication | New York |
| Pages | 165-188 |
| Number of pages | 24 |
| ISBN (electronic) | 9781071602782 |
| Publication status | Published - 1 Feb 2020 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2113 |
| ISSN (Print) | 1064-3745 |
| ISSN (electronic) | 1940-6029 |
Abstract
Small-angle neutron scattering (SANS) provides structural information on biomacromolecules and their complexes in dilute solutions at the nanometer length scale. The overall dimensions, shapes, and interactions can be probed and compared to information obtained by complementary structural biology techniques such as crystallography, NMR, and EM. SANS, in combination with solvent H2O/D2O exchange and/or deuteration, is particularly well suited to probe the internal structure of RNA–protein (RNP) complexes since neutrons are more sensitive than X-rays to the difference in scattering length densities of proteins and RNA, with respect to an aqueous solvent. In this book chapter we provide a practical guide on how to carry out SANS experiments on RNP complexes, as well as possibilities of data analysis and interpretation.
Keywords
- Contrast variation, Deuterium labeling, Macromolecular complex, Protein, RNA, RNP, Small-angle neutron scattering (SANS), Structural biology
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
Cite this
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RNA Spectroscopy: Methods and Protocols. New York, 2020. p. 165-188 (Methods in Molecular Biology; Vol. 2113).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Small-Angle Neutron Scattering of RNA–Protein Complexes
AU - Lapinaite, Audrone
AU - Carlomagno, Teresa
AU - Gabel, Frank
N1 - Funding Information: FG acknowledges funding from the French Agence Nationale de la Recherche (grant ANR-15-CE11-0026-01 ?PROTstretch?). TC acknowledges funding from the DFG (grants CA294/3-2 and CA294/11-1).
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Small-angle neutron scattering (SANS) provides structural information on biomacromolecules and their complexes in dilute solutions at the nanometer length scale. The overall dimensions, shapes, and interactions can be probed and compared to information obtained by complementary structural biology techniques such as crystallography, NMR, and EM. SANS, in combination with solvent H2O/D2O exchange and/or deuteration, is particularly well suited to probe the internal structure of RNA–protein (RNP) complexes since neutrons are more sensitive than X-rays to the difference in scattering length densities of proteins and RNA, with respect to an aqueous solvent. In this book chapter we provide a practical guide on how to carry out SANS experiments on RNP complexes, as well as possibilities of data analysis and interpretation.
AB - Small-angle neutron scattering (SANS) provides structural information on biomacromolecules and their complexes in dilute solutions at the nanometer length scale. The overall dimensions, shapes, and interactions can be probed and compared to information obtained by complementary structural biology techniques such as crystallography, NMR, and EM. SANS, in combination with solvent H2O/D2O exchange and/or deuteration, is particularly well suited to probe the internal structure of RNA–protein (RNP) complexes since neutrons are more sensitive than X-rays to the difference in scattering length densities of proteins and RNA, with respect to an aqueous solvent. In this book chapter we provide a practical guide on how to carry out SANS experiments on RNP complexes, as well as possibilities of data analysis and interpretation.
KW - Contrast variation
KW - Deuterium labeling
KW - Macromolecular complex
KW - Protein
KW - RNA
KW - RNP
KW - Small-angle neutron scattering (SANS)
KW - Structural biology
UR - http://www.scopus.com/inward/record.url?scp=85078854842&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-0278-2_13
DO - 10.1007/978-1-0716-0278-2_13
M3 - Contribution to book/anthology
C2 - 32006315
AN - SCOPUS:85078854842
SN - 9781071602775
T3 - Methods in Molecular Biology
SP - 165
EP - 188
BT - RNA Spectroscopy
CY - New York
ER -