Row-like organization of ATP synthase in intact mitochondria determined by cryo-electron tomography

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Natalya V. Dudkina
  • Gert T. Oostergetel
  • Dagmar Lewejohann
  • Hans Peter Braun
  • Egbert J. Boekema

External Research Organisations

  • University of Groningen
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Details

Original languageEnglish
Pages (from-to)272-277
Number of pages6
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1797
Issue number2
Publication statusPublished - 17 Nov 2009

Abstract

The fine structure of intact, close-to-spherical mitochondria from the alga Polytomella was visualized by dual-axis cryo-electron tomography. The supramolecular organization of dimeric ATP synthase in the cristae membranes was investigated by averaging subvolumes of tomograms and 3D details at ∼ 6 nm resolution were revealed. Oligomeric ATP synthase is composed of rows of dimers at 12 nm intervals; the dimers make a slight angle along the row. In addition, the main features of monomeric ATP synthase, such as the conically shaped F1 headpiece, central stalk and stator were revealed. This demonstrates the capability of dual-axis electron tomography to unravel details of proteins and their interactions in complete organelles.

Keywords

    ATP synthase, Electron tomography, Mitochondria, Polytomella

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biophysics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Row-like organization of ATP synthase in intact mitochondria determined by cryo-electron tomography. / Dudkina, Natalya V.; Oostergetel, Gert T.; Lewejohann, Dagmar et al.
In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1797, No. 2, 17.11.2009, p. 272-277.

Research output: Contribution to journalArticleResearchpeer review

Dudkina NV, Oostergetel GT, Lewejohann D, Braun HP, Boekema EJ. Row-like organization of ATP synthase in intact mitochondria determined by cryo-electron tomography. Biochimica et Biophysica Acta - Bioenergetics. 2009 Nov 17;1797(2):272-277. doi: 10.1016/j.bbabio.2009.11.004
Dudkina, Natalya V. ; Oostergetel, Gert T. ; Lewejohann, Dagmar et al. / Row-like organization of ATP synthase in intact mitochondria determined by cryo-electron tomography. In: Biochimica et Biophysica Acta - Bioenergetics. 2009 ; Vol. 1797, No. 2. pp. 272-277.
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AU - Dudkina, Natalya V.

AU - Oostergetel, Gert T.

AU - Lewejohann, Dagmar

AU - Braun, Hans Peter

AU - Boekema, Egbert J.

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