Details
| Original language | English |
|---|---|
| Pages (from-to) | 46-56 |
| Number of pages | 11 |
| Journal | Journal of Structural Biology |
| Volume | 199 |
| Issue number | 1 |
| Publication status | Published - Jul 2017 |
Abstract
Gastroliths are highly calcified structures formed in the cardiac stomach wall of crustaceans for the temporary storage of amorphous CaCO 3 (ACC). The gastrolithic ACC is stabilized by the presence of biomolecules, and represents a novel model for research into biomineralization. For the first time, an in vitro biomimetic retrosynthesis of scaffolds of gastrolithic matrices with CaCO 3 is presented. With the help of synthetic polyacrylic (PAA) and phytic (PA) acids, amorphous precursor particles were stabilized in double (DD) and gas (GD) diffusion crystallization assays. The presence of these synthetic molecules as efficient inhibitors of nucleation and growth of CaCO 3, and the use of biological gastrolith scaffolds as confined reaction environments determined the kinetics of crystallization, and controlled the morphogenesis of CaCO 3. The formation of ACC particles was demonstrated and their crystallization was followed by light microscopy, scanning and transmission electron microscopy, and electron diffraction.
Keywords
- Amorphous calcium carbonate, Double and gas diffusion crystallization, Gastrolith, Polymorphism, Retrosynthesis
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Structural Biology
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In: Journal of Structural Biology, Vol. 199, No. 1, 07.2017, p. 46-56.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Retrosynthesis of CaCO3 via amorphous precursor particles using gastroliths of the Red Claw lobster (Cherax quadricarinatus).
AU - Neira-Carrillo, A
AU - Fernández, MS
AU - Hevia, GP
AU - Arias, JL
AU - Gebauer, D
AU - Cölfen, H
N1 - Funding information: This research was supported by FONDECYT N° 1140660 & 11070136 granted by the Chilean Council for Science and Technology (CONICYT). Dr. Neira-Carrillo would like to thank Prof. Markus Antonietti (MPI of Colloids and Interfaces, Golm, Potsdam, Germany) for providing equipment facilities. DG is a Research Fellow of the Zukunftskolleg of the University of Konstanz.
PY - 2017/7
Y1 - 2017/7
N2 - Gastroliths are highly calcified structures formed in the cardiac stomach wall of crustaceans for the temporary storage of amorphous CaCO 3 (ACC). The gastrolithic ACC is stabilized by the presence of biomolecules, and represents a novel model for research into biomineralization. For the first time, an in vitro biomimetic retrosynthesis of scaffolds of gastrolithic matrices with CaCO 3 is presented. With the help of synthetic polyacrylic (PAA) and phytic (PA) acids, amorphous precursor particles were stabilized in double (DD) and gas (GD) diffusion crystallization assays. The presence of these synthetic molecules as efficient inhibitors of nucleation and growth of CaCO 3, and the use of biological gastrolith scaffolds as confined reaction environments determined the kinetics of crystallization, and controlled the morphogenesis of CaCO 3. The formation of ACC particles was demonstrated and their crystallization was followed by light microscopy, scanning and transmission electron microscopy, and electron diffraction.
AB - Gastroliths are highly calcified structures formed in the cardiac stomach wall of crustaceans for the temporary storage of amorphous CaCO 3 (ACC). The gastrolithic ACC is stabilized by the presence of biomolecules, and represents a novel model for research into biomineralization. For the first time, an in vitro biomimetic retrosynthesis of scaffolds of gastrolithic matrices with CaCO 3 is presented. With the help of synthetic polyacrylic (PAA) and phytic (PA) acids, amorphous precursor particles were stabilized in double (DD) and gas (GD) diffusion crystallization assays. The presence of these synthetic molecules as efficient inhibitors of nucleation and growth of CaCO 3, and the use of biological gastrolith scaffolds as confined reaction environments determined the kinetics of crystallization, and controlled the morphogenesis of CaCO 3. The formation of ACC particles was demonstrated and their crystallization was followed by light microscopy, scanning and transmission electron microscopy, and electron diffraction.
KW - Amorphous calcium carbonate
KW - Double and gas diffusion crystallization
KW - Gastrolith
KW - Polymorphism
KW - Retrosynthesis
UR - http://www.scopus.com/inward/record.url?scp=85019370958&partnerID=8YFLogxK
U2 - 10.1016/j.jsb.2017.05.004
DO - 10.1016/j.jsb.2017.05.004
M3 - Article
C2 - 28506935
VL - 199
SP - 46
EP - 56
JO - Journal of Structural Biology
JF - Journal of Structural Biology
SN - 1047-8477
IS - 1
ER -