Details
| Original language | English |
|---|---|
| Title of host publication | Cell-Based Microarrays |
| Publisher | Springer Verlag |
| Pages | 131-145 |
| Number of pages | 15 |
| ISBN (electronic) | 978-1-4939-7792-5 |
| ISBN (print) | 978-1-4939-7791-8 |
| Publication status | Published - 10 Apr 2018 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1771 |
| ISSN (Print) | 1064-3745 |
Abstract
The deposition of living cells on microarray surfaces can be used to create physiologically relevant architecture in vitro. Such living cell microarrays enable the reconstruction of biological processes outside the body in a miniaturized format and have many advantages over traditional cell culture. The present protocol offers an option for the preparation and analysis of living primary and stem cell-based microarrays utilizing the standard microarray equipment (contact-free piezoelectric nanoprinter, microarray scanner), as well as microscopy. To produce living cell microarrays, we applied two kinds of mesenchymal stem cells (MSCs) isolated from umbilical cord and adipose tissue, as well as human umbilical vein endothelial cells (HUVECs) as model cells. We used live imaging microscopy for the online monitoring of cell spots in total size, staining of viable cells with Calcein acetoxymethyl ester (Calcein-AM) and treatment of MSCs with differentiation media to analyze the proliferation, viability, and differentiation potential of printed cells. This way, the general applicability of the established living cell-based microarray production was demonstrated.
Keywords
- Endothelial cells, Living cell microarrays, Mesenchymal stem cells, Microarray technology, Online monitoring microscopy, Piezoelectric nanoprinting, Primary human cells
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
Cite this
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Cell-Based Microarrays. Springer Verlag, 2018. p. 131-145 (Methods in Molecular Biology; Vol. 1771).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Primary and Stem Cell Microarrays:
T2 - Application as Miniaturized Biotesting Systems
AU - Jonczyk, Rebecca
AU - Scheper, Thomas
AU - Stahl, Frank
PY - 2018/4/10
Y1 - 2018/4/10
N2 - The deposition of living cells on microarray surfaces can be used to create physiologically relevant architecture in vitro. Such living cell microarrays enable the reconstruction of biological processes outside the body in a miniaturized format and have many advantages over traditional cell culture. The present protocol offers an option for the preparation and analysis of living primary and stem cell-based microarrays utilizing the standard microarray equipment (contact-free piezoelectric nanoprinter, microarray scanner), as well as microscopy. To produce living cell microarrays, we applied two kinds of mesenchymal stem cells (MSCs) isolated from umbilical cord and adipose tissue, as well as human umbilical vein endothelial cells (HUVECs) as model cells. We used live imaging microscopy for the online monitoring of cell spots in total size, staining of viable cells with Calcein acetoxymethyl ester (Calcein-AM) and treatment of MSCs with differentiation media to analyze the proliferation, viability, and differentiation potential of printed cells. This way, the general applicability of the established living cell-based microarray production was demonstrated.
AB - The deposition of living cells on microarray surfaces can be used to create physiologically relevant architecture in vitro. Such living cell microarrays enable the reconstruction of biological processes outside the body in a miniaturized format and have many advantages over traditional cell culture. The present protocol offers an option for the preparation and analysis of living primary and stem cell-based microarrays utilizing the standard microarray equipment (contact-free piezoelectric nanoprinter, microarray scanner), as well as microscopy. To produce living cell microarrays, we applied two kinds of mesenchymal stem cells (MSCs) isolated from umbilical cord and adipose tissue, as well as human umbilical vein endothelial cells (HUVECs) as model cells. We used live imaging microscopy for the online monitoring of cell spots in total size, staining of viable cells with Calcein acetoxymethyl ester (Calcein-AM) and treatment of MSCs with differentiation media to analyze the proliferation, viability, and differentiation potential of printed cells. This way, the general applicability of the established living cell-based microarray production was demonstrated.
KW - Endothelial cells
KW - Living cell microarrays
KW - Mesenchymal stem cells
KW - Microarray technology
KW - Online monitoring microscopy
KW - Piezoelectric nanoprinting
KW - Primary human cells
UR - http://www.scopus.com/inward/record.url?scp=85045416339&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7792-5_11
DO - 10.1007/978-1-4939-7792-5_11
M3 - Contribution to book/anthology
C2 - 29633210
AN - SCOPUS:85045416339
SN - 978-1-4939-7791-8
T3 - Methods in Molecular Biology
SP - 131
EP - 145
BT - Cell-Based Microarrays
PB - Springer Verlag
ER -