Mycoremediation affects antioxidative status in winter rye plants grown at Chernobyl exclusion zone site in Ukraine

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Ana Vuković
  • Wolfgang Schulz
  • Ivna Štolfa Čamagajevac
  • Apoorva Gaur
  • Clemens Walther
  • Dharmendra K. Gupta

External Research Organisations

  • Josip Juraj Strossmayer University of Osijek
  • Ministry of Environment, Forest and Climate Change
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Details

Original languageEnglish
Pages (from-to)25818-25827
Number of pages10
JournalEnvironmental Science and Pollution Research
Volume27
Issue number20
Early online date13 May 2020
Publication statusPublished - Jul 2020

Abstract

Soil contaminated with heavy metals in general and radionuclides in particular represents an escalating problem for all living organisms. Since, Chernobyl nuclear power plant accident in 1986 in Ukraine, an exclusion zone of 30 km around the former power plant is uninhabitable land due to severe contamination. Two most notable beta emitters contributing to dose hazards for decades is radioactive 137Cs/90Sr. However, large parts of the zone are also highly contaminated with uranium particles (hot particles) bearing trace amounts of highly alpha-emitting radionuclides. We established an experiment at exclusion zone with the aim to investigate the influence of two macro fungi (Schizophyllum commune (S.C.) and Leucoagaricus naucinus (L.N.)) on oxidative status and antioxidative responses in winter rye plants; from this, we wanted to test the radionuclide/heavy metals retention capacity of both fungi, and probe their further potential for mycoremediation. Result shows some differences in the concentrations of radionuclides/heavy metals and micro/macronutrients uptake in plants. As a biomarker of oxidative status, lipid peroxidation (LPO) levels and other antioxidative parameters were determined, i.e., superoxide-dismutase (SOD) isoenzymes, cysteine (CYS), and ascorbic acid (AA) concentrations as well as catalase (CAT) and glutathione reductase (GR) activities in winter rye shoots. LPO showed no significant differences between controls and plants cultivated with macro fungi. However, CAT activities were elevated in the presence of S.C/L.N compared with control, while GR activity was significantly higher only in presence of S.C. In contrast, isozyme of SOD (Cu,Zn-SOD) was the most prominent in control. Likewise, CYS content was lower in plants grown with both fungi, while AA concentration was only lower in the presence of L.N. The results showed that presence of fungi in radionuclide contaminated soil caused induction of antioxidative response in shoots of winter rye and that the response depended on the type of fungi used.

Keywords

    Chernobyl exclusion zone, Leucoagaricus naucinus, Mycoremediation, Oxidative stress, Schizophyllum commune, Winter rye plant

ASJC Scopus subject areas

Cite this

Mycoremediation affects antioxidative status in winter rye plants grown at Chernobyl exclusion zone site in Ukraine. / Vuković, Ana; Schulz, Wolfgang; Čamagajevac, Ivna Štolfa et al.
In: Environmental Science and Pollution Research, Vol. 27, No. 20, 07.2020, p. 25818-25827.

Research output: Contribution to journalArticleResearchpeer review

Vuković A, Schulz W, Čamagajevac IŠ, Gaur A, Walther C, Gupta DK. Mycoremediation affects antioxidative status in winter rye plants grown at Chernobyl exclusion zone site in Ukraine. Environmental Science and Pollution Research. 2020 Jul;27(20):25818-25827. Epub 2020 May 13. doi: 10.1007/s11356-020-09137-w
Vuković, Ana ; Schulz, Wolfgang ; Čamagajevac, Ivna Štolfa et al. / Mycoremediation affects antioxidative status in winter rye plants grown at Chernobyl exclusion zone site in Ukraine. In: Environmental Science and Pollution Research. 2020 ; Vol. 27, No. 20. pp. 25818-25827.
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title = "Mycoremediation affects antioxidative status in winter rye plants grown at Chernobyl exclusion zone site in Ukraine",
abstract = "Soil contaminated with heavy metals in general and radionuclides in particular represents an escalating problem for all living organisms. Since, Chernobyl nuclear power plant accident in 1986 in Ukraine, an exclusion zone of 30 km around the former power plant is uninhabitable land due to severe contamination. Two most notable beta emitters contributing to dose hazards for decades is radioactive 137Cs/90Sr. However, large parts of the zone are also highly contaminated with uranium particles (hot particles) bearing trace amounts of highly alpha-emitting radionuclides. We established an experiment at exclusion zone with the aim to investigate the influence of two macro fungi (Schizophyllum commune (S.C.) and Leucoagaricus naucinus (L.N.)) on oxidative status and antioxidative responses in winter rye plants; from this, we wanted to test the radionuclide/heavy metals retention capacity of both fungi, and probe their further potential for mycoremediation. Result shows some differences in the concentrations of radionuclides/heavy metals and micro/macronutrients uptake in plants. As a biomarker of oxidative status, lipid peroxidation (LPO) levels and other antioxidative parameters were determined, i.e., superoxide-dismutase (SOD) isoenzymes, cysteine (CYS), and ascorbic acid (AA) concentrations as well as catalase (CAT) and glutathione reductase (GR) activities in winter rye shoots. LPO showed no significant differences between controls and plants cultivated with macro fungi. However, CAT activities were elevated in the presence of S.C/L.N compared with control, while GR activity was significantly higher only in presence of S.C. In contrast, isozyme of SOD (Cu,Zn-SOD) was the most prominent in control. Likewise, CYS content was lower in plants grown with both fungi, while AA concentration was only lower in the presence of L.N. The results showed that presence of fungi in radionuclide contaminated soil caused induction of antioxidative response in shoots of winter rye and that the response depended on the type of fungi used.",
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AU - Vuković, Ana

AU - Schulz, Wolfgang

AU - Čamagajevac, Ivna Štolfa

AU - Gaur, Apoorva

AU - Walther, Clemens

AU - Gupta, Dharmendra K.

N1 - Funding Information: The authors greatly acknowledge Dr. Johannes Raff of Institute for Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany, for providing two different macro fungi for the experiments. Authors also acknowledge Dr. Sergiy Dubchak, State Specialized Enterprise “Radon,” Kyiv, Ukraine, and Dr. Igor Chyzhevsky, State Specialized Enterprise “Eco-center,” Chornobyl, Ukraine, for their help during this experiment and in access of Chornobyl exclusion zone.

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