Details
| Original language | English |
|---|---|
| Article number | 129 |
| Journal | Archives of microbiology |
| Volume | 205 |
| Issue number | 4 |
| Publication status | Published - 22 Mar 2023 |
Abstract
Since the first report in 2009, at least ten additional viruses have been identified and assigned to the proposed virus family Alternaviridae. Here we report two new mycoviruses tentatively assigned to this family, both identified as members of the fungal family Nectriaceae, which were isolated from surface-disinfected apple roots (Malus x domestica, Borkh.) affected by apple replant disease (ARD). ARD is a highly complex, worldwide-occurring disease resulting from plant reactions to a disturbed (micro)-biome and leads to high economic losses every year. The first alternavirus characterized in this study was identified in a Dactylonectria torresensis isolate. The virus was tentatively named dactylonectria torresensis alternavirus 1 (DtAV1) as the first member of the proposed new species Alternavirus dactylonectriae. The second virus was identified in an isolate of Ilyonectria robusta and was tentatively named ilyonectria robusta alternavirus 1 (IrAV1) as the first member of the proposed new species Alternavirus ilyonectriae. Full genomic sequences of the viruses were determined and are presented. Further, we found hints for putative components of a methyl transferase machinery using in silico approaches. This putative protein domain is encoded by segment 2. However, this result only establishes the basis for subsequent studies in which the function must be confirmed experimentally in vitro. Thus, this is the first study where a function is predicted to all three genomic segments within the group of the alternaviruses. These findings provide further insights into the virome of ARD-associated fungi and are therefore another brick in the wall of understanding the complexity of the disease.
Keywords
- Alternavirus, Apple replant disease, Dactylonectria torresensis, dsRNA, Ilyonectria robusta, Mycovirus, RNA capping
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Archives of microbiology, Vol. 205, No. 4, 129, 22.03.2023.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Molecular characterization of two new alternaviruses identified in members of the fungal family Nectriaceae
AU - Pielhop, Tom P.
AU - Popp, Carolin
AU - Fricke, Sebastian
AU - Knierim, Dennis
AU - Margaria, Paolo
AU - Maiß, Edgar
N1 - Funding Information: This work was funded by the Federal Ministry of Education and Research and is part of the ORDIAmur network (FKZ: 031B0025A, 031B0512), which is allocated to the BONARES program. We thank Mr. Aaron Balke for his assistance with dsRNA extractions. Open Access funding enabled and organized by Projekt DEAL. This work was funded by the Federal Ministry of Education and Research and is part of the ORDIAmur network (FKZ: 031B0025A, 031B0512), which is allocated to the BONARES program.
PY - 2023/3/22
Y1 - 2023/3/22
N2 - Since the first report in 2009, at least ten additional viruses have been identified and assigned to the proposed virus family Alternaviridae. Here we report two new mycoviruses tentatively assigned to this family, both identified as members of the fungal family Nectriaceae, which were isolated from surface-disinfected apple roots (Malus x domestica, Borkh.) affected by apple replant disease (ARD). ARD is a highly complex, worldwide-occurring disease resulting from plant reactions to a disturbed (micro)-biome and leads to high economic losses every year. The first alternavirus characterized in this study was identified in a Dactylonectria torresensis isolate. The virus was tentatively named dactylonectria torresensis alternavirus 1 (DtAV1) as the first member of the proposed new species Alternavirus dactylonectriae. The second virus was identified in an isolate of Ilyonectria robusta and was tentatively named ilyonectria robusta alternavirus 1 (IrAV1) as the first member of the proposed new species Alternavirus ilyonectriae. Full genomic sequences of the viruses were determined and are presented. Further, we found hints for putative components of a methyl transferase machinery using in silico approaches. This putative protein domain is encoded by segment 2. However, this result only establishes the basis for subsequent studies in which the function must be confirmed experimentally in vitro. Thus, this is the first study where a function is predicted to all three genomic segments within the group of the alternaviruses. These findings provide further insights into the virome of ARD-associated fungi and are therefore another brick in the wall of understanding the complexity of the disease.
AB - Since the first report in 2009, at least ten additional viruses have been identified and assigned to the proposed virus family Alternaviridae. Here we report two new mycoviruses tentatively assigned to this family, both identified as members of the fungal family Nectriaceae, which were isolated from surface-disinfected apple roots (Malus x domestica, Borkh.) affected by apple replant disease (ARD). ARD is a highly complex, worldwide-occurring disease resulting from plant reactions to a disturbed (micro)-biome and leads to high economic losses every year. The first alternavirus characterized in this study was identified in a Dactylonectria torresensis isolate. The virus was tentatively named dactylonectria torresensis alternavirus 1 (DtAV1) as the first member of the proposed new species Alternavirus dactylonectriae. The second virus was identified in an isolate of Ilyonectria robusta and was tentatively named ilyonectria robusta alternavirus 1 (IrAV1) as the first member of the proposed new species Alternavirus ilyonectriae. Full genomic sequences of the viruses were determined and are presented. Further, we found hints for putative components of a methyl transferase machinery using in silico approaches. This putative protein domain is encoded by segment 2. However, this result only establishes the basis for subsequent studies in which the function must be confirmed experimentally in vitro. Thus, this is the first study where a function is predicted to all three genomic segments within the group of the alternaviruses. These findings provide further insights into the virome of ARD-associated fungi and are therefore another brick in the wall of understanding the complexity of the disease.
KW - Alternavirus
KW - Apple replant disease
KW - Dactylonectria torresensis
KW - dsRNA
KW - Ilyonectria robusta
KW - Mycovirus
KW - RNA capping
UR - http://www.scopus.com/inward/record.url?scp=85150840597&partnerID=8YFLogxK
U2 - 10.1007/s00203-023-03477-0
DO - 10.1007/s00203-023-03477-0
M3 - Article
C2 - 36947239
AN - SCOPUS:85150840597
VL - 205
JO - Archives of microbiology
JF - Archives of microbiology
SN - 0302-8933
IS - 4
M1 - 129
ER -