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Long-Term Neurological and Behavioral Results of Biodegradable Scaffold Implantation in Mice Brain

Research output: Contribution to journal › Article › Research › peer review

Authors

  • A. V. Balyabin
  • Olga P. Tikhobrazova
  • M. S. Muravyeva
  • E. A. Klyuev
  • A. V. Koroleva
  • B. N. Chichkov

External Research Organisations

  • Russian Ministry of Health
  • Nizhny Novgorod State Medical Acad.
  • Nizhni Novgorod State University
  • Institute of Photon Technologies of Federal Scientific Research Centre “Crystallography and Photonics” of the Russian Academy of Sciences
  • Laser Zentrum Hannover e.V. (LZH)
  • University of Galway
  • Sechenov First Moscow State Medical University

Details

Original languageEnglish
Pages (from-to)198-209
Number of pages12
JournalSovremennye tehnologii v medicine
Volume8
Issue number4
Publication statusPublished - 1 Jan 2016
Externally publishedYes

Abstract

The aim of the study was to assess the integral outcomes of implantation biocompatible scaffold as the carrier of neural stem cells in the reconstructive surgery of open traumatic brain injury (TBI) by parameters of neurological and cognitive status of the animals in the experiment. Materials and Methods. Adult male C57BL/6 mice were injured with open-skull weight-drop method. 3D hydrogel scaffold based on modified chitosan in complex with hyaluronic acid was transplanted into the lesion cavity 1 week after TBI. Using a variety of behavioral and cognitive tests (modified neurological severity scores (mNSS), open field test as well as novel object recognition and passive avoidance tests) the short-and long-term neurological and memory functions sequelae induced by TBI were assessed. Magnetic resonance imaging was used to visualize the injury site. Results. Significant functional recovery was observed on both the mNSS and open field tests in the scaffold transplantation group compared to the control TBI group. In addition, enhanced improvement of short and long-term memory functions was found 5 months post injury. Magnetic resonance imaging data revealed that the scaffold transplantation result in decreasing of the volume of injury area compared to control TBI group and preventing the disruption of the neural networks of the brain. Conclusion. Taken together, our findings indicated that transplantation of 3D biodegradable scaffold into injury cavity contributed to the preservation of volume in the damaged region in the first months after TBI which in turn led to a better functional recovery in the remote period.

Keywords

    Biodegradable scaffolds, Functional recovery, Traumatic brain injury

ASJC Scopus subject areas

Cite this

Long-Term Neurological and Behavioral Results of Biodegradable Scaffold Implantation in Mice Brain. / Balyabin, A. V.; Tikhobrazova, Olga P.; Muravyeva, M. S. et al.
In: Sovremennye tehnologii v medicine, Vol. 8, No. 4, 01.01.2016, p. 198-209.

Research output: Contribution to journal › Article › Research › peer review

Balyabin, AV, Tikhobrazova, OP, Muravyeva, MS, Klyuev, EA, Ponyatovskaya, AV, Shirokova, OM, Bardakova, KN, Minaev, NV, Koroleva, AV, Mitaeva, YI, Mitroshina, EV, Vedunova, MV, Rochev, YA, Chichkov, BN, Timashev, PS, Bagratashvili, VN & Mukhina, IV 2016, 'Long-Term Neurological and Behavioral Results of Biodegradable Scaffold Implantation in Mice Brain', Sovremennye tehnologii v medicine, vol. 8, no. 4, pp. 198-209. https://doi.org/10.17691/stm2016.8.4.25
Balyabin, A. V., Tikhobrazova, O. P., Muravyeva, M. S., Klyuev, E. A., Ponyatovskaya, A. V., Shirokova, O. M., Bardakova, K. N., Minaev, N. V., Koroleva, A. V., Mitaeva, Y. I., Mitroshina, E. V., Vedunova, M. V., Rochev, Y. A., Chichkov, B. N., Timashev, P. S., Bagratashvili, V. N., & Mukhina, I. V. (2016). Long-Term Neurological and Behavioral Results of Biodegradable Scaffold Implantation in Mice Brain. Sovremennye tehnologii v medicine, 8(4), 198-209. https://doi.org/10.17691/stm2016.8.4.25
Balyabin AV, Tikhobrazova OP, Muravyeva MS, Klyuev EA, Ponyatovskaya AV, Shirokova OM et al. Long-Term Neurological and Behavioral Results of Biodegradable Scaffold Implantation in Mice Brain. Sovremennye tehnologii v medicine. 2016 Jan 1;8(4):198-209. doi: 10.17691/stm2016.8.4.25
Balyabin, A. V. ; Tikhobrazova, Olga P. ; Muravyeva, M. S. et al. / Long-Term Neurological and Behavioral Results of Biodegradable Scaffold Implantation in Mice Brain. In: Sovremennye tehnologii v medicine. 2016 ; Vol. 8, No. 4. pp. 198-209.
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title = "Long-Term Neurological and Behavioral Results of Biodegradable Scaffold Implantation in Mice Brain",
abstract = "The aim of the study was to assess the integral outcomes of implantation biocompatible scaffold as the carrier of neural stem cells in the reconstructive surgery of open traumatic brain injury (TBI) by parameters of neurological and cognitive status of the animals in the experiment. Materials and Methods. Adult male C57BL/6 mice were injured with open-skull weight-drop method. 3D hydrogel scaffold based on modified chitosan in complex with hyaluronic acid was transplanted into the lesion cavity 1 week after TBI. Using a variety of behavioral and cognitive tests (modified neurological severity scores (mNSS), open field test as well as novel object recognition and passive avoidance tests) the short-and long-term neurological and memory functions sequelae induced by TBI were assessed. Magnetic resonance imaging was used to visualize the injury site. Results. Significant functional recovery was observed on both the mNSS and open field tests in the scaffold transplantation group compared to the control TBI group. In addition, enhanced improvement of short and long-term memory functions was found 5 months post injury. Magnetic resonance imaging data revealed that the scaffold transplantation result in decreasing of the volume of injury area compared to control TBI group and preventing the disruption of the neural networks of the brain. Conclusion. Taken together, our findings indicated that transplantation of 3D biodegradable scaffold into injury cavity contributed to the preservation of volume in the damaged region in the first months after TBI which in turn led to a better functional recovery in the remote period.",
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Download

TY - JOUR

T1 - Long-Term Neurological and Behavioral Results of Biodegradable Scaffold Implantation in Mice Brain

AU - Balyabin, A. V.

AU - Tikhobrazova, Olga P.

AU - Muravyeva, M. S.

AU - Klyuev, E. A.

AU - Ponyatovskaya, A. V.

AU - Shirokova, O. M.

AU - Bardakova, K. N.

AU - Minaev, N. V.

AU - Koroleva, A. V.

AU - Mitaeva, Y. I.

AU - Mitroshina, E. V.

AU - Vedunova, M. V.

AU - Rochev, Y. A.

AU - Chichkov, B. N.

AU - Timashev, P. S.

AU - Bagratashvili, V. N.

AU - Mukhina, I. V.

N1 - Funding information: The research was supported by the Federal Target Program “Research and development in priority areas of the development of the scientific and technological complex of Russia for 2014–2020” of the Ministry of Education and Science of Russia (Project ID RFMEFI60715X0117).

PY - 2016/1/1

Y1 - 2016/1/1

N2 - The aim of the study was to assess the integral outcomes of implantation biocompatible scaffold as the carrier of neural stem cells in the reconstructive surgery of open traumatic brain injury (TBI) by parameters of neurological and cognitive status of the animals in the experiment. Materials and Methods. Adult male C57BL/6 mice were injured with open-skull weight-drop method. 3D hydrogel scaffold based on modified chitosan in complex with hyaluronic acid was transplanted into the lesion cavity 1 week after TBI. Using a variety of behavioral and cognitive tests (modified neurological severity scores (mNSS), open field test as well as novel object recognition and passive avoidance tests) the short-and long-term neurological and memory functions sequelae induced by TBI were assessed. Magnetic resonance imaging was used to visualize the injury site. Results. Significant functional recovery was observed on both the mNSS and open field tests in the scaffold transplantation group compared to the control TBI group. In addition, enhanced improvement of short and long-term memory functions was found 5 months post injury. Magnetic resonance imaging data revealed that the scaffold transplantation result in decreasing of the volume of injury area compared to control TBI group and preventing the disruption of the neural networks of the brain. Conclusion. Taken together, our findings indicated that transplantation of 3D biodegradable scaffold into injury cavity contributed to the preservation of volume in the damaged region in the first months after TBI which in turn led to a better functional recovery in the remote period.

AB - The aim of the study was to assess the integral outcomes of implantation biocompatible scaffold as the carrier of neural stem cells in the reconstructive surgery of open traumatic brain injury (TBI) by parameters of neurological and cognitive status of the animals in the experiment. Materials and Methods. Adult male C57BL/6 mice were injured with open-skull weight-drop method. 3D hydrogel scaffold based on modified chitosan in complex with hyaluronic acid was transplanted into the lesion cavity 1 week after TBI. Using a variety of behavioral and cognitive tests (modified neurological severity scores (mNSS), open field test as well as novel object recognition and passive avoidance tests) the short-and long-term neurological and memory functions sequelae induced by TBI were assessed. Magnetic resonance imaging was used to visualize the injury site. Results. Significant functional recovery was observed on both the mNSS and open field tests in the scaffold transplantation group compared to the control TBI group. In addition, enhanced improvement of short and long-term memory functions was found 5 months post injury. Magnetic resonance imaging data revealed that the scaffold transplantation result in decreasing of the volume of injury area compared to control TBI group and preventing the disruption of the neural networks of the brain. Conclusion. Taken together, our findings indicated that transplantation of 3D biodegradable scaffold into injury cavity contributed to the preservation of volume in the damaged region in the first months after TBI which in turn led to a better functional recovery in the remote period.

KW - Biodegradable scaffolds

KW - Functional recovery

KW - Traumatic brain injury

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