Details
| Original language | English |
|---|---|
| Pages (from-to) | 1203-1219 |
| Number of pages | 17 |
| Journal | Plant physiology |
| Volume | 175 |
| Issue number | 3 |
| Publication status | Published - Nov 2017 |
| Externally published | Yes |
Abstract
Plants possessing dysfunctional plastids due to defects in pigment biosynthesis or translation are known to repress photosynthesis-associated nuclear genes via retrograde signals from the disturbed organelles toward the nucleus. These signals are thought to be essential for proper biogenesis and function of the plastid. Mutants lacking plastid-encoded RNA polymerase-associated proteins (PAPs) display a genetic arrest in eoplast-chloroplast transition leading to an albino phenotype in the light. Retrograde signaling in these mutants, therefore, could be expected to be similar as under conditions inducing plastid dysfunction. To answer this question, we performed plastome- and genomewide array analyses in the pap7-1 mutant of Arabidopsis (Arabidopsis thaliana). In parallel, we determined the potential overlap with light-regulated expression networks. To this end, we performed a comparative expression profiling approach using light- and dark-grown wild-type plants as relative control for the expression profiles obtained from light-grown pap7-1 mutants. Our data indicate a specific impact of retrograde signals on metabolism-related genes in pap7-1 mutants reflecting the starvation situation of the albino seedlings. In contrast, light regulation of PhANGs and other nuclear gene groups appears to be fully functional in this mutant, indicating that a block in chloroplast biogenesis per se does not repress expression of them as suggested by earlier studies. Only genes for light harvesting complex proteins displayed a significant repression indicating an exclusive retrograde impact on this gene family. Our results indicate that chloroplasts and arrested plastids each emit specific signals that control different target gene modules both in positive and negative manner.
Keywords
- Arabidopsis/genetics, Arabidopsis Proteins/genetics, Chloroplast Proteins/genetics, Cluster Analysis, Gene Expression Profiling, Gene Expression Regulation, Plant/radiation effects, Gene Ontology, Gene Regulatory Networks, Genes, Plant, Light, Methyltransferases/genetics, Models, Biological, Morphogenesis/radiation effects, Mutation/genetics, Photosynthesis/genetics, Plastids/metabolism, RNA, Messenger/genetics, Signal Transduction/radiation effects
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant physiology, Vol. 175, No. 3, 11.2017, p. 1203-1219.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Light and Plastid Signals Regulate Different Sets of Genes in the Albino Mutant Pap7-1
AU - Grübler, Björn
AU - Merendino, Livia
AU - Twardziok, Sven O
AU - Mininno, Morgane
AU - Allorent, Guillaume
AU - Chevalier, Fabien
AU - Liebers, Monique
AU - Blanvillain, Robert
AU - Mayer, Klaus F X
AU - Lerbs-Mache, Silva
AU - Ravanel, Stéphane
AU - Pfannschmidt, Thomas
N1 - Funding information: 1This work was supported by grants from the Deutsche For-schungsgemeinschaft (DFG) to T.P. (PF323-5-2) and the Deutsche Forschungsgemeinschaft (DFG) research group FOR 804. The study also received institutional support from the French National Research Agency (ANR-10-LABEX-04 GRAL Labex, Grenoble Alliance for Integrated Structural Cell Biology).
PY - 2017/11
Y1 - 2017/11
N2 - Plants possessing dysfunctional plastids due to defects in pigment biosynthesis or translation are known to repress photosynthesis-associated nuclear genes via retrograde signals from the disturbed organelles toward the nucleus. These signals are thought to be essential for proper biogenesis and function of the plastid. Mutants lacking plastid-encoded RNA polymerase-associated proteins (PAPs) display a genetic arrest in eoplast-chloroplast transition leading to an albino phenotype in the light. Retrograde signaling in these mutants, therefore, could be expected to be similar as under conditions inducing plastid dysfunction. To answer this question, we performed plastome- and genomewide array analyses in the pap7-1 mutant of Arabidopsis (Arabidopsis thaliana). In parallel, we determined the potential overlap with light-regulated expression networks. To this end, we performed a comparative expression profiling approach using light- and dark-grown wild-type plants as relative control for the expression profiles obtained from light-grown pap7-1 mutants. Our data indicate a specific impact of retrograde signals on metabolism-related genes in pap7-1 mutants reflecting the starvation situation of the albino seedlings. In contrast, light regulation of PhANGs and other nuclear gene groups appears to be fully functional in this mutant, indicating that a block in chloroplast biogenesis per se does not repress expression of them as suggested by earlier studies. Only genes for light harvesting complex proteins displayed a significant repression indicating an exclusive retrograde impact on this gene family. Our results indicate that chloroplasts and arrested plastids each emit specific signals that control different target gene modules both in positive and negative manner.
AB - Plants possessing dysfunctional plastids due to defects in pigment biosynthesis or translation are known to repress photosynthesis-associated nuclear genes via retrograde signals from the disturbed organelles toward the nucleus. These signals are thought to be essential for proper biogenesis and function of the plastid. Mutants lacking plastid-encoded RNA polymerase-associated proteins (PAPs) display a genetic arrest in eoplast-chloroplast transition leading to an albino phenotype in the light. Retrograde signaling in these mutants, therefore, could be expected to be similar as under conditions inducing plastid dysfunction. To answer this question, we performed plastome- and genomewide array analyses in the pap7-1 mutant of Arabidopsis (Arabidopsis thaliana). In parallel, we determined the potential overlap with light-regulated expression networks. To this end, we performed a comparative expression profiling approach using light- and dark-grown wild-type plants as relative control for the expression profiles obtained from light-grown pap7-1 mutants. Our data indicate a specific impact of retrograde signals on metabolism-related genes in pap7-1 mutants reflecting the starvation situation of the albino seedlings. In contrast, light regulation of PhANGs and other nuclear gene groups appears to be fully functional in this mutant, indicating that a block in chloroplast biogenesis per se does not repress expression of them as suggested by earlier studies. Only genes for light harvesting complex proteins displayed a significant repression indicating an exclusive retrograde impact on this gene family. Our results indicate that chloroplasts and arrested plastids each emit specific signals that control different target gene modules both in positive and negative manner.
KW - Arabidopsis/genetics
KW - Arabidopsis Proteins/genetics
KW - Chloroplast Proteins/genetics
KW - Cluster Analysis
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Plant/radiation effects
KW - Gene Ontology
KW - Gene Regulatory Networks
KW - Genes, Plant
KW - Light
KW - Methyltransferases/genetics
KW - Models, Biological
KW - Morphogenesis/radiation effects
KW - Mutation/genetics
KW - Photosynthesis/genetics
KW - Plastids/metabolism
KW - RNA, Messenger/genetics
KW - Signal Transduction/radiation effects
UR - http://www.scopus.com/inward/record.url?scp=85033796414&partnerID=8YFLogxK
U2 - 10.1104/pp.17.00982
DO - 10.1104/pp.17.00982
M3 - Article
C2 - 28935841
VL - 175
SP - 1203
EP - 1219
JO - Plant physiology
JF - Plant physiology
SN - 0032-0889
IS - 3
ER -