Loading [MathJax]/extensions/tex2jax.js

Iterative Cellular Screening System for Nanoparticle Safety Testing

Research output: Contribution to journalArticleResearchpeer review

Authors

Research Organisations

External Research Organisations

  • Technion-Israel Institute of Technology
  • University of Natural Resources and Applied Life Sciences (BOKU)
  • Saint Petersburg State University

Details

Original languageEnglish
Article number691069
JournalJournal of nanomaterials
Volume2015
Publication statusPublished - 19 Aug 2015

Abstract

Nanoparticles have the potential to exhibit risks to human beings and to the environment; due to the wide applications of nanoproducts, extensive risk management must not be neglected. Therefore, we have constructed a cell-based, iterative screening system to examine a variety of nanoproducts concerning their toxicity during development. The sensitivity and application of various cell-based methods were discussed and proven by applying the screening to two different nanoparticles: zinc oxide and titanium dioxide nanoparticles. They were used as benchmarks to set up our methods and to examine their effects on mammalian cell lines. Different biological processes such as cell viability, gene expression of interleukin-8 and heat shock protein 70, as well as morphology changes were investigated. Within our screening system, both nanoparticle suspensions and coatings can be tested. Electric cell impedance measurements revealed to be a good method for online monitoring of cellular behavior. The implementation of three-dimensional cell culture is essential to better mimic in vivo conditions. In conclusion, our screening system is highly efficient, cost minimizing, and reduces the need for animal studies.

ASJC Scopus subject areas

Cite this

Iterative Cellular Screening System for Nanoparticle Safety Testing. / Sambale, Franziska; Stahl, Frank; Rüdinger, Ferdinand et al.
In: Journal of nanomaterials, Vol. 2015, 691069, 19.08.2015.

Research output: Contribution to journalArticleResearchpeer review

Sambale, F., Stahl, F., Rüdinger, F., Seliktar, D., Kasper, C., Bahnemann, D., & Scheper, T. (2015). Iterative Cellular Screening System for Nanoparticle Safety Testing. Journal of nanomaterials, 2015, Article 691069. https://doi.org/10.1155/2015/691069
Sambale F, Stahl F, Rüdinger F, Seliktar D, Kasper C, Bahnemann D et al. Iterative Cellular Screening System for Nanoparticle Safety Testing. Journal of nanomaterials. 2015 Aug 19;2015:691069. doi: 10.1155/2015/691069
Sambale, Franziska ; Stahl, Frank ; Rüdinger, Ferdinand et al. / Iterative Cellular Screening System for Nanoparticle Safety Testing. In: Journal of nanomaterials. 2015 ; Vol. 2015.
Download
@article{a597eedc1f3f4333be6b214b11374273,
title = "Iterative Cellular Screening System for Nanoparticle Safety Testing",
abstract = "Nanoparticles have the potential to exhibit risks to human beings and to the environment; due to the wide applications of nanoproducts, extensive risk management must not be neglected. Therefore, we have constructed a cell-based, iterative screening system to examine a variety of nanoproducts concerning their toxicity during development. The sensitivity and application of various cell-based methods were discussed and proven by applying the screening to two different nanoparticles: zinc oxide and titanium dioxide nanoparticles. They were used as benchmarks to set up our methods and to examine their effects on mammalian cell lines. Different biological processes such as cell viability, gene expression of interleukin-8 and heat shock protein 70, as well as morphology changes were investigated. Within our screening system, both nanoparticle suspensions and coatings can be tested. Electric cell impedance measurements revealed to be a good method for online monitoring of cellular behavior. The implementation of three-dimensional cell culture is essential to better mimic in vivo conditions. In conclusion, our screening system is highly efficient, cost minimizing, and reduces the need for animal studies.",
author = "Franziska Sambale and Frank Stahl and Ferdinand R{\"u}dinger and Dror Seliktar and Cornelia Kasper and Detlef Bahnemann and Thomas Scheper",
year = "2015",
month = aug,
day = "19",
doi = "10.1155/2015/691069",
language = "English",
volume = "2015",
journal = "Journal of nanomaterials",
issn = "1687-4110",
publisher = "John Wiley and Sons Ltd",

}

Download

TY - JOUR

T1 - Iterative Cellular Screening System for Nanoparticle Safety Testing

AU - Sambale, Franziska

AU - Stahl, Frank

AU - Rüdinger, Ferdinand

AU - Seliktar, Dror

AU - Kasper, Cornelia

AU - Bahnemann, Detlef

AU - Scheper, Thomas

PY - 2015/8/19

Y1 - 2015/8/19

N2 - Nanoparticles have the potential to exhibit risks to human beings and to the environment; due to the wide applications of nanoproducts, extensive risk management must not be neglected. Therefore, we have constructed a cell-based, iterative screening system to examine a variety of nanoproducts concerning their toxicity during development. The sensitivity and application of various cell-based methods were discussed and proven by applying the screening to two different nanoparticles: zinc oxide and titanium dioxide nanoparticles. They were used as benchmarks to set up our methods and to examine their effects on mammalian cell lines. Different biological processes such as cell viability, gene expression of interleukin-8 and heat shock protein 70, as well as morphology changes were investigated. Within our screening system, both nanoparticle suspensions and coatings can be tested. Electric cell impedance measurements revealed to be a good method for online monitoring of cellular behavior. The implementation of three-dimensional cell culture is essential to better mimic in vivo conditions. In conclusion, our screening system is highly efficient, cost minimizing, and reduces the need for animal studies.

AB - Nanoparticles have the potential to exhibit risks to human beings and to the environment; due to the wide applications of nanoproducts, extensive risk management must not be neglected. Therefore, we have constructed a cell-based, iterative screening system to examine a variety of nanoproducts concerning their toxicity during development. The sensitivity and application of various cell-based methods were discussed and proven by applying the screening to two different nanoparticles: zinc oxide and titanium dioxide nanoparticles. They were used as benchmarks to set up our methods and to examine their effects on mammalian cell lines. Different biological processes such as cell viability, gene expression of interleukin-8 and heat shock protein 70, as well as morphology changes were investigated. Within our screening system, both nanoparticle suspensions and coatings can be tested. Electric cell impedance measurements revealed to be a good method for online monitoring of cellular behavior. The implementation of three-dimensional cell culture is essential to better mimic in vivo conditions. In conclusion, our screening system is highly efficient, cost minimizing, and reduces the need for animal studies.

UR - http://www.scopus.com/inward/record.url?scp=84940729797&partnerID=8YFLogxK

U2 - 10.1155/2015/691069

DO - 10.1155/2015/691069

M3 - Article

AN - SCOPUS:84940729797

VL - 2015

JO - Journal of nanomaterials

JF - Journal of nanomaterials

SN - 1687-4110

M1 - 691069

ER -

By the same author(s)