Details
| Original language | English |
|---|---|
| Pages (from-to) | 3219-3225 |
| Number of pages | 7 |
| Journal | CHEMSUSCHEM |
| Volume | 14 |
| Issue number | 15 |
| Early online date | 17 Jun 2021 |
| Publication status | Published - 11 Aug 2021 |
| Externally published | Yes |
Abstract
Cyanobacteria have the capacity to use photosynthesis to fuel their metabolism, which makes them highly promising production systems for the sustainable production of chemicals. Yet, their dependency on visible light limits the cell-density, which is a challenge for the scale-up. Here, it was shown with the example of a light-dependent biotransformation that internal illumination in a bubble column reactor equipped with wireless light emitters (WLEs) could overcome this limitation. Cells of the cyanobacterium Synechocystis sp. PCC 6803 expressing the gene of the ene-reductase YqjM were used for the reduction of 2-methylmaleimide to (R)-2-methylsuccinimide with high optical purity (>99 % ee). Compared to external source of light, illumination by floating wireless light emitters allowed a more than two-fold rate increase. Under optimized conditions, product formation rates up to 3.7 mm h−1 and specific activities of up to 65.5 U gDCW−1 were obtained, allowing the reduction of 40 mm 2-methylmaleimide with 650 mg isolated enantiopure product (73 % yield). The results demonstrate the principle of internal illumination as a means to overcome the intrinsic cell density limitation of cyanobacterial biotransformations, obtaining high reaction rates in a scalable photobioreactor.
Keywords
- asymmetric catalysis, biocatalysis, cyanobacteria, ene-reduction, photocatalysis
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Chemistry
- Chemical Engineering(all)
- General Chemical Engineering
- Materials Science(all)
- General Materials Science
- Energy(all)
- General Energy
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In: CHEMSUSCHEM, Vol. 14, No. 15, 11.08.2021, p. 3219-3225.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Internal Illumination to Overcome the Cell Density Limitation in the Scale-up of Whole-Cell Photobiocatalysis
AU - Hobisch, Markus
AU - Spasic, Jelena
AU - Malihan-Yap, Lenny
AU - Barone, Giovanni Davide
AU - Castiglione, Kathrin
AU - Tamagnini, Paula
AU - Kara, Selin
AU - Kourist, Robert
N1 - Funding Information: This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska‐Curie grant agreement No 764920 (MSCA‐EJD PhotoBioCat) and the FET Open grant agreement 899576 (Futuroleaf).
PY - 2021/8/11
Y1 - 2021/8/11
N2 - Cyanobacteria have the capacity to use photosynthesis to fuel their metabolism, which makes them highly promising production systems for the sustainable production of chemicals. Yet, their dependency on visible light limits the cell-density, which is a challenge for the scale-up. Here, it was shown with the example of a light-dependent biotransformation that internal illumination in a bubble column reactor equipped with wireless light emitters (WLEs) could overcome this limitation. Cells of the cyanobacterium Synechocystis sp. PCC 6803 expressing the gene of the ene-reductase YqjM were used for the reduction of 2-methylmaleimide to (R)-2-methylsuccinimide with high optical purity (>99 % ee). Compared to external source of light, illumination by floating wireless light emitters allowed a more than two-fold rate increase. Under optimized conditions, product formation rates up to 3.7 mm h−1 and specific activities of up to 65.5 U gDCW−1 were obtained, allowing the reduction of 40 mm 2-methylmaleimide with 650 mg isolated enantiopure product (73 % yield). The results demonstrate the principle of internal illumination as a means to overcome the intrinsic cell density limitation of cyanobacterial biotransformations, obtaining high reaction rates in a scalable photobioreactor.
AB - Cyanobacteria have the capacity to use photosynthesis to fuel their metabolism, which makes them highly promising production systems for the sustainable production of chemicals. Yet, their dependency on visible light limits the cell-density, which is a challenge for the scale-up. Here, it was shown with the example of a light-dependent biotransformation that internal illumination in a bubble column reactor equipped with wireless light emitters (WLEs) could overcome this limitation. Cells of the cyanobacterium Synechocystis sp. PCC 6803 expressing the gene of the ene-reductase YqjM were used for the reduction of 2-methylmaleimide to (R)-2-methylsuccinimide with high optical purity (>99 % ee). Compared to external source of light, illumination by floating wireless light emitters allowed a more than two-fold rate increase. Under optimized conditions, product formation rates up to 3.7 mm h−1 and specific activities of up to 65.5 U gDCW−1 were obtained, allowing the reduction of 40 mm 2-methylmaleimide with 650 mg isolated enantiopure product (73 % yield). The results demonstrate the principle of internal illumination as a means to overcome the intrinsic cell density limitation of cyanobacterial biotransformations, obtaining high reaction rates in a scalable photobioreactor.
KW - asymmetric catalysis
KW - biocatalysis
KW - cyanobacteria
KW - ene-reduction
KW - photocatalysis
UR - http://www.scopus.com/inward/record.url?scp=85109184075&partnerID=8YFLogxK
U2 - 10.1002/cssc.202100832
DO - 10.1002/cssc.202100832
M3 - Article
C2 - 34138524
AN - SCOPUS:85109184075
VL - 14
SP - 3219
EP - 3225
JO - CHEMSUSCHEM
JF - CHEMSUSCHEM
SN - 1864-5631
IS - 15
ER -