Details
| Original language | English |
|---|---|
| Article number | e202200482 |
| Journal | CHEMBIOCHEM |
| Volume | 23 |
| Issue number | 23 |
| Early online date | 12 Oct 2022 |
| Publication status | Published - 5 Dec 2022 |
Abstract
Since its discovery in 2017, the fatty acid decarboxylase (FAP) photoenzyme has been the focus of extensive research, given its ability to convert fatty acids into alka(e)nes using merely visible blue light. Unfortunately, there are still some drawbacks that limit the applicability of this biocatalyst, such as poor solubility of the substrates in aqueous media, poor photostability, and the impossibility of reusing the catalyst for several cycles. In this work, we demonstrate the use of FAP in non-conventional media as a free enzyme and an immobilized preparation. Namely, its applicability in deep eutectic solvents (DESs) and a proof-of-concept immobilization using a commercial His-tag selective carrier, a thorough study of reaction and immobilization conditions in each case, as well as reusability studies are shown. We observed an almost complete selectivity of the enzyme towards C18 decarboxylation over C16 when used in a DES, with a product analytical yield up to 81 % when using whole cells. Furthermore, when applying the immobilized enzyme in DES, we obtained yields >10-fold higher than the ones obtained in aqueous media.
Keywords
- deep eutectic solvents, drop-in biofuels, fatty acid, immobilization, photobiocatalysis
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Chemistry(all)
- Organic Chemistry
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In: CHEMBIOCHEM, Vol. 23, No. 23, e202200482, 05.12.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Immobilization and Application of Fatty Acid Photodecarboxylase in Deep Eutectic Solvents
AU - Chanquia, Santiago Nahuel
AU - Benfeldt, Frederik Vig
AU - Petrovai, Noémi
AU - Santner, Paul
AU - Hollmann, Frank
AU - Eser, Bekir Engin
AU - Kara, Selin
N1 - Funding Information: The authors thank the Novo Nordisk Foundation, Light‐BioFuels project, grant No. NNF19OC0057522. The authors are grateful to Dr. Karim Engelmark Cassimjee (EnginZyme A.B., Sweden) for providing free immobilization carrier samples.
PY - 2022/12/5
Y1 - 2022/12/5
N2 - Since its discovery in 2017, the fatty acid decarboxylase (FAP) photoenzyme has been the focus of extensive research, given its ability to convert fatty acids into alka(e)nes using merely visible blue light. Unfortunately, there are still some drawbacks that limit the applicability of this biocatalyst, such as poor solubility of the substrates in aqueous media, poor photostability, and the impossibility of reusing the catalyst for several cycles. In this work, we demonstrate the use of FAP in non-conventional media as a free enzyme and an immobilized preparation. Namely, its applicability in deep eutectic solvents (DESs) and a proof-of-concept immobilization using a commercial His-tag selective carrier, a thorough study of reaction and immobilization conditions in each case, as well as reusability studies are shown. We observed an almost complete selectivity of the enzyme towards C18 decarboxylation over C16 when used in a DES, with a product analytical yield up to 81 % when using whole cells. Furthermore, when applying the immobilized enzyme in DES, we obtained yields >10-fold higher than the ones obtained in aqueous media.
AB - Since its discovery in 2017, the fatty acid decarboxylase (FAP) photoenzyme has been the focus of extensive research, given its ability to convert fatty acids into alka(e)nes using merely visible blue light. Unfortunately, there are still some drawbacks that limit the applicability of this biocatalyst, such as poor solubility of the substrates in aqueous media, poor photostability, and the impossibility of reusing the catalyst for several cycles. In this work, we demonstrate the use of FAP in non-conventional media as a free enzyme and an immobilized preparation. Namely, its applicability in deep eutectic solvents (DESs) and a proof-of-concept immobilization using a commercial His-tag selective carrier, a thorough study of reaction and immobilization conditions in each case, as well as reusability studies are shown. We observed an almost complete selectivity of the enzyme towards C18 decarboxylation over C16 when used in a DES, with a product analytical yield up to 81 % when using whole cells. Furthermore, when applying the immobilized enzyme in DES, we obtained yields >10-fold higher than the ones obtained in aqueous media.
KW - deep eutectic solvents
KW - drop-in biofuels
KW - fatty acid
KW - immobilization
KW - photobiocatalysis
UR - http://www.scopus.com/inward/record.url?scp=85141784626&partnerID=8YFLogxK
U2 - 10.1002/cbic.202200482
DO - 10.1002/cbic.202200482
M3 - Article
VL - 23
JO - CHEMBIOCHEM
JF - CHEMBIOCHEM
SN - 1439-4227
IS - 23
M1 - e202200482
ER -