Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS

Research output: Contribution to journal › Article › Research › peer review

Authors

  • Marcus Mandel
  • Linus Holtmann
  • Manuel Raiwa
  • Annika Wunnenberg-Gust
  • Beate Riebe
  • Clemens Walther

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Details

Original languageEnglish
Article number127143
Number of pages9
JournalJournal of hazardous materials
Volume423
Early online date6 Sept 2021
Publication statusPublished - 5 Feb 2022

Abstract

In radioecological studies, there is a significant need for understanding the plant uptake of radionuclides on a cellular level. The present work applies mass spectrometry to image the radionuclide distribution within the cellular structures of plants at varying concentrations. In a first step, plants of Daucus carota and Pisum sativum labelled with iodine and rhenium were examined, at concentrations in the range of 10 mM. Cross sections of several plant parts were imaged by secondary ion mass spectrometry (SIMS) after cryogenation in order to preserve cell structure. In a second step, the distribution of 99Tc in the two plant species was determined. For radiological reasons, a concentration three orders of magnitude lower was used, rendering measurements with SIMS impossible. Therefore, resonant laser secondary neutral mass spectrometry (rL-SNMS) was used for the first time to image 99Tc with suppression of molecular isobaric interferences. The measurement of only about 1010 atoms of 99Tc atoms is demonstrated and the distribution of 99Tc within a single epidermal cell is imaged.

Keywords

    Carrot, Pea, Plant uptake, SIMS, SNMS, Technetium

ASJC Scopus subject areas

Cite this

Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS. / Mandel, Marcus; Holtmann, Linus; Raiwa, Manuel et al.
In: Journal of hazardous materials, Vol. 423, 127143, 05.02.2022.

Research output: Contribution to journal › Article › Research › peer review

Mandel, M, Holtmann, L, Raiwa, M, Wunnenberg-Gust, A, Riebe, B & Walther, C 2022, 'Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS', Journal of hazardous materials, vol. 423, 127143. https://doi.org/10.1016/j.jhazmat.2021.127143
Mandel, M., Holtmann, L., Raiwa, M., Wunnenberg-Gust, A., Riebe, B., & Walther, C. (2022). Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS. Journal of hazardous materials, 423, Article 127143. https://doi.org/10.1016/j.jhazmat.2021.127143
Mandel M, Holtmann L, Raiwa M, Wunnenberg-Gust A, Riebe B, Walther C. Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS. Journal of hazardous materials. 2022 Feb 5;423:127143. Epub 2021 Sept 6. doi: 10.1016/j.jhazmat.2021.127143
Mandel, Marcus ; Holtmann, Linus ; Raiwa, Manuel et al. / Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS. In: Journal of hazardous materials. 2022 ; Vol. 423.
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abstract = "In radioecological studies, there is a significant need for understanding the plant uptake of radionuclides on a cellular level. The present work applies mass spectrometry to image the radionuclide distribution within the cellular structures of plants at varying concentrations. In a first step, plants of Daucus carota and Pisum sativum labelled with iodine and rhenium were examined, at concentrations in the range of 10 mM. Cross sections of several plant parts were imaged by secondary ion mass spectrometry (SIMS) after cryogenation in order to preserve cell structure. In a second step, the distribution of 99Tc in the two plant species was determined. For radiological reasons, a concentration three orders of magnitude lower was used, rendering measurements with SIMS impossible. Therefore, resonant laser secondary neutral mass spectrometry (rL-SNMS) was used for the first time to image 99Tc with suppression of molecular isobaric interferences. The measurement of only about 1010 atoms of 99Tc atoms is demonstrated and the distribution of 99Tc within a single epidermal cell is imaged.",
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AU - Wunnenberg-Gust, Annika

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