Details
| Original language | English |
|---|---|
| Pages (from-to) | 543-551 |
| Number of pages | 9 |
| Journal | ACS Applied Bio Materials |
| Volume | 6 |
| Issue number | 2 |
| Early online date | 6 Feb 2023 |
| Publication status | Published - 20 Feb 2023 |
| Externally published | Yes |
Abstract
Mesenchymal stem cells (MSCs) are the most prominent type of adult stem cells for clinical applications. Three-dimensional (3D) cultivation of MSCs in biomimetic hydrogels provides a more physiologically relevant cultivation microenvironment for in vitro testing and modeling, thus overcoming the limitations of traditional planar cultivation methods. Cellulose nanofibers are an excellent candidate biomaterial for synthesis of hydrogels for this application, due to their biocompatibility, tunable properties, availability, and low cost. Herein, we demonstrate the capacity of hydrogels prepared from 2,2,6,6-tetramethylpiperidine-1-oxyl -oxidized and subsequently individualized cellulose-nanofibrils to support physiologically relevant 3D in vitro cultivation of human MSCs at low solid contents (0.2-0.5 wt %). Our results show that MSCs can spread, proliferate, and migrate inside the cellulose hydrogels, while the metabolic activity and proliferative capacity of the cells as well as their morphological characteristics benefit more in the lower bulk cellulose concentration hydrogels.
Keywords
- 3D cultivation, cellulose, hydrogel, in vitro culture, mesenchymal stem cells
ASJC Scopus subject areas
- Materials Science(all)
- Biomaterials
- Chemistry(all)
- Engineering(all)
- Biomedical Engineering
- Medicine(all)
- Biochemistry, medical
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In: ACS Applied Bio Materials, Vol. 6, No. 2, 20.02.2023, p. 543-551.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Hydrogels from TEMPO-Oxidized Nanofibrillated Cellulose Support In Vitro Cultivation of Encapsulated Human Mesenchymal Stem Cells
AU - Nikolits, Ilias
AU - Radwan, Sara
AU - Liebner, Falk
AU - Dietrich, Wolf
AU - Egger, Dominik
AU - Chariyev-Prinz, Farhad
AU - Kasper, Cornelia
N1 - Funding Information: This project was supported by the Doctoral School “Biomaterials and Biointerfaces (BioMatInt)” of the University of Natural Resources and Life Sciences BOKU Vienna.
PY - 2023/2/20
Y1 - 2023/2/20
N2 - Mesenchymal stem cells (MSCs) are the most prominent type of adult stem cells for clinical applications. Three-dimensional (3D) cultivation of MSCs in biomimetic hydrogels provides a more physiologically relevant cultivation microenvironment for in vitro testing and modeling, thus overcoming the limitations of traditional planar cultivation methods. Cellulose nanofibers are an excellent candidate biomaterial for synthesis of hydrogels for this application, due to their biocompatibility, tunable properties, availability, and low cost. Herein, we demonstrate the capacity of hydrogels prepared from 2,2,6,6-tetramethylpiperidine-1-oxyl -oxidized and subsequently individualized cellulose-nanofibrils to support physiologically relevant 3D in vitro cultivation of human MSCs at low solid contents (0.2-0.5 wt %). Our results show that MSCs can spread, proliferate, and migrate inside the cellulose hydrogels, while the metabolic activity and proliferative capacity of the cells as well as their morphological characteristics benefit more in the lower bulk cellulose concentration hydrogels.
AB - Mesenchymal stem cells (MSCs) are the most prominent type of adult stem cells for clinical applications. Three-dimensional (3D) cultivation of MSCs in biomimetic hydrogels provides a more physiologically relevant cultivation microenvironment for in vitro testing and modeling, thus overcoming the limitations of traditional planar cultivation methods. Cellulose nanofibers are an excellent candidate biomaterial for synthesis of hydrogels for this application, due to their biocompatibility, tunable properties, availability, and low cost. Herein, we demonstrate the capacity of hydrogels prepared from 2,2,6,6-tetramethylpiperidine-1-oxyl -oxidized and subsequently individualized cellulose-nanofibrils to support physiologically relevant 3D in vitro cultivation of human MSCs at low solid contents (0.2-0.5 wt %). Our results show that MSCs can spread, proliferate, and migrate inside the cellulose hydrogels, while the metabolic activity and proliferative capacity of the cells as well as their morphological characteristics benefit more in the lower bulk cellulose concentration hydrogels.
KW - 3D cultivation
KW - cellulose
KW - hydrogel
KW - in vitro culture
KW - mesenchymal stem cells
UR - http://www.scopus.com/inward/record.url?scp=85147823505&partnerID=8YFLogxK
U2 - 10.1021/acsabm.2c00854
DO - 10.1021/acsabm.2c00854
M3 - Article
C2 - 36745634
AN - SCOPUS:85147823505
VL - 6
SP - 543
EP - 551
JO - ACS Applied Bio Materials
JF - ACS Applied Bio Materials
IS - 2
ER -